Novel recombinant adenovirus type 41 vector and its biological properties

Zz, Lu; Zou, Xiaohui; Dong, Liuxin; Qu, Junle; Song, Jingdong; Wang, Min; Guo, Li; Hung, Tao

doi:10.1002/jgm.1284

Cited by 23 publications

(23 citation statements)

References 39 publications

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“…In contrast to the aggregation between pH 4.0 and 4.7, at pH 2.8, the virion hydrodynamic diameter was only 112 Ϯ 20 nm, suggesting minimal aggregation. This is consistent with studies showing that the enteric adenoviruses remain infective under the acidic conditions in the gut (49,50). A high positive -potential at low pH (Fig.…”

Section: Resultssupporting

confidence: 91%

Elution Is a Critical Step for Recovering Human Adenovirus 40 from Tap Water and Surface Water by Cross-Flow Ultrafiltration

Shi

Xagoraraki

Parent

et al. 2016

Appl Environ Microbiol

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This paper examines the recovery of the enteric adenovirus human adenovirus 40 (HAdV 40) by cross-flow ultrafiltration and interprets recovery values in terms of physicochemical interactions of virions during sample concentration. Prior to ultrafiltration, membranes were either blocked by exposure to calf serum (CS) or coated with a polyelectrolyte multilayer (PEM). HAdV 40 is a hydrophobic virus with a point of zero charge between pH 4.0 and pH 4.3. In accordance with predictions from the extended Derjaguin-Landau-Verwey-Overbeek theory, the preelution recovery of HAdV (r pre ) from deionized water was higher with PEMcoated membranes (r pre PEM ‫؍‬ 74.8% ؎ 9.7%) than with CS-blocked membranes (r pre CS ‫؍‬ 54.1% ؎ 6.2%). With either membrane type, the total virion recovery after elution (r post ) was high for both deionized water (r post PEM ‫؍‬ 99.5% ؎ 6.6% and r post CS ‫؍‬ 98.8% ؎ 7.7%) and tap water (r post PEM ‫؍‬ 89% ؎ 15% and r post CS ‫؍‬ 93.7% ؎ 6.9%). The nearly 100% recoveries suggest that the polyanion (sodium polyphosphate) and surfactant (Tween 80) in the eluent disrupt electrostatic and hydrophobic interactions between the virion and the membrane. Addition of EDTA to the eluent greatly improved the elution efficacy (r post CS ‫؍‬ 88.6% ؎ 4.3% and r post PEM ‫؍‬ 87.0% ؎ 6.9%) with surface water, even when the organic carbon concentration in the water was high (9.4 ؎ 0.1 mg/liter). EDTA likely disrupts cation bridging between virions and particles in the feed water matrix or the fouling layer on the membrane surface. For complex water matrices, the eluent composition is the most important factor for achieving high virion recovery. IMPORTANCEHerein we present the results of a comprehensive physicochemical characterization of HAdV 40, an important human pathogen. The data on HAdV 40 surface properties enabled rigorous modeling to gain an understanding of the energetics of virion-virion and virion-filter interactions. Cross-flow filtration for concentration and recovery of HAdV 40 was evaluated, with postelution recoveries from ultrapure water (99%), tap water (ϳ91%), and high-carbon-content surface water (ϳ84%) being demonstrated. These results are significant because of the very low adenovirus recoveries that have been reported, to date, for other methods. The recovery data were interpreted in terms of specific interactions, and the eluent composition was designed accordingly to maximize HAdV 40 recovery. P athogenic microorganisms cause many waterborne diseases, and the World Health Organization reports that water supply contamination leads to 842,000 deaths annually (1). Along with caliciviruses (e.g., norovirus), enteroviruses (e.g., poliovirus, coxsackievirus, and echovirus), and hepatitis A viruses, human adenovirus (HAdV) is one of the viral pathogens in EPA contaminant candidate lists 3 and 4 and is the second-leading cause of childhood gastroenteritis worldwide (2-4). Most cases of adenovirusassociated gastroenteritis stem from HAdV serotypes 40 and 41 (5), which are relatively resis...

