Novel reagent for the fluorometric assay of primary amines

Weigele, Manfred; DeBernardo, Silvano; Tengi, J. P.; Leimgruber, W.

doi:10.1021/ja00771a084

Cited by 478 publications

(122 citation statements)

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“…We further tried to estimate the number of DOTA residues in G3-DL-DOTA by evaluating the number of the residual primary amine groups. T he fluorescamine assay [40] revealed that the number of primary amines remaining in G3-DL-DOTA molecule was estimated to be 0.12 using a calibration curve made with G3-DL as the standard. This result suggests that essentially every chain terminal of the dendron moiety was combined with DOTA residue in G3-DL-DOTA.…”

Section: Preparation Of Dendron-based Lipid Having Gd Chelatesmentioning

confidence: 99%

Multi-functional liposomes having temperature-triggered release and magnetic resonance imaging for tumor-specific chemotherapy

Kono

Nakashima

Kokuryo³

et al. 2011

Biomaterials

111

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Section: Preparation Of Dendron-based Lipid Having Gd Chelatesmentioning

confidence: 99%

Multi-functional liposomes having temperature-triggered release and magnetic resonance imaging for tumor-specific chemotherapy

Kono

Nakashima

Kokuryo³

et al. 2011

Biomaterials

111

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“…Protein concentrations were determined by the Lowry method [ 191 but where protein concentrations were very low the fluorescarnine (fluram) method was used [20].…”

Section: Protein Determinationsmentioning

confidence: 99%

Metalloproteinase inhibitors from bovine cartilage and body fluids

Bunning

Murphy

Kumar

et al. 1984

European Journal of Biochemistry

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Inhibitors of the mammalian metalloproteinases, collagenase, proteoglycanase and gelatinase were isolated from bovine cartilage (extracts and culture medium) and bovine amniotic fluid and serum. These inhibitors either bind or do not bind to concanavalin‐A – Sepharose, with Mr (gel filtration) of about 30000 and 20000, respectively. Cartilage and chondrocyte culture media contained only concanavalin‐A‐binding inhibitors whereas cartilage extracts contained only a non‐binding inhibitor: serum and amniotic fluid contained both forms of inhibitory activities. In most biochemical respects, particularly in their abilities to inhibit metalloproteinases, all of the inhibitors were found to be similar. It is concluded that the forms of the inhibitors that differ in Mr may be closely related to the tissue inhibitor of metalloproteinases (TIMP) previously purified from rabbit and human sources. These findings help to clarify other studies on collagenase inhibitors and support the concept that TIMP‐like inhibitors may be important in the control of connective tissue degradation.

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“…followed by measuring amino group with fluorescamine, 4-phenylspiro-(furan-2(3H)-l'-phthalan)-3.3'-dione [5]. At several time intervals, 0.1 ml reaction mixture was taken from the supernatant, and its pH was adjusted to 2.0 by addition of 1 N HC1.…”

Section: Introductionmentioning

confidence: 99%