Novel primer sequences for polymerase chain reaction-based detection of Trypanosoma brucei gambiense.

Radwanska, Magdalena; Claes, Filip; Magez, Stefan; Magnus, E; Pérez‐Morga, David; Pays, Étienne; Büscher, Philippe

doi:10.4269/ajtmh.2002.67.289

Cited by 141 publications

(145 citation statements)

References 54 publications

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“…The recently developed T. b. gambiense-specific PCR test based on the use of the T. gambiense-specific glycoprotein gene can also facilitate the correct diagnosis of both parasites that cause sleeping sickness. 22,23 Since the VSG gene sequences of T. brucei show high mutation rates, this can hamper their use in diagnostic and epidemiologic studies. 24 Indeed, the use of the AnTat 11.17 and LiTat 1.3 (CATT antigen) VSG sequences in identifying T. b. gambiense showed that these genes and their products, although present in most strains, may be missing in some isolates from northwestern Uganda and Cameroon.…”

Section: Discussionmentioning

confidence: 99%

The serum resistance-associated gene as a diagnostic tool for the detection of Trypanosoma brucei rhodesiense.

Radwanska

Chamekh²,

Vanhamme³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

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Abstract. In the search for new diagnostic methods that would distinguish Trypanosoma brucei rhodesiense from T. b. brucei and T. b. gambiense, we have developed two polymerase chain reaction (PCR) primer sets. The first primer set was derived from the serum resistance−associated (SRA) gene of T. b. rhodesiense that confers resistance to lysis by normal human serum (NHS). The specificity of the SRA-based PCR was tested on 97 different trypanosome populations originating from various taxonomic groups, host species, and geographic regions. Only one of 25 T. b. rhodesiense samples was negative in this PCR, and none of 72 other samples were positive in this assay. Interestingly, a reference T. brucei strain (TREU927/4) currently used for genome sequencing was negative for the SRA gene; however, this strain was resistant to lysis by NHS. The second primer set was derived from a specific variant surface glycoprotein (VSG) expression site where the SRA gene is expressed (R-ES). This primer set identified the strain as T. b. rhodesiense in 17 of 17 SRA gene-positive strains in which it was tested. These data strongly suggest that expression of the SRA gene is generally involved in resistance to lysis by NHS in T. b. rhodesiense strains.

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Section: Discussionmentioning

confidence: 99%

The serum resistance-associated gene as a diagnostic tool for the detection of Trypanosoma brucei rhodesiense.

Radwanska

Chamekh²,

Vanhamme³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

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140

124

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“…These are PCR based, targeting the serum resistanceassociated (SRA) gene that is specific for T. b. rhodesiense (4,6,14) or the T. b. gambiense surface glycoprotein (TgSGP) that is specific for that subspecies (2,15). The need for specialized equipment and a constant supply of electricity has hindered integration of those techniques into the diagnostic algorithm.…”

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confidence: 99%

Comparative Detection of Trypanosomal DNA by Loop-Mediated Isothermal Amplification and PCR from Flinders Technology Associates Cards Spotted with Patient Blood

et al. 2010

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“…gambiense, respectively; T.b. brucei has neither (3,4). Over 20 species and subspecies of tsetse flies (Glossina spp.)…”

Section: Introductionmentioning

confidence: 99%

Kinetoplastids: related protozoan pathogens, different diseases

Stuart¹,

Brun²,

Croft³

et al. 2008

J. Clin. Invest.

490

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Kinetoplastids are a group of flagellated protozoans that include the species Trypanosoma and Leishmania, which are human pathogens with devastating health and economic effects. The sequencing of the genomes of some of these species has highlighted their genetic relatedness and underlined differences in the diseases that they cause. As we discuss in this Review, steady progress using a combination of molecular, genetic, immunologic, and clinical approaches has substantially increased understanding of these pathogens and important aspects of the diseases that they cause. Consequently, the paths for developing additional measures to control these "neglected diseases" are becoming increasingly clear, and we believe that the opportunities for developing the drugs, diagnostics, vaccines, and other tools necessary to expand the armamentarium to combat these diseases have never been better.

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Novel primer sequences for polymerase chain reaction-based detection of Trypanosoma brucei gambiense.

Cited by 141 publications

References 54 publications

The serum resistance-associated gene as a diagnostic tool for the detection of Trypanosoma brucei rhodesiense.

The serum resistance-associated gene as a diagnostic tool for the detection of Trypanosoma brucei rhodesiense.

Comparative Detection of Trypanosomal DNA by Loop-Mediated Isothermal Amplification and PCR from Flinders Technology Associates Cards Spotted with Patient Blood

Kinetoplastids: related protozoan pathogens, different diseases

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