Novel polymorphisms in ovine <scp><i>prion protein</i></scp> gene

Meydan, Hasan; Özkan, Mustafa; Yıldız, Mehmet Ali; Goldmann, Wilfred

doi:10.1111/age.12019

Cited by 15 publications

(12 citation statements)

References 21 publications

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“…The remaining 18% of the goats carried at least one of the other PrP C variant and this frequency is not dissimilar to European populations (Goldmann 2008). Discounting the very common polymorphism S240P, on an average, one amino acid substitution was found for every 36 genotypes revealing that Turkish goats are at least as genetically varied in this locus as Turkish sheep, for which we genotyped on average 52 animals/substitution (Meydan et al 2013a).…”

Section: Discussionmentioning

confidence: 82%

“…In Turkey, scrapie control breeding programmes have not been established for sheep and goats because there have not been any confirmed scrapie cases. Although, all Turkish native sheep breeds have been PRNP genotyped to evaluate the genetic risk for both classical and atypical scrapie (Meydan et al 2012(Meydan et al , 2013a, this is the first study to genotype PRNP in all Turkish native goat breeds.…”

Section: Discussionmentioning

confidence: 99%

“…With EU decision 2003/100/EC (http://eur-lex.europa.eu/legalcontent/EN/TXT/?uri=CELEX:32003D0100), each member state in the European community has introduced breeding strategies to increase the frequency of the resistance-associated PrP variant, ARR in their sheep populations. PRNP genotyping of all native Turkish sheep breeds led to the identification of a considerable number of polymorphisms and helped to evaluate the genetic risk for both classical and atypical scrapie (Meydan et al 2012(Meydan et al , 2013a.…”

Section: Introductionmentioning

confidence: 99%

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Prion protein gene polymorphisms in Turkish native goat breeds

Meydan

Pehlivan

Özkan

et al. 2017

J Genet

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Susceptibility to 'scrapie' disease in goats is influenced by polymorphisms of the prion protein (PRNP) gene. The aim of this study was to identify PRNP gene polymorