Novel quasi-subgenotype D2 of hepatitis B virus identified in Taiwanese aborigines

Tran, Huy; Yu, Ming‐Lung; Dai, Chia‐Yen; Lin, I‐Ling; Yeh, Ming‐Lun; Chuang, Wan‐Long; Abe, Kenji

doi:10.1007/s11262-014-1072-x

Cited by 7 publications

(8 citation statements)

References 33 publications

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“…In the present work, eight isolates belonging to HBV/D (S18014, S140014, S20014, S140914, S02E12, S2312E, S07E, and S10612E) segregated with sub-genotype D1 reference isolates, and the inter distances equaling 0.006 verified this effect. This result is in agreement with recent studies that reported the prevalence of HBV/D1 in regions of the Middle East through North Africa (EL Hadad et al, 2013, Tran et al, 2014). Additionally, a possible new HBV sub-genotype (DA) was suggested by 3 isolates, S028, S318S, and S118S, which showed identity similarities of 94−96%.…”

Section: Discussionsupporting

confidence: 94%

“…Recently, ten sub-genotypes of HBV/D (D1 to D10) have been reported, each showing distinct geographical clustering (Tran et al, 2014). In the present work, eight isolates belonging to HBV/D (S18014, S140014, S20014, S140914, S02E12, S2312E, S07E, and S10612E) segregated with sub-genotype D1 reference isolates, and the inter distances equaling 0.006 verified this effect.…”

Section: Discussionmentioning

confidence: 99%

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Sequence analysis of sub-genotype D hepatitis B surface antigens isolated from Jeddah, Saudi Arabia

Hadad

Alakilli

Rabah

et al. 2018

Saudi Journal of Biological Sciences

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Little is known about the prevalence of HBV genotypes/sub-genotypes in Jeddah province, although the hepatitis B virus (HBV) was identified as the most predominant type of hepatitis in Saudi Arabia. To characterize HBV genotypes/sub-genotypes, serum samples from 15 patients with chronic HBV were collected and subjected to gene amplification and sequence analysis. Phylogenetic analysis of the gene sequences revealed that 11 (48%) isolates belonged to HBV/D while 4 (18%) were associated with HBV/C. Notably, a HBV/D sub-genotype phylogenetic tree identified that eight current isolates (72%) belonged to HBV/D1, whereas three isolates (28%) appeared to be more closely related to HBV/D5, although they formed a novel cluster supported by a branch with 99% bootstrap value. Isolates belonging to D1 were grouped in one branch and seemed to be more closely related to various strains isolated from different countries. For further determination of whether the three current isolates belonged to HBV/D5 or represented a novel sub-genotype, HBV/DA, whole HBV genome sequences would be required. In the present study, we verified that HBV/D1 is the most prevalent HBV sub-genotype in Jeddah, and identified novel variant mutations suggesting that an additional sub-genotype designated HBV/DA should be proposed. Overall, the results of the present sequence analyses provide us with insights regarding the nucleotide differences between the present/D isolates identified in the populace of Jeddah, Saudi Arabia and those previously isolated worldwide. Additional studies with large numbers of subjects in other areas might lead to the discovery of the specific HBV strain genotypes or even additional new sub-genotypes that are circulating in Saudi Arabia.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Sequence analysis of sub-genotype D hepatitis B surface antigens isolated from Jeddah, Saudi Arabia

Hadad

Alakilli

Rabah

et al. 2018

Saudi Journal of Biological Sciences

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“…In the present study, eight isolates belonging to HBV/D (S18014, S140014, S20014, S140914, S02E12, S2312E, S07E and S10612E) are segregated with subgenotypes D1 reference isolates and inter distance equal 0.006 that was verified this result. Those in agreement with, recent studies reported the prevalence of HBV/D1 in regions of the Middle East through North Africa (Tran et al, 2014;El Hadad et al, 2013).…”

Section: Discussionsupporting

confidence: 91%

“…Recently, ten subgenotypes of HBV/D (D1 to D10) have been reported, each subgenotype showed distinct geographical clustering (Tran et al, 2014). Because little is known about the prevalence, distribution and sequences of HBV subgenotypes in Saudi Arabia, particularly Jeddah, no previous data was available (Al-Faleh et al, 1992.…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel D11 Hepatitis B Surface Antigen Subgenotype in Jeddah, Kingdom of Saudi Arabia

