Novel Reassortant Influenza A(H5N8) Viruses, South Korea, 2014

Lee, Youn‐Jeong; Kang, Hyun-Mi; Lee, Eun‐Kyoung; Song, Bingbing; Jeong, Junhui; Kwon, Yong-Kuk; Kim, Hye-Ryoung; Lee, Kyu-Jun; Hong, Mi-Seon; Jang, Il; Choi, Kang-Seuk; Kim, Ji-Ye; Lee, Hyun-Jeong; Kang, Min-Su; Jeong, Ok-Mi; Baek, Jong-Ho; Joo, Yi-Seok; Park, Taesung; Lee, Heesoo

doi:10.3201/eid2006.140233

Cited by 262 publications

(247 citation statements)

References 8 publications

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“…The anti-N8 antibodies generated by our fusion also showed a very broad binding profile; over half of the isolated MAbs exhibited binding to both the North American and the Eurasian N8 lineages. Importantly, we found strong reactivity to potential pandemic viruses like H3N8, which caused a severe outbreak in harbor seals in New England in 2012, as well as against the N8 NA of the newly emerging, highly pathogenic H5N8 viruses (26,30,31,36,37). The H3N8 subtype is also important, since it was speculated to be the cause of a human pandemic in 1889 (38).…”

Section: Discussionmentioning

confidence: 95%

Hemagglutinin Stalk- and Neuraminidase-Specific Monoclonal Antibodies Protect against Lethal H10N8 Influenza Virus Infection in Mice

Wohlbold

Chromikova

Tan

et al. 2016

J Virol

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Between November 2013 and February 2014, China reported three human cases of H10N8 influenza virus infection in the Jiangxi province, two of which were fatal. Using hybridoma technology, we isolated a panel of H10-and N8-directed monoclonal antibodies (MAbs) and further characterized the binding reactivity of these antibodies (via enzyme-linked immunosorbent assay) to a range of purified virus and recombinant protein substrates. The H10-directed MAbs displayed functional hemagglutination inhibition (HI) and neutralization activity, and the N8-directed antibodies displayed functional neuraminidase inhibition (NI) activity against H10N8. Surprisingly, the HI-reactive H10 antibodies, as well as a previously generated, group 2 hemagglutinin (HA) stalk-reactive antibody, demonstrated NI activity against H10N8 and an H10N7 strain; this phenomenon was absent when virus was treated with detergent, suggesting the anti-HA antibodies inhibited neuraminidase enzymatic activity through steric hindrance. We tested the prophylactic efficacy of one representative H10-reactive, N8-reactive, and group 2 HA stalk-reactive antibody in vivo using a BALB/c challenge model. All three antibodies were protective at a high dose (5 mg/kg). At a low dose (0.5 mg/kg), only the anti-N8 antibody prevented weight loss. Together, these data suggest that antibody targets other than the globular head domain of the HA may be efficacious in preventing influenza virus-induced morbidity and mortality. IMPORTANCEAvian H10N8 and H10N7 viruses have recently crossed the species barrier, causing morbidity and mortality in humans and other mammals. Although these reports are likely isolated incidents, it is possible that more cases may emerge in future winter seasons, similar to H7N9. Furthermore, regular transmission of avian influenza viruses to humans increases the risk of adaptive mutations and reassortment events, which may result in a novel virus with pandemic potential. Currently, no specific therapeutics or vaccines are available against the H10N8 influenza virus subtype. We generated a panel of H10-and N8-reactive MAbs. Although these antibodies may practically be developed into therapeutic agents, characterizing the protective potential of MAbs that have targets other than the HA globular head domain will provide insight into novel antibody-mediated mechanisms of protection and help to better understand correlates of protection for influenza A virus infection. R ecently, avian influenza A viruses of the H10 subtype have been reported to infect seals and humans and have generated concern over their pandemic potential. Three human cases of H10N8 virus have been reported in China so far, two of which were fatal (1-3). Furthermore, an avian H10N7 strain was found to be the etiological agent responsible for the massive die-off harbor seals in the Baltic Sea, an epidemic that killed more than 10% of the local seal population (4-6). The receptor binding profile of H10 viruses is currently debated (7-12), but the subtype has been proven to cause ...

