Novel polymorphisms in UTR and coding region of inducible heat shock protein 70.1 gene in tropically adapted Indian zebu cattle (Bos indicus) and riverine buffalo (Bubalus bubalis)

Genetic diversity of the 3ꞌ and 5ꞌ untranslated regions of the <i>HSP70.1</i> gene between native Turkish and Holstein Friesian cattle breeds

“…Sequencing of the two fragments of the HSP70.1-3ꞌ UTR and HSP70.1-5ꞌ UTR revealed that these regions are more variable among Turkish native cattle breeds than other cattle breeds that have previously been investigated (Grosz et al, 1994;Adamowicz et al, 2005;Basirió et al, 2011;Sodhi et al, 2013). In the less polymorphic region HSP70.1-3ꞌUTR, 13 SNPs and one indel were detected (Table 3).…”

“…Two SNPs were reported at nucleotides 63 and 2154 of the HSP70.1-3ꞌ UTR in the European origin cattle breeds at low frequencies by Grosz et al, (1994) and Adamowicz et al, (2005). On the other hand, Basirió et al, (2011) and Sodhi et al, (2013) found that the 253 bp region of the HSP70.1-3ꞌ UTR was monomorphic in both the Bos taurus and Bos indicus cattle breeds. These results are concordant with findings in the current study.…”

“…In addition to this functional importance, close proximity to the major histocompatibility complex genes highlights the HSP70.1 gene as a powerful candidate marker for health, reproduction and productive traits (Wurst et al, 1989). For these reasons, studies of polymorphisms in this gene and resultant phenotypic traits in both livestock and human beings have increased (Wu et al, 2004;Basiricó et al, 2011;Sodhi et al, 2013;Xiong et al, 2013).…”

“…While the 5' UTR may affect the expression level of the transcript, the 3' UTR is thought to affect mRNA stability (Basiricó et al, 2011;Sodhi et al, 2013). Various studies have been carried out to detect mutations in these gene regions, and interest in the relationships between these mutations and reproductive traits has grown (Grosz et al, 1994;Schwerin et al, 2003;Adamowicz et al, 2005;Banks, 2007a;Rosenkrans et al, 2010;Basiricó et al, 2011;Sodhi et al, 2013;Xiong et al, 2013;Deb et al, 2013). It was also reported that mutations may occur at promoter regions of HSP70.1 and may negatively affect spermatogenesis, embryonic mortality, and pregnancy (Rivera & Hansen, 2001;Hansen et al, 2001;Huang et al, 2002).…”

Genetic diversity analysis of the thyroglobulin gene promoter in buffalo and other bovines

“…Four polymorphic sites were common across cattle and buffalo (121G4T, 166C 4T, 291C 4T and 442C4T). The allele and genotype frequencies for each of the identified SNPs were estimated across cattle and buffalo breeds as reported by earlier workers in candidate gene polymorphism across breeds/populations (Yoon et al, 2005;Hou et al, 2011;Sodhi et al, 2013). The frequency of C allele at position 378, reported to be associated with marbling, was 95% in cattle and completely fixed in buffalo (Tables 2a and 2b).…”