Novel regulators of airway epithelial barrier function during inflammation: potential targets for drug repurposing

Sunil, Ahsan Anjoom; Skaria, Tom

doi:10.1080/14728222.2022.2035720

Cited by 1 publication

(1 citation statement)

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“…The structure and function of the airway epithelium as a barrier between tissues and inhaled air is controlled by the composition of the local extracellular matrix (ECM) and by epithelial cell tight junction proteins [ 4 ]. The local composition of the ECM is subject to constant degradation and de novo synthesis, which is largely regulated through various cytokines and growth factors modulated by rhinovirus (RV), such as the transforming growth factor (TGF)-β1 [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

EPs® 7630 Stimulates Tissue Repair Mechanisms and Modifies Tight Junction Protein Expression in Human Airway Epithelial Cells

Fang

Zhou

Kulić

et al. 2023

IJMS

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Airway epithelium repair after infection consists of wound repair, re-synthesis of the extracellular matrix (ECM), and tight junction proteins. In humans, EPs® 7630 obtained from Pelargonium sidoides roots reduces the severity and duration of acute respiratory tract infections. The effect of EPs® 7630 on tissue repair of rhinovirus-16 (RV-16) infected and control human airway epithelial cells was assessed for: (i) epithelial cell proliferation by manual cell counts, (ii) epithelial wound repair by “scratch assay”, (iii) ECM composition by Western-blotting and cell-based ELISA, and (iv) epithelial tight junction proteins by Western-blotting. EPs® 7630 stimulated cell proliferation through cAMP, CREB, and p38 MAPK. EPs® 7630 significantly improved wound repair. Pro-inflammatory collagen type-I expression was reduced by EPs® 7630, while fibronectin was increased. Virus-binding tight junction proteins desmoglein2, desmocollin2, ZO-1, claudin1, and claudin4 were downregulated by EPs® 7630. The RV16-induced shift of the ECM towards the pro-inflammatory type was prevented by EPs® 7630. Most of the effects of EPs® 7630 on tissue repair and regeneration were sensitive to inhibition of cAMP-induced signaling. The data suggest that EPs® 7630-dependent modification of epithelial cell metabolism and function might underlie the faster recovery time from viral infections, as reported by others in clinical studies.

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Section: Introductionmentioning

confidence: 99%