Novel QTL associated with the Fusarium head blight resistance in Truman soft red winter wheat

Islam, Sariful; Brown-Guedira, Gina; Sanford, David A. Van; Ohm, H. W.; Dong, Yanhong; McKendry, Anne L.

doi:10.1007/s10681-015-1550-9

Cited by 26 publications

(33 citation statements)

References 73 publications

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“…Although the mechanisms behind different types of FHB resistances may differ, we found that several of the identified QTL regions were associated with more than one type of resistance, which has also been reported in previous FHB studies with Ernie (Liu et al, 2007;Abate et al, 2008;Liu et al, 2013) and Truman (Islam et al, 2016). Twelve of these QTL were significant in more than one environment and/or for different resistance traits and were therefore considered reproducible.…”

Section: Discussionsupporting

confidence: 83%

“…nc-6A.3 from the present study overlapped with the QTL regions Q fhb.nc-1A, Q fhb.nc-4A, and Q fhb.nc-6A identified in the NC-Neuse ´ AGS 2000 population (Petersen et al, 2016) (Fig. nc-2B.1 and Q fhb.nc-3B.2 from the present study overlapped with QTL regions identified in the Truman ´ MO 94-317 study (Islam et al, 2016) (Fig. NC-Neuse alleles conferred resistance at all three QTL in both studies.…”

Section: Linkage Map For Bess ´ Nc-neuse Doubled Haploid Populationsupporting

confidence: 75%

“…In the present study, Bess alleles were associated with resistance at both QTL, and Truman alleles were associated with resistance in the Truman ´ MO 94-317 mapping study (Islam et al, 2016). In the present study, Bess alleles were associated with resistance at both QTL, and Truman alleles were associated with resistance in the Truman ´ MO 94-317 mapping study (Islam et al, 2016).…”

Section: Discussionsupporting

confidence: 61%

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Validation of Fusarium Head Blight Resistance QTL in US Winter Wheat

et al. 2017

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Fusarium head blight (FHB), primarily caused by Fusarium graminearum Schwabe [telemorph: Gibberella zeae Schw. (Petch)], can significantly reduce the grain quality of wheat (Triticum aestivum L.) due to mycotoxin contamination. Two US soft red winter wheat cultivars, Bess and NC‐Neuse, have moderate resistance to FHB. The objective of this study was to validate genomic regions associated with FHB resistance identified in previous studies involving NC‐Neuse and the cultivar Truman, a full‐sib of Bess. A total of 98 doubled haploid lines derived from the cross Bess × NC‐Neuse were evaluated in inoculated, mist‐irrigated field nurseries. The lines were evaluated for FHB incidence, severity, Fusarium‐damaged kernels, and deoxynivalenol content in seven environments between 2011 and 2014. A 3338‐cM linkage map was developed based on 4014 simple sequence repeat and single nucleotide polymorphism markers. Twelve quantitative trait loci (QTL) associated with FHB resistance were identified. NC‐Neuse alleles provided resistance at QTL on five chromosomes and Bess alleles provided resistance at QTL on five other chromosomes. Alignment of linkage maps revealed that five of these QTL were overlapping with previously identified regions. Quantitative trait loci on chromosomes 1A, 4A, and 6A identified in this study overlapped with QTL regions identified in NC‐Neuse, and QTL identified on chromosomes 2B and 3B overlapped with QTL regions identified in Truman. A preliminary test using Kompetitive Allele‐Specific polymerase chain reaction assays on recent Uniform Southern Winter Wheat Scab Nursery entries showed that the assays developed for Qfhb.nc‐2B.1 may be good candidates for use in marker‐assisted selection.

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Section: Discussionsupporting

confidence: 83%

Section: Linkage Map For Bess ´ Nc-neuse Doubled Haploid Populationsupporting

confidence: 75%

Section: Discussionsupporting

confidence: 61%

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Validation of Fusarium Head Blight Resistance QTL in US Winter Wheat

et al. 2017

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“…Fusarium head blight resistance QTL have been identified on all 21 chromosomes (Buerstmayr Liu et al, 2009;Cai et al, 2016). The cultivar Truman was studied by Islam et al (2016), and QTLs associated with FDK and DON were found on chromosomes 2A, 2D, and 3B. Our GWAS for FDK and DON identified SNPs on chromosomes 5B, 6B, and 4A.…”