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Section: Resultssupporting

confidence: 91%

Elution Is a Critical Step for Recovering Human Adenovirus 40 from Tap Water and Surface Water by Cross-Flow Ultrafiltration

Shi

Xagoraraki

Parent

et al. 2016

Appl Environ Microbiol

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“…Wild-type HAdV-41, which originated from a fecal sample (NIVD103), has been described elsewhere [18]. pAd41-GFP, a plasmid that was constructed previously [18], contains the whole genome of HAdV-41 except the E1 region, which was replaced by a GFP gene.…”

Section: Methodsmentioning

confidence: 99%

“…pKAd41 was partially sequenced. The sequence information for the left end of the genome was combined with that of pAd41-GFP, which was sequenced previously [18], to generate the complete genome sequence (GenBank accession no. HM565136.3).…”

Section: Cloning Of the Hadv-41 Genome And Rescue Of The Virusmentioning

confidence: 99%

“…The relationship of the phenotype of HAdV-F to gut tropism has been studied in vitro. For example, HAdV-F was found to be resistant to acid and digestive enzymes, and it tends to interact with lipids on the surface of gastric and intestinal mucosa [3,6,18]. However, the molecular mechanism behind these phenomena remains largely unknown, partly due to the fastidiousness of this virus.…”

Section: Introductionmentioning

confidence: 96%

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Construction of an infectious clone of human adenovirus type 41

Chen

Dong

et al. 2012

Arch Virol

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Human adenovirus type 41 (HAdV-41) is well known for its fastidiousness in cell culture. To construct an infectious clone of HAdV-41, a DNA fragment containing the left and right ends of HAdV-41 as well as a kanamycin resistance gene and a pBR322 replication origin was excised from the previously constructed plasmid pAd41-GFP. Using homologous recombination, the plasmid pKAd41 was generated by co-transformation of the E. coli BJ5183 strain with this fragment and HAdV-41 genomic DNA. Virus was rescued from pKAd41-transfected 293TE7 cells, a HAdV-41 E1B55K-expressing cell line. The genomic integrity of the rescued virus was verified by restriction analysis and sequencing. Two fibers on the virion were confirmed by western blot. Immunofluorescence showed that more expression of the hexon protein could be found in 293TE7 cells than in 293 cells after HAdV-41 infection. The feature of non-lytic replication was preserved in 293TE7 cells, since very few progeny HAdV-41 viruses were released to the culture medium. These results show that pKAd41 is an effective infectious clone and suggest that the combination of pKAd41 and 293TE7 cells is an ideal system for virological study of HAdV-41.

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“…Adenovirus is an ideal carrier [2]. This recombinant replication-defective adenovirus vector ( pAd/CMV/V5-DEST) could be used for gene therapy in tumors and has vast improvements compared with the previous generation of adenovirus vector [3]. There are lots of advantages to transfect with this vector both in vitro and in vivo, such as high transfection efficiency, low toxicity, weak immunogenicity, and long periods of gene product expression.…”

mentioning

confidence: 99%

Construction of recombinant adenovirus vector with connexin 43 gene

Wu¹,

Liu²,

Li³

et al. 2012

ABBS

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Novel recombinant adenovirus type 41 vector and its biological properties

Abstract: An Ad41 vector system was successfully constructed, which consisted of the backbone plasmid, shuttle plasmid and packaging cell line 293E12. This system can be utilized to generate genetically stable and acid-resistant recombinant Ad41 carrying any gene of interest.

Cited by 23 publications

References 39 publications

Elution Is a Critical Step for Recovering Human Adenovirus 40 from Tap Water and Surface Water by Cross-Flow Ultrafiltration

Elution Is a Critical Step for Recovering Human Adenovirus 40 from Tap Water and Surface Water by Cross-Flow Ultrafiltration

Construction of an infectious clone of human adenovirus type 41

Construction of recombinant adenovirus vector with connexin 43 gene

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