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Section: Discussionmentioning

confidence: 95%

Hemagglutinin Stalk- and Neuraminidase-Specific Monoclonal Antibodies Protect against Lethal H10N8 Influenza Virus Infection in Mice

Wohlbold

Chromikova

Tan

et al. 2016

J Virol

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“…99 to 100% identical). Two genetic lineages (A and B) of H5N8 virus were identified in both domestic and wild birds from South Korea in January 2014, of which lineage A was more frequently detected in both domestic Table 2B Information on influenza A virus sequences obtained from the Global Initiative on Sharing Avian Influenza Data used for the study and wild birds [7,8,16]. H5N8 viruses detected in Europe (Germany, Italy, the Netherlands, and the UK), Russia and in North America belonged to lineage A based on analyses of the HA gene [8].…”

Section: Genetic Analyses Of H5n8 Virusesmentioning

confidence: 99%

Wild bird surveillance around outbreaks of highly pathogenic avian influenza A(H5N8) virus in the Netherlands, 2014, within the context of global flyways

et al. 2015

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Highly pathogenic avian influenza (HPAI) A(H5N8) viruses that emerged in poultry in east Asia since 2010 spread to Europe and North America by late 2014. Despite detections in migrating birds, the role of free-living wild birds in the global dispersal of H5N8 virus is unclear. Here, wild bird sampling activities in response to the H5N8 virus outbreaks in poultry in the Netherlands are summarised along with a review on ring recoveries. HPAI H5N8 virus was detected exclusively in two samples from ducks of the Eurasian wigeon species, among 4,018 birds sampled within a three months period from mid-November 2014. The H5N8 viruses isolated from wild birds in the Netherlands were genetically closely related to and had the same gene constellation as H5N8 viruses detected elsewhere in Europe, in Asia and in North America, suggesting a common origin. Ring recoveries of migratory duck species from which H5N8 viruses have been isolated overall provide evidence for indirect migratory connections between East Asia and Western Europe and between East Asia and North America. This study is useful for better understanding the role of wild birds in the global epidemiology of H5N8 viruses. The need for sampling large numbers of wild birds for the detection of H5N8 virus and H5N8-virus-specific antibodies in a variety of species globally is highlighted, with specific emphasis in north-eastern Europe, Russia and northern China.

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“…In January 2014, newly discovered highly pathogenic H5N8 avian influenza viruses (H5N8 HPAIVs) caused outbreaks in poultry and wild birds in South Korea [1], although their ancestor had been isolated in China in 2013 [2]. Thereafter, these viruses have been circulating in both avian populations in South Korea [3,4] and sporadically in neighbouring countries, including China and Japan.…”

mentioning

confidence: 99%

Genetic diversity of highly pathogenic H5N8 avian influenza viruses at a single overwintering site of migratory birds in Japan, 2014/15

et al. 2015

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We isolated eight highly pathogenic H5N8 avian influenza viruses (H5N8 HPAIVs) in the 2014/15 winter season at an overwintering site of migratory birds in Japan. Genetic analyses revealed that these isolates were divided into three groups, indicating the co-circulation of three genetic groups of H5N8 HPAIV among these migratory birds. These results also imply the possibility of global redistribution of the H5N8 HPAIVs via the migration of these birds next winter.

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Novel Reassortant Influenza A(H5N8) Viruses, South Korea, 2014

Cited by 262 publications

References 8 publications

Hemagglutinin Stalk- and Neuraminidase-Specific Monoclonal Antibodies Protect against Lethal H10N8 Influenza Virus Infection in Mice

Hemagglutinin Stalk- and Neuraminidase-Specific Monoclonal Antibodies Protect against Lethal H10N8 Influenza Virus Infection in Mice

Wild bird surveillance around outbreaks of highly pathogenic avian influenza A(H5N8) virus in the Netherlands, 2014, within the context of global flyways

Genetic diversity of highly pathogenic H5N8 avian influenza viruses at a single overwintering site of migratory birds in Japan, 2014/15

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