Novel Production of Isochainin by a Strain of Streptomyces sp. Isolated from Rhizosphere Soil of the Indigenous Moroccan Plant Argania Spinosa L

Abstract

An actinomycete strain (designated Ap1) isolated from the rhizosphere soil of Argania spinosa L. strongly inhibited the growth of two plant pathogens: Fusarium oxysporum f.sp. albedinis and Verticillium dahliae. The spore morphology suggested that the Ap1 strain belonged to the genus Streptomyces. The antifungal compound produced by Ap1 was purified by HPLC and identified as the polyene macrolide, isochainin, by NMR and mass spectroscopy. Ap1 showed normal biosynthesis of isochainin in comparison with S. cellulosae ATTC 12625, in which precursordirected biosynthesis by feeding ethyl (Z)-16-phenylhexadec-9-enoate to the culture medium is required. In addition, Streptomyces sp. strain Ap1 produces isochainin with a 6.5-fold higher concentration than Streptomyces cellulosae ATTC 12625.

Streptomyces marokkonensis sp. nov., isolated from rhizosphere soil of Argania spinosa L.

“…In this paper, strain Ap1 T , with antagonistic activity towards some phytopathogens (Bouizgarne et al, 2006), was shown to present a colonial morphology and cell-wall chemistry consistent with its assignment to the genus Streptomyces. In the present investigation, strain Ap1…”

“…T was isolated from a soil sample collected from the rhizosphere of an argan tree (Argania spinosa L.) as described by Bouizgarne et al (2006). The isolate was grown on oatmeal agar [International Streptomyces Project (ISP) medium 3; Kuster, 1959] at 28 u C for 2 weeks and stored at room temperature.…”

Evaluation of Streptomyces spp. for biological control of Sclerotium root and stem rot and Ralstonia wilt of chili pepper

“…on plant pathogens, for example: S. rochei on Phytopthora root rot of pepper (Ezziyyani et al 2007); S. griseoviridis on Fusarium root rot and wilt of tomato (Minuto et al 2006); S. platensis on R. solani leaf blight/seedling blight of rice (Wan et al 2008); S. hygroscopicus on Colletotrichum gloeosprioides anthacnose of several crops (Prapagdee et al 2008 the ability of Streptomyces to produce antifungal and antibacterial substances was supported by many reports and the Streptomyces are also a major source of innumerable antibiotic agents (Mahadevan and Crawford 1997;Neeno et al 2001;Lee and Hwang 2002). The success attained by using Streptomyces as a biocontrol agent of plant diseases has been reported in studies such as Bouizgarne et al (2006) who found that the Streptomyces sp. Ap1 produced isochainin and strongly inhibited the growth of two pathogens Fusarium oxysporum f. sp.…”

Assessing the Diversity and Composition of Bacterial Communities across a Wetland, Transition, Upland Gradient in Macon County Alabama

“…Another characteristic of Actinomycetes that lead to such an impact on variation in composition is the ability of a good deal of them to produce antimicrobial compounds, thus allowing them to impact the community around them [59][60][61][62]. Actinomycetes have also been described as increasing in abundance in subsurface [63], which gives validity to the observation of the constantly eroded soil surface of the TSS, possibly allowing subsurface microbiota to become predominant in such an environment.…”