Novel Regulators in Photosynthetic Redox Control of Plant Metabolism and Gene Expression

Dietz, Karl‐Josef; Pfannschmidt, Thomas

doi:10.1104/pp.110.170043

Cited by 179 publications

(151 citation statements)

References 48 publications

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“…Our findings that increased redox poise of the ACHT1 and 2-Cys Prx pathway decreased the photosynthetic ETR with a concomitant increase in NPQ during the transition from dark to light, but maintained normal levels of chlorophylls and anthocyanins and Fv/Fm values (Figures 5 and 8), imply a redoxbased feedback regulation of the thylakoid electron transfer reactions. Numerous studies have assigned a marked role to the intersystem electron carriers, especially the plastoquinone pool and the cytochrome b 6 f, in the control of the electron flow reactions through regulation of light-harvesting complex II (LHCII) phosphorylation and NPQ (reviewed in Niyogi, 1999;Foyer and Noctor, 2009;Dietz and Pfannschmidt, 2011;Rochaix, 2011). Both changes in the redox state of the plastoquinone pool and DpH signals are used to regulate these phenomena.…”

Section: Discussionmentioning

confidence: 99%

A Chloroplast Light-Regulated Oxidative Sensor for Moderate Light Intensity in Arabidopsis

et al. 2012

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The transition from dark to light involves marked changes in the redox reactions of photosynthetic electron transport and in chloroplast stromal enzyme activity even under mild light and growth conditions. Thus, it is not surprising that redox regulation is used to dynamically adjust and coordinate the stromal and thylakoid compartments. While oxidation of regulatory proteins is necessary for the regulation, the identity and the mechanism of action of the oxidizing pathway are still unresolved. Here, we studied the oxidation of a thylakoid-associated atypical thioredoxin-type protein, ACHT1, in the Arabidopsis thaliana chloroplast. We found that after a brief period of net reduction in plants illuminated with moderate light intensity, a significant oxidation reaction of ACHT1 arises and counterbalances its reduction. Interestingly, ACHT1 oxidation is driven by 2-Cys peroxiredoxin (Prx), which in turn eliminates peroxides. The ACHT1 and 2-Cys Prx reaction characteristics in plants further indicated that ACHT1 oxidation is linked with changes in the photosynthetic production of peroxides. Our findings that plants with altered redox poise of the ACHT1 and 2-Cys Prx pathway show higher nonphotochemical quenching and lower photosynthetic electron transport infer a feedback regulatory role for this pathway.

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Section: Discussionmentioning

confidence: 99%

A Chloroplast Light-Regulated Oxidative Sensor for Moderate Light Intensity in Arabidopsis

et al. 2012

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“…2001; Wulff‐Zottele et al . 2010; Dietz & Pfannschmidt 2011). In the longer term, changes in weather patterns can give rise to sustained changes in ambient light (e.g.…”

Section: Introductionmentioning

confidence: 99%

Acclimation of metabolism to light in Arabidopsis thaliana: the glucose 6‐phosphate/phosphate translocator GPT2 directs metabolic acclimation

Dyson

Allwood

Feil

et al. 2015

Plant Cell & Environment

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Mature leaves of plants transferred from low to high light typically increase their photosynthetic capacity. In A rabidopsis thaliana, this dynamic acclimation requires expression of GPT2, a glucose 6‐phosphate/phosphate translocator. Here, we examine the impact of GPT2 on leaf metabolism and photosynthesis. Plants of wild type and of a GPT2 knockout (gpt2.2) grown under low light achieved the same photosynthetic rate despite having different metabolic and transcriptomic strategies. Immediately upon transfer to high light, gpt2.2 plants showed a higher rate of photosynthesis than wild‐type plants (35%); however, over subsequent days, wild‐type plants acclimated photosynthetic capacity, increasing the photosynthesis rate by 100% after 7 d. Wild‐type plants accumulated more starch than gpt2.2 plants throughout acclimation. We suggest that GPT2 activity results in the net import of glucose 6‐phosphate from cytosol to chloroplast, increasing starch synthesis. There was clear acclimation of metabolism, with short‐term changes typically being reversed as plants acclimated. Distinct responses to light were observed in wild‐type and gpt2.2 leaves. Significantly higher levels of sugar phosphates were observed in gpt2.2. We suggest that GPT2 alters the distribution of metabolites between compartments and that this plays an essential role in allowing the cell to interpret environmental signals.

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“…It is well established that reversible phosphorylation and reduction/oxidation (e.g. through the action of different types of plastid thioredoxins) are key regulators of plastid metabolism as well as plastid gene expression (Dietz and Pfannschmidt, 2010). Several proteomics studies identified thioredoxin targets by affinity chromatography, whereas other redox proteomics approaches used diagonal electrophoresis under reducing and oxidizing conditions to identify proteins under redox control in vivo (Dietz and Pfannschmidt, 2010).…”

Section: Reversible and Irreversible Ptmsmentioning

confidence: 99%

“…Redox targets in the thylakoid lumen were identified, and inhibition of the activity of the xanthophyll cycle enzyme violaxanthin deepoxidase by reduction (i.e. dithiol generation) was established (Dietz and Pfannschmidt, 2010).…”

Section: Reversible and Irreversible Ptmsmentioning

confidence: 99%

Plastid Proteomics in Higher Plants: Current State and Future Goals

Wijk

Baginsky

2011

Plant Physiology

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Novel Regulators in Photosynthetic Redox Control of Plant Metabolism and Gene Expression

Cited by 179 publications

References 48 publications

A Chloroplast Light-Regulated Oxidative Sensor for Moderate Light Intensity in Arabidopsis

A Chloroplast Light-Regulated Oxidative Sensor for Moderate Light Intensity in Arabidopsis

Acclimation of metabolism to light in Arabidopsis thaliana: the glucose 6‐phosphate/phosphate translocator GPT2 directs metabolic acclimation

Plastid Proteomics in Higher Plants: Current State and Future Goals

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