Novel production of biodispersant by <i>Serratia marcescens</i> UCP 1549 in solid-state fermentation and application for oil spill bioremediation

Almeida²,

Silva³

et al. 2021

Processes

Among the innovative technologies utilized for the treatment of contaminated soils, the use of green surfactants appears to be a biocompatible, efficient, and attractive alternative, since the cleaning processes that normally use synthetic surfactants as additives cause other problems due to toxicity and the accumulation of by-products. Three green surfactants, i.e., two biobased (biobased 1 and biobased 2) surfactants produced by chemical synthesis and a microbial surfactant produced from the yeast Starmerella bombicola ATCC 22214, were used as soil remediation agents and compared to a synthetic surfactant (Tween 80). The three surfactants were tested for their ability to emulsify, disperse, and remove different hydrophobic contaminants. The biosurfactant, which was able to reduce the water surface tension to 32.30 mN/m at a critical micelle concentration of 0.65 g/L, was then used to prepare a commercial formulation that showed lower toxicity to the tested environmental bioindicators and lower dispersion capacity than the biobased surfactants. All the green surfactants showed great emulsification capacity, especially against motor oil and petroleum. Therefore, their potential to remove motor oil adsorbed on different types of soils (sandy, silty, and clay soil and beach sand) was investigated either in kinetic (flasks) or static (packed columns) experiments. The commercial biosurfactant formulation showed excellent effectiveness in removing motor oil, especially from contaminated sandy soil (80.0 ± 0.46%) and beach sand (65.0 ± 0.14%) under static conditions, while, in the kinetic experiments, the commercial biosurfactant and the biobased 2 surfactant were able to remove motor oil from all the contaminated soils tested more effectively than the biobased 1 surfactant. Finally, the S. bombicola commercial biosurfactant was evaluated as a soil bioremediation agent. In degradation experiments carried out on motor oil-contaminated soils enriched with sugarcane molasses, oil degradation yield in the sandy soil reached almost 90% after 60 days in the presence of the commercial biosurfactant, while it did not exceed 20% in the presence of only S. bombicola cells. These results promise to contribute to the development of green technologies for the treatment of hydrophobic pollutants with economic gains for the oil industries.

Section: Toxicity Of the Surfactants To Artemia Salinamentioning

confidence: 95%

Application of Green Surfactants in the Remediation of Soils Contaminated by Hydrocarbons

Almeida²,

Silva³

et al. 2021

Processes

“…Particularly important considering that synthetic dyes are employed in diverse industries such as food, textile, plastics, food, and pharmaceutical (Falade and Ekundayo, 2021). Bioremediation strategies using S. marcescens have been developed as a promising alternative for marine ecosystems impacted by petroderivatives (dos Santos et al, 2021).…”

Section: Genes Involved In Antimicrobial Activities or Important Enzymes For Industrymentioning

confidence: 99%

“…For example, marine S. marcescens demonstrated antioxidant and antibacterial activity against some Gram-positive and Gram-negative bacteria (Gangadharan et al, 2020). Moreover, oil spills in aquatic ecosystems can be bioremediated by a biodispersant produced by S. marcescens characterized by low toxicity, high biodegradability, and good ecological acceptability (dos Santos et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genomic analysis and plant growth-promoting potential of a Serratia marcescens isolated from food

Miller

Pereira

et al. 2022

RSD

A genomic analysis of the potential application of a Serratia marcescens strain in the plant-growth promotion. We performed whole-genome sequencing of Serratia marcescens isolated from a Minas Frescal Cheese. The genomic repertoire revealed a bacterium of agricultural and biotechnological interest. In the plant-growth promotion traits, we highlight genes encoding proteins possibly responsible for the biosynthesis of phytohormone indole acetic acid, organic compounds that act in iron uptake, and the Phosphate solubilization system. Genes encoding for enzymes like the versatile L-asparaginase stimulates the development of seeds and grains and can benefit the food industry due to a mitigation effect on acrylamide and notably, has medical applications as a chemotherapeutic agent or is applicable by its antimicrobial and anti-inflammatory properties. Moreover, functional diversity of genes encoding for resistance to different metals and metabolism of xenobiotics genes can be found in this strain, reinforcing its biotechnological potential. The versatile enzymes that can be produced by S. marcescens benefit the food, pharmaceutical, textile, agronomic, and cosmetic industries. The relevant genetic systems of S. marcescens described here may be used to promote plant growth and health and improve the environment. To the best of our knowledge, this is the first genome sequence report on S. marcescens isolated from cheese, with potential application as promoting plant growth and providing a baseline for future genomic studies on the development of this species.

“…Assim, nas atividades que envolvem a utilização do petróleo ou de seus derivados, em processos que vão desde a extração e refino até a distribuição dos mesmos, a ocorrência de derrames acidentais constitui um fator de relevante preocupação, considerando que esses compostos apresentam propriedades tóxicas, mutagênicas e carcinogênicas aos seres vivos (Ribeiro, 2014;dos Santos, et al, 2021). Neste sentido, um dos maiores desastres socioambientais já registrados no Brasil aconteceu em agosto de 2019, provocado por um vazamento de petróleo de origem ainda desconhecida que se alastrou por mais de 70% dos 3.300 quilômetros do litoral Nordeste do país (Araújo, et al, 2020;Fukushima, 2022).…”

Section: Introductionunclassified

Bioprospecção de fungos filamentosos isolados de sedimentos de mangue do estado de Pernambuco para biodegradação de petroderivados

Mendonça²,

Costa³

et al. 2022

RSD

As atividades que envolvem a utilização do petróleo e seus derivados têm sido motivo de constante preocupação pelos danos causados aos ecossistemas, devido à elevada toxicidade e difícil degradação destes compostos. Consequentemente, diversos métodos de descontaminação vêm sendo desenvolvidos, destacando-se dentre eles a biorremediação, uma técnica ecologicamente viável que visa à aplicação de micro-organismos e/ou seus produtos para remover ou degradar derivados do petróleo e minimizar os efeitos nocivos nas áreas impactadas. Neste sentido, a utilização de micro-organismos isolados do próprio ecossistema constitui uma estratégia promissória, considerando que já possuem mecanismos de resistência a essas condições ambientais adversas. Assim, o objetivo deste trabalho foi avaliar o potencial de biodegradação de petroderivados de fungos filamentosos isolados de sedimentos de mangue do Estado de Pernambuco, contaminados com o derramamento ocorrido em agosto de 2019 na costa do Nordeste brasileiro. Para isso, quatro isolados fúngicos pertencentes aos gêneros Aspergillus sp., Penicillium sp., Talaromyces sp. e Trichoderma sp. foram submetidos a aclimatação em meio Ágar Sabouraud contendo 1-20% de óleo de motor queimado, constatando-se o crescimento em todas as concentrações após 72 h. Em seguida, os micro-organismos aclimatados a 20% foram selecionados para o ensaio de biodegradação de diesel e querosene, no meio Bushnell Haas contendo o indicador redox 2,6-diclorofenol-indofenol (DCPIP), verificando-se resultados acima de 75% após 72 h. Contudo, Aspergillus sp. demonstrou o maior potencial, uma vez que alcançou 88,4% de biodegradação de ambos petroderivados. Assim, sugere-se a utilização deste micro-organismo promissor em processos de biorremediação de ecossistemas impactados por petróleo e derivados.