Novel reactions of nitric oxide in biological systems

Gabr, I.M.; Patel, Rakesh P.; Symons, M. C. R.; Wilson, Michael T.

doi:10.1039/c39950000915

Cited by 21 publications

(16 citation statements)

References 4 publications

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“…One interesting idea was the formation of a nitroxide from the reaction between NO and a diene – so called cheletropic trapping [42]. In fact, it has been shown that NO can react with naturally occurring dienes, for example β-carotene, to generate nitroxides [43]. Cheletropic tapping has not been developed for EPR but the idea has been utilized for the fluorescent detection of NO [44].…”

Section: Other Methodsmentioning

confidence: 99%

Detection of nitric oxide by electron paramagnetic resonance spectroscopy

Hogg

2010

Free Radical Biology and Medicine

105

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Electron paramagnetic resonance (EPR) spectroscopy has been used in a number of ways to study nitric oxide chemistry and biology. As an intrinsically stable and relatively unreactive diatomic free radical, the challenges for detecting this species by EPR are somewhat different than those for transient radical species. This review gives a basic introduction to EPR spectroscopy and discusses its uses to assess and quantify nitric oxide formation in biological systems.

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Section: Other Methodsmentioning

confidence: 99%

Detection of nitric oxide by electron paramagnetic resonance spectroscopy

Hogg

2010

Free Radical Biology and Medicine

105

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“…Those Spectra shown splits in some solvents. Some authors had interpreted them as the spectrum of NO [11]. However, it was confirmed that those UV-vis spectra were obtained in polar solvents and could be attributed to the formation of HNO 2 [1,12].…”

Section: β-Carotene and Free Radical Reactions With Nitrogen Oxidesmentioning

confidence: 92%

β‐Carotene and Free Radical Reactions with Nitrogen Oxides

Mendiara¹,

Perissinotti²

2017

Carotenoids

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The following presentation is based on experimental work we have already developed and published. We investigated the nitrogen oxides in different solvents and analyzed their reaction with β-carotene. The electron paramagnetic resonance spectroscopy (EPR) and ultraviolet and visible (UV-vis) spectroscopy were applied to investigate the reaction of β-carotene with nitrogen dioxide and nitric oxide in both pure dioxane and dioxane/ water solvent. Free radicals were detected and evaluated with the EPR technique, which is highly selective and sensitive. A reaction mechanism was proposed on the basis of the experimental kinetic and EPR results. The validity of the mechanism was checked by applying simulation set up conditions that reproduced the results achieved. The radical intermediates proposed in the reaction: the β-carotene neutral radicals and the cyclic nitroxide neutral radicals were theoretically studied. For that purpose, the density functional theory (DFT) level was applied, selecting the most suitable method, the unrestricted Becke-style 3-parameter with the Lee-Yang-Parr correlation functional (UB3LYP) and the 6-31G(d) basis sets (d orbital functions). We developed an appropriate discussion on the importance of carotenoids compounds and their reactions in biological media. Also, we evaluated the role and the possible reactions of nitroxide intermediates.

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“…Even though precautions were employed to exclude oxygen from the system, the fingerprint UV spectrum was assigned to the presence of NO 2 plus N2O4 plus HNO2 as impurity was sometimes observed. Knowledge about the NO 2 UV spectrum is very important because absorption in the region of 300-400 nm has been misinterpreted as the spectrum of NO by some authors [30] no reaction took place when pure NO was present, but a trace amount of NO 2 initiated the reaction [11,12]. Some authors had described that nitric oxide reacted readily with amines [31] and thiols [32] in the presence of even traces of oxygen.…”

Section: Reaction In the Presence Of No And No Plus No 2 Mixturesmentioning

confidence: 99%

An electron paramagnetic resonance study of nitrogen dioxide dissolved in water, carbon tetrachloride and some organic compounds

2001

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Electron paramagnetic resonance spectrometry was used to characterize and measure the concentration of nitrogen dioxide dissolved in water, carbon tetrachloride, n-hexane, acetone and benzene at 290 K. Solutions were monitored by ultraviolet spectrometry. We measured the equilibrium constant for N 2 04 plus NO2 dissolved in n-hexane and carbon tetrachloride. In order to investigate the formation of organic nitroxide type intermediates, some experiments were designed to examine the role of NO and NO plus NO 2 mixtures.

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Novel reactions of nitric oxide in biological systems

Abstract: The reaction between nitric oxide and the naturally occurring carotenoid (3-carotene is shown by EPR and optical spectroscopy to produce a series of nitroxides with concomitant loss in conjugation of the (3-carotene molecule, with a rate constant of ca. lo3 dm3 mol-1.

Cited by 21 publications

References 4 publications

Detection of nitric oxide by electron paramagnetic resonance spectroscopy

Detection of nitric oxide by electron paramagnetic resonance spectroscopy

β‐Carotene and Free Radical Reactions with Nitrogen Oxides

An electron paramagnetic resonance study of nitrogen dioxide dissolved in water, carbon tetrachloride and some organic compounds

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