Hadad¹,

Alakilli²,

Rabah³

et al. 2015

Biotechnology

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A B S T R A C T Hepatitis B virus (HBV) infection remains to be a worldwide health problem. In Saudi Arabia, HBV is the most predominant type of hepatitis followed by hepatitis C and hepatitis A while little is known about the molecular epidemiology of the prevalence of HBV genotype/subgenotype particularly in Jeddah. Serum samples were collected from HBV chronic patients and subjected to HBsAg gene amplification. Sequencing and phylogenetic analysis of the entire HBsAg gene sequences revealed that 11 isolates belonged to HBV/D while 4 isolates were associated with HBV/C. Interestingly, HBV/D subgenotypes identified eight HBV/D present isolates belonged to HBV/D1 while three isolates showed a new cluster supporting by a branch with 99% bootstrap value and 4.3-5.8% nucleotide divergence over the entire HBsAg gene from other known subgenotypes D1 to D10, despite they were appearing more related to HBV/D5. The three strains of the new D subgenotype showed unique amino acid sequences consisting of Thr7non, 75Pro in the preS1 gene, 112Ile, 161Gly in the preS2 gene and 196Glu, 197Ala, 238Ser, 259Cys in the S gene. In addition to three amino acid residues in the S gene (373Ile, 374Ala and 381Thr) were specified S118S isolate. Subsequently, it have been verified that HBV/D1 is the most prevalent HBV subgenotype in Jeddah as well as we proposed a novel subgenotype designated HBV/D11. The identification of HBV/D11 novel subgenotypes in the present study suggested that further studies with a large number of subjects in previously examined and unexamined areas may lead to discovering new HBV strain genotypes and/or subgenotypes circulating in Saudi Arabia.

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“…6,7 Therefore, it is not surprising that several research efforts in the last decade have been directed to discover the different HBV sub-genotyping in several countries. [8][9][10][11] During HBV replication, the lack of proofreading ability of the reverse transcriptase leads to an error rate that is 10-fold higher than Mohammed El-Mowafy and Abdelaziz Elgaml contributed equally in this work.…”

mentioning

confidence: 99%

Molecular analysis of Hepatitis B virus sub‐genotypes and incidence of preS1/preS2 region mutations in HBV‐infected Egyptian patients from Mansoura

El‐Mowafy

Elgaml

El-Mesery

et al. 2017

Journal of Medical Virology

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Hepatitis B virus (HBV) is one of the major causes of viral hepatitis worldwide. Despite the prevalence of HBV infection in Egypt, few studies have focused on sub-genotyping of the virus. Moreover, no studies are available regarding the mutational analysis of the preS1/preS2 region of the viral genome, or its impact on hepatocellular carcinoma (HCC) development in Egypt. In this study, we have analyzed the sub-genotypes and incidence of mutations in the preS1/preS2 region of HBV present in HBV-infected patients, from Mansoura city (located in the center of Nile Delta region of Egypt), via partial sequencing of this specific region. Moreover, we have investigated the impact of these mutations on HCC development by measuring serum alpha fetoprotein (AFP) level and abdominal ultrasound examination of the HBV-infected patients. According to our results, all samples were genotype D in which sub-genotype D1 was predominant. In addition, the results revealed mutations in the preS1/preS2 region, which could result in either immature preS1 protein or completely inhibit the translation of the preS2 protein. However, there was no incidence of HCC development in patients infected with mutated HBV in the preS1/preS2 region. In summary, for the first time our work has proved the predominance of sub-genotype D1 among HBV-infected Egyptian patients in Mansoura city, Nile Delta region, Egypt, and incidence of mutations in the preS1/preS2 region of HBV genome. This current study opens up research opportunities to discuss the impact of HBV mutations on the development of HCC in Egypt.

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Novel quasi-subgenotype D2 of hepatitis B virus identified in Taiwanese aborigines

Cited by 7 publications

References 33 publications

Sequence analysis of sub-genotype D hepatitis B surface antigens isolated from Jeddah, Saudi Arabia

Sequence analysis of sub-genotype D hepatitis B surface antigens isolated from Jeddah, Saudi Arabia

Identification of Novel D11 Hepatitis B Surface Antigen Subgenotype in Jeddah, Kingdom of Saudi Arabia

Molecular analysis of Hepatitis B virus sub‐genotypes and incidence of preS1/preS2 region mutations in HBV‐infected Egyptian patients from Mansoura

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