Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strain

Brenelli, Lívia Beatriz; Persinoti, Gabriela Félix; Cairo, João Paulo L. Franco; Liberato, M.V.; Gonçalves, Thiago Augusto; Otero, Igor Vinicius Ramos; Mainardi, Pedro Henrique; Felby, Claus; Sette, Lara Durães; Squina, Fábio M.

doi:10.1038/s41598-019-53608-1

Cited by 27 publications

(16 citation statements)

References 61 publications

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“…Peniophora sp. was recovered from rhizosphere soil, Brazilian sponge A.viridis and the mangrove Bruguiera gymnorrhiza in South China (Gonçalves et al 2015 ; Brenelli et al 2019 ; Li et al 2020 ). This species has not been reported from macroalgae in Mauritius.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial properties of marine fungi from sponges and brown algae of Mauritius

et al. 2021

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Purpose of the study: Marine fungi of Mauritius have been poorly studied. There are numerous reports on the bioactive secondary metabolites that are produced by fungi around the world. Yet, research on the molecular characterisation and the pharmaceutical potential of marine fungi in Mauritius is rather scanty. Method: The samples, which consisted of three sponges Haliclona sp., Iotrochota sp. and Biemna sp. and two brown algae Turbinaria conoides and Sargassum portierianum, were collected in the North of Mauritius during winter. No sporulating structures were observed from the fungal cultures making morphological analysis impossible. The molecular characterisation of the selected isolates was carried out by the amplification of the ITS regions and phylogenetic analysis. The antimicrobial properties were then determined using the disc diffusion and the minimum inhibitory concentration (MIC) assay. Results: Genus level identification was made from molecular data and for some isolates, specieslevel identification was even possible. Twelve fungi that showed the best antimicrobial properties were identified as Peniophora sp., Aspergillus cristatus, Acremonium sp., Cordyceps memorabilis, Aspergillus ochraceus, Biscogniauxia sp., Aspergillus keratitidis, Exserohilum rostratum, Chromocleista sp., Nigrospora oryzae, Aspergillus flavipes and Mycosphaerella. The lowest MIC result of 0.0098 mg/ mL was obtained with Chromocleista sp. mycelium extract against Staphylococcus aureus. The MIC of the mycelium extracts was lower than the broth extracts for most isolates indicating that the antimicrobial compounds are not secreted. Conclusion:Marine fungi from the Mauritian waters have immense potential in the search for natural products against antibiotic-resistant bacteria.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial properties of marine fungi from sponges and brown algae of Mauritius

et al. 2021

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“…AA1 is a multicopper oxidase with activities including laccase, ferroxidase, and laccase-like multicopper oxidase while AA5 is a copper radical oxidase [68]. Both these subfamilies are known to have the ability to oxidize a wide range of compounds including lignin [69]. AA9 (formerly GH61), a lytic polysaccharide monooxygenases (LPMOs), acts directly on cellulose and works synergistically with other cellulose enzymes to enhance the hydrolysis of cellulose producing substrates that are accessible to other CAZy [70] (Table 4).…”

Section: Cazy Distribution Analysismentioning

confidence: 99%

Comparative Genomics: Insights on the Pathogenicity and Lifestyle of Rhizoctonia solani

