Novel properties of antimicrobial peptide anoplin

Jindřichová, Barbora; Burketová, Lenka; Novotná, Zuzana

doi:10.1016/j.bbrc.2014.01.097

Cited by 18 publications

(16 citation statements)

References 24 publications

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“…Similar antimicrobial activity is also reported in cationic peptides polybia-MPI [35], anoplin [35] [45] and their synthetic analogues [35] [46]. Anoplin shows membrane anchoring through a lipophilic amino acid derivative and slight changes in its structure shows improvement in biological activity [47].…”

Section: Discussionsupporting

confidence: 74%

Antimicrobial Activity of Purified Toxins from Yellow Wasp <i>Polistes flavus</i> (Vespidae) against Certain Bacteria and Fungi

Prajapati¹,

Upadhyay²

2016

JBM

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Yellow Wasp Polistes flavus venom toxins were isolated and purified on a Sepharose CL-6B 200 column. Purified proteins were investigated for its antibacterial and antifungal activity against 13 infectious microbial pathogens. Paper disc diffusion and serial micro-dilution assays were performed for the determination of inhibition zone (DIZ) diameters and minimal inhibitory concentration, respectively. Triton X-100 (0.1%) proved to be a good solubilizing agent for toxin/proteins. Higher protein solubilization was observed in the supernatant than in the residue, except TCA (tri-chloroacetic acid). The elution pattern of purified and homogenized sting glands exhibited two major peaks at 280 nm in fraction No. 41-61 and 81-101. The total yield of protein was 69.21% and specific activity was determined in each fraction. Molecular weights in protein fractions were ranging from 6-70 kD. MIC (Minimum Inhibitory Concentration) values were 12.3 μg/ml against K. pneumonia 12.3 μg/ml against E. coli and L. acidophilus, 24.6 μg/ml against B. cereus; 49.24 μg/ml against S. aureus and M. luteus. By agar disc diffusion method, the diameter of inhibition zones in mm in presence of yellow wasp toxins is at a concentration range of 98.56-6.9 μg/ml E. coli 18.36 ± 0.14, Bacillus cereus 14.566 ± 0.21, L. acidophilus 18.10 ± 0.21, Micrococcus luteus 18.76 ± 0.19, S. aeurus 17.36 ± 0.43, Klebsiella pneuminiae 19.56 ± 0.21, Salmonella typhi 19.96 ± 0.31, Vibrio cholera 21.83 ± 0.22, Pseudomonas aeruginosa 22.90 ± 0.09, Aspergillus niger 21.66 ± 0.16, Candida albicans 23.33 ± 0.26, Rhizopus stolonifer 24.96 ± 0.16 respectively. The antibacterial and antifungal activity of venom toxin may be due to action on cell membrane, its destruction and cell lysis. The wasp toxins may be used as strong biological agents to control microbes.

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Section: Discussionsupporting

confidence: 74%

Antimicrobial Activity of Purified Toxins from Yellow Wasp <i>Polistes flavus</i> (Vespidae) against Certain Bacteria and Fungi

Prajapati¹,

Upadhyay²

2016

JBM

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“…Amphipathic linear cationic α-helical peptides anoplin and decoralin, found in a Pompilidae wasp A. samariensis and an Eumeninae wasp Oreumenes decoratus , respectively, commonly have MCD and antimicrobial activities [ 27 , 28 , 120 ]. Anoplin has hemolytic activity as well [ 121 ].…”

Section: Venom Peptidesmentioning

confidence: 99%

Differential Properties of Venom Peptides and Proteins in Solitary vs. Social Hunting Wasps

Lee

Baek

Yoon

2016

Toxins

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The primary functions of venoms from solitary and social wasps are different. Whereas most solitary wasps sting their prey to paralyze and preserve it, without killing, as the provisions for their progeny, social wasps usually sting to defend their colonies from vertebrate predators. Such distinctive venom properties of solitary and social wasps suggest that the main venom components are likely to be different depending on the wasps’ sociality. The present paper reviews venom components and properties of the Aculeata hunting wasps, with a particular emphasis on the comparative aspects of venom compositions and properties between solitary and social wasps. Common components in both solitary and social wasp venoms include hyaluronidase, phospholipase A2, metalloendopeptidase, etc. Although it has been expected that more diverse bioactive components with the functions of prey inactivation and physiology manipulation are present in solitary wasps, available studies on venom compositions of solitary wasps are simply too scarce to generalize this notion. Nevertheless, some neurotoxic peptides (e.g., pompilidotoxin and dendrotoxin-like peptide) and proteins (e.g., insulin-like peptide binding protein) appear to be specific to solitary wasp venom. In contrast, several proteins, such as venom allergen 5 protein, venom acid phosphatase, and various phospholipases, appear to be relatively more specific to social wasp venom. Finally, putative functions of main venom components and their application are also discussed.