Section: Genome-wide Association Studymentioning

confidence: 79%

“…The QTL analyzed in the population were Fhb1 , height ( Rht‐B1 and Rht‐D1 ), vernalization ( Vrn‐A1 , Vrn‐B1 , and Vrn‐D3 ), and photoperiod ( Ppd‐A1 , Ppd‐B1 , and Ppd‐D1 ) genes. These QTL were chosen for analysis because they are either important scab resistance genes ( Fhb1 ) or fundamental growth and development genes that have been implicated in FHB studies (e.g., Ppd‐D1 ; Islam et al, 2016). Using the average of each trait, the population was classified for each allelic form, and levels of PH, HD, FHB rating, FDK, and DON were calculated for each QTL.…”

Section: Resultsmentioning

confidence: 99%

GWAS for Fusarium Head Blight Traits in a Soft Red Winter Wheat Mapping Panel

2019

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Fusarium head blight (FHB) is an important disease of wheat (Triticum aestivum L.) that has caused billions of dollars in losses in recent decades. Although a massive breeding effort has been undertaken on multiple continents, there are no wheat cultivars with immunity to the disease. Resistance is conditioned by multiple loci and is further complicated by the role of the environment in expression of the disease phenotype. The objectives of our study were (i) to evaluate the phenotypic response to FHB in a large, diverse soft red winter wheat mapping panel; and (ii) to identify promising quantitative trait loci (QTL) associated with FHB resistance based on a genome‐wide association study (GWAS). We evaluated the mapping panel in 2014–2015 and 2015–2016 in an irrigated, inoculated scab nursery near Lexington, KY. Traits evaluated were heading date, plant height, FHB rating, severity, incidence, index, Fusarium‐damaged kernels (FDK), and deoxynivalenol (DON). There were significant (p < 0.05) differences among genotypes for all traits measured. The GWAS (based on 2‐yr entry means) identified 16 significant (p < 0.001) single nucleotide polymorphisms (SNPs) associated with disease traits on multiple chromosomes. Single nucleotide polymorphism association ranged from −2.14 to 4.01% of the mean of a given trait. We detected SNPs associated with FDK and DON on chromosomes 4A, 5B and 6B, and these SNPs decreased DON levels by 1.5, 2.1 and 3.2 mg kg−1, respectively. Our study demonstrated that even small‐effect QTL can potentially decrease disease levels and thus be useful in breeding programs.

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Genome‐wide analysis and prediction of Fusarium head blight resistance in soft red winter wheat

et al. 2020

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Fusarium head blight (FHB) is a disease in wheat (Triticum aestivum L.) caused by the fungal pathogen Fusarium graminearum Schwabe. Fusarium head blight poses potential economic losses and health risks due to the accumulation of the mycotoxin deoxynivalenol (DON) on infected seed heads. The objectives of this study were to identify novel FHB resistance loci using a genome‐wide association study (GWAS) approach and to evaluate two genomic selection (GS) approaches to improve prediction accuracies for FHB traits in a population of 354 soft red winter wheat (SRWW) genotypes. The GS approaches included GS+GWAS, where markers associated with a trait were used as fixed effects, and multivariate GS (MVGS), where correlated traits were used as covariates. The population was evaluated in FHB nurseries in Fayetteville and Newport, AR, and Winnsboro, LA, from 2014 to 2017. Genotypes were phenotyped for DON, Fusarium‐damaged kernels (FDK), incidence (INC), and severity (SEV). Forty‐two single nucleotide polymorphism (SNP) markers were significantly (false discovery rate, q [FDRq] ≤ .10) associated with resistance traits across 17 chromosomes. Ten significant SNPs were identified for DON, notably on chromosomes 2BL and 3BL. Eleven were identified for FDK, notably on chromosomes 4BL, 3AL, 1BL, 5BL, and 5DL. Nine were identified for INC, notably on chromosomes 2BS, 2BL, 7BL, 5DL, 6AS, and 5DS. Twelve were identified for SEV, notably on chromosomes 3BL, 4AL, and 4BL. The naïve GS models significantly outperformed the GS+GWAS model for all traits, whereas MVGS models significantly outperformed the naïve GS models for all traits. Results from this study will facilitate the development of SRWW cultivars with improved FHB resistance.

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Novel QTL associated with the Fusarium head blight resistance in Truman soft red winter wheat

Cited by 26 publications

References 73 publications

Validation of Fusarium Head Blight Resistance QTL in US Winter Wheat

Validation of Fusarium Head Blight Resistance QTL in US Winter Wheat

GWAS for Fusarium Head Blight Traits in a Soft Red Winter Wheat Mapping Panel

Genome‐wide analysis and prediction of Fusarium head blight resistance in soft red winter wheat

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