Novel quartz crystal microbalance based biosensor for detection of oral epithelial cell–microparticle interaction in real-time

Elsom, Jacqueline; Lethem, Michael I.; Rees, Gareth D.; Hunter, A. Christy

doi:10.1016/j.bios.2007.11.020

Cited by 31 publications

(16 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Others have correlated the QCM signals and morphological changes with microscopy techniques but this has been done with cells that were extracted from their nutritive environment (i.e., fixed) (Elsom et al, 2008;Lord et al, 2009;Marx et al, 2001;Modin et al, 2006;Redepenning et al, 1993). Simultaneous microscopy observations and QCM-D signal detection would therefore act as a direct confirmation of frequency and energy dissipation changes.…”

Section: Introductionmentioning

confidence: 98%

Real-time QCM-D monitoring of cellular responses to different cytomorphic agents

Fatisson

Azari

Tufenkji

2011

Biosensors and Bioelectronics

View full text Add to dashboard Cite

Quartz crystal microbalance with dissipation monitoring (QCM-D) is used for real-time in situ detection of cytoskeletal changes in live primary endothelial cells in response to different cytomorphic agents; namely, the surfactant Triton-X 100 (TX-100) and bacterial lipopolysaccharide (LPS). Reproducible dissipation versus frequency (Df) plots provide unique signatures of the interactions between endothelial cells and cytomorphic agents. While the QCM-D response for TX-100 can be described in two steps (changes in the osmotic pressure of the medium prior to observing the expected cell lysis), LPS results in a different single-phase signal. A complementary analysis is carried out to evaluate the possible competitive effects of TX-100 and LPS through the QCM-D response to BAEC stress by analyzing the Df plots obtained. Experiments with non-toxic components (fibronectin or serum) produce a different QCM-D response than that observed for the toxic chemicals, suggesting the use of Df plot signatures for the possible differentiation between cytotoxic or non-cytotoxic effects. Observations obtained by QCM-D signals are confirmed by conducting fluorescence microscopy at the same time. Our results show that a fast (few minutes) sensing response can be obtained in situ and in real-time. The conclusions from this study suggest that QCM-D can potentially be used in biodetection for applications in drug screening tests and diagnosis.

show abstract

Section: Introductionmentioning

confidence: 98%

Real-time QCM-D monitoring of cellular responses to different cytomorphic agents

Fatisson

Azari

Tufenkji

2011

Biosensors and Bioelectronics

View full text Add to dashboard Cite

show abstract

“…These include autoimmune disease, transplant rejection, leukaemia and lymphoma [171]. At present many treatment regimens fall short in effectiveness and/or promote toxicity due to Permission from the authors given for use of (A) and (C) [141].…”

Section: Self-nanoemulsifying Drug Delivery Systems (Snedds)mentioning

confidence: 99%

“…5). This system allowed monitoring in real time using a quartz crystal microbalance [141,142]. Nanoparticles have been internalized by both human oral explants as well as human oral squamous cell carcinoma in a 10-100 nm range [139].…”

Section: Polymeric Nanoparticles and The Oral Cavitymentioning

confidence: 99%

Polymeric particulate technologies for oral drug delivery and targeting: A pathophysiological perspective

et al. 2012

Self Cite

View full text Add to dashboard Cite

The oral route for delivery of pharmaceuticals is the most widely used and accepted. Nanoparticles and microparticles are increasingly being applied within this arena to optimize drug targeting and bioavailability. Frequently the carrier systems used are either constructed from or contain polymeric materials. Examples of these nanocarriers include polymeric nanoparticles, solid lipid nanocarriers, self-nanoemulsifying drug delivery systems and nanocrystals. It is the purpose of this review to describe these cutting edge technologies and specifically focus on the interaction and fate of these polymers within the gastrointestinal system.

show abstract

“…For optimal reaction parameters of deposition, the RF plasma instrument used 100 mTorr of monomer with a plasma power of 30 W. This study tested the change on the QCM chip surface, using HMDSZ reaction times of 1, 3, 5, 7, and 10 min to form a protective film. After plasma deposition, the silver electrode QCM surface was made hydrophilic by oxygen plasma photo-induced polymerization using the traditional process (Chen et al, 2007;Elsom et al, 2008). This study presents three methods to increase surface hydrophilicity.…”

Section: Preparation Of Plasma-polymerized Film and Photo-induced Polmentioning

confidence: 99%

QCM as Cell-Based Biosensor

Yan¹,

Lee²,

Chou³

2012

Chemical Biology

View full text Add to dashboard Cite

Novel quartz crystal microbalance based biosensor for detection of oral epithelial cell–microparticle interaction in real-time

Cited by 31 publications

References 48 publications

Real-time QCM-D monitoring of cellular responses to different cytomorphic agents

Real-time QCM-D monitoring of cellular responses to different cytomorphic agents

Polymeric particulate technologies for oral drug delivery and targeting: A pathophysiological perspective

QCM as Cell-Based Biosensor

Contact Info

Product

Resources

About