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“…As indicated in Figure 3 A, the modification of the MR peptide resulted in lower toxicity to E. coli BL21 than the MR peptide itself. To confirm that the bacteria were killed after being treated with peptides, 20 μL specimens from the treated groups were added into 2 mL fresh Muller-Hinton broth (MHB) medium and then cultured at 37 °C for 20 h. As shown in Figure 3 B, there were no viable bacteria which existed in the medium with the similar pattern as the positive anoplin (GL) peptide control group, which holds a bactericidal activity [ 17 , 18 , 19 ]. From the killing kinetic experiments, it was further found that MR at 5.34 mM and a-MR at 6.47 mM were able to kill the bacteria (3log 10 killed) within 10 min while the f-MR at 8 mM killed the bacteria within 160 min ( Figure 3 C).…”

Section: Resultsmentioning

confidence: 99%

The Novel Property of Heptapeptide of Microcin C7 in Affecting the Cell Growth of Escherichia coli

et al. 2017

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Microcin C7 (McC), widely distributed in enterobacteria, is a promising antibiotic against antibiotic resistance. Previous studies have demonstrated that the heptapeptide of McC is only responsible for recognizing the inner membrane transporter YejABEF to deliver McC into microbial cells, but lacks the capacity for inhibiting microbial cell growth. In this study, the effect of the heptapeptide (MR) and two analogues, N-formylated heptapeptide (f-MR) and N-aceylated heptapeptide (a-MR), on microbial cell growth were examined. It is surprising to find that MR not only inhibits the activity of intracellular β-galactosidase, respiratory chain dehydrogenases, and 6-phosphogluconate dehydrogenases (6PGDH), but it is also able to inhibit Escherichia coli growth, and eventually leads to cell death at the lethal concentration of 5.34 mM within 10 min. The modification of MR results in a slight increase in the lethal concentration. Cell membrane integrity at the lethal concentration confirms that MR undergoes the inhibition effect, but not by destroying the cell membrane integrity. The novel property of MR provides a new insight into the Trojan horse strategy of McC and opens a new route for antibiotics design.

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Novel properties of antimicrobial peptide anoplin

Cited by 18 publications

References 24 publications

Antimicrobial Activity of Purified Toxins from Yellow Wasp <i>Polistes flavus</i> (Vespidae) against Certain Bacteria and Fungi

Antimicrobial Activity of Purified Toxins from Yellow Wasp <i>Polistes flavus</i> (Vespidae) against Certain Bacteria and Fungi

Differential Properties of Venom Peptides and Proteins in Solitary vs. Social Hunting Wasps

The Novel Property of Heptapeptide of Microcin C7 in Affecting the Cell Growth of Escherichia coli

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Novel properties of antimicrobial peptide anoplin

Cited by 18 publications

References 24 publications

Antimicrobial Activity of Purified Toxins from Yellow Wasp &lt;i&gt;Polistes flavus&lt;/i&gt; (Vespidae) against Certain Bacteria and Fungi

Antimicrobial Activity of Purified Toxins from Yellow Wasp &lt;i&gt;Polistes flavus&lt;/i&gt; (Vespidae) against Certain Bacteria and Fungi

Differential Properties of Venom Peptides and Proteins in Solitary vs. Social Hunting Wasps

The Novel Property of Heptapeptide of Microcin C7 in Affecting the Cell Growth of Escherichia coli

Contact Info

Product

Resources

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Antimicrobial Activity of Purified Toxins from Yellow Wasp <i>Polistes flavus</i> (Vespidae) against Certain Bacteria and Fungi

Antimicrobial Activity of Purified Toxins from Yellow Wasp <i>Polistes flavus</i> (Vespidae) against Certain Bacteria and Fungi