Razali

Hisham

Kumar

et al. 2021

IJMS

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Proper management of agricultural disease is important to ensure sustainable food security. Staple food crops like rice, wheat, cereals, and other cash crops hold great export value for countries. Ensuring proper supply is critical; hence any biotic or abiotic factors contributing to the shortfall in yield of these crops should be alleviated. Rhizoctonia solani is a major biotic factor that results in yield losses in many agriculturally important crops. This paper focuses on genome informatics of our Malaysian Draft R. solani AG1-IA, and the comparative genomics (inter- and intra- AG) with four AGs including China AG1-IA (AG1-IA_KB317705.1), AG1-IB, AG3, and AG8. The genomic content of repeat elements, transposable elements (TEs), syntenic genomic blocks, functions of protein-coding genes as well as core orthologous genic information that underlies R. solani’s pathogenicity strategy were investigated. Our analyses show that all studied AGs have low content and varying profiles of TEs. All AGs were dominant for Class I TE, much like other basidiomycete pathogens. All AGs demonstrate dominance in Glycoside Hydrolase protein-coding gene assignments suggesting its importance in infiltration and infection of host. Our profiling also provides a basis for further investigation on lack of correlation observed between number of pathogenicity and enzyme-related genes with host range. Despite being grouped within the same AG with China AG1-IA, our Draft AG1-IA exhibits differences in terms of protein-coding gene proportions and classifications. This implies that strains from similar AG do not necessarily have to retain similar proportions and classification of TE but must have the necessary arsenal to enable successful infiltration and colonization of host. In a larger perspective, all the studied AGs essentially share core genes that are generally involved in adhesion, penetration, and host colonization. However, the different infiltration strategies will depend on the level of host resilience where this is clearly exhibited by the gene sets encoded for the process of infiltration, infection, and protection from host.

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“…Currently, only for Peniophora sp. CBMAI data about the genome, transcriptome, secretome, and the main secreted laccase are available [19,22,29,58]. Based on the transcriptomic data, it was previously shown that Peniophora sp.…”

Section: Orthology Groups Of Laccases From Fungi Of Peniophora Genusmentioning

confidence: 99%

“…Currently, only 11 published articles can be found about laccases from fungi of the Peniophora genus [18][19][20][21][22][23][24][25][26][27][28]. Worse than that, almost all these works are strictly technically oriented and use only crude laccase preparations: only in three works [20,22,28] laccases were purified; only in four works [20,21,25,26] used fungi were identified to the species level; only in two works [21,29] fungi were properly genetically barcoded; and only in three works [25,28,29] either full or partial sequences of used laccases were obtained. This situation enormously complicates any comparative studies and raises serious reproducibility issues.…”

Section: Introductionmentioning

confidence: 99%

Purification and Characterization of Two Novel Laccases from Peniophora lycii

Glazunova

Moiseenko

Savinova

et al. 2020

JoF

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Although, currently, more than 100 laccases have been purified from basidiomycete fungi, the majority of these laccases were obtained from fungi of the Polyporales order, and only scarce data are available about the laccases from other fungi. In this article, laccase production by the white-rot basidiomycete fungus Peniophora lycii, belonging to the Russulales order, was investigated. It was shown that, under copper induction, this fungus secreted three different laccase isozymes. Two laccase isozymes—Lac5 and LacA—were purified and their corresponding nucleotide sequences were determined. Both purified laccases were relatively thermostable with periods of half-life at 70 °C of 10 and 8 min for Lac5 and LacA, respectively. The laccases demonstrated the highest activity toward ABTS (97 U·mg−1 for Lac5 and 121 U·mg−1 for LacA at pH 4.5); Lac5 demonstrated the lowest activity toward 2,6-DMP (2.5 U·mg−1 at pH 4.5), while LacA demonstrated this towards gallic acid (1.4 U·mg−1 at pH 4.5). Both Lac5 and LacA were able to efficiently decolorize such dyes as RBBR and Bromcresol Green. Additionally, phylogenetic relationships among laccases of Peniophora spp. were reconstructed, and groups of orthologous genes were determined. Based on these groups, all currently available data about laccases of Peniophora spp. were systematized.

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Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strain

Cited by 27 publications

References 61 publications

Antimicrobial properties of marine fungi from sponges and brown algae of Mauritius

Antimicrobial properties of marine fungi from sponges and brown algae of Mauritius

Comparative Genomics: Insights on the Pathogenicity and Lifestyle of Rhizoctonia solani

Purification and Characterization of Two Novel Laccases from Peniophora lycii

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