Novel (R)-oxynitrilase sources for the synthesis of (R)-cyanohydrins in diisopropyl ether

Preparation and reactions of optically active cyanohydrins using the (R)-hydroxynitrile lyase from Prunus amygdalus

“…The (R)-hydroxynitrilase (HNL) was extracted [45] and assayed [46] according to established references. Naproxen is commercially available and naproxen chloride is prepared according to a procedure developed by Solis et al [37].…”

Biosynthesis of chiral methyl ketone cyanohydrins catalyzed by oxynitrilase in Chaenomeles speciosa seed meal

“…As to the preparation of Chaenomeles Speciosa seed meal, we can refer to the method about apple seed meal described in reference [10]. A typical procedure for the enzyme-mediated hydrocyanation of methyl ketones is as follows: To a mixture of citrate buffer (1.5 mL, 0.1 M, pH 4.0) and the powered, defatted Chaenomeles Speciosa seed meal (250 mg) methyl tert-butyl ether (6.8 mL), freshly distilled methyl ketone (2 mmol) and acetone cyanohydrin (0.46 mL, 5 mmol) are subsequently added.…”

Preparation and Reactions of Certain Racemic and Optically Active Cyanohydrins Derived from 2-Chlorobenzaldehyde, 4-Fluorobenzaldehyde, Benzo[d][1,3]-dioxole-5-carbaldehyde and 2,3-Dihydrobenzo[b][1,4]dioxine-6-carbaldehyde. Antimicrobial and in vitro Antitumor Evaluation of the Products

“…The almond meal and the isolated cyanohydrins were stored at -15 °C. (R)-Oxynitrilase enzy me (PaHNL) [EC4.1.2.10] was ext racted [39], assayed and its activity was measured [40] according to the given procedures. pH-Values were determined by Precisa Dig ital pH-Meter pH 900 with Ag/AgCl electrode.…”

“…Step 2: Addition of Hydrogen Cyanide Solution to the aldehydes 1a -c: General Procedure [46] The ethereal solution of hydrogen cyanide was added dropwise at 0 °C under stirring by a mechanical stirrer to a mixture of the crude en zy me extract [39,40] and the appropriate aldehyde 1a-c in d iisopropyl ether (30 ml). After complet ion of the addition (30 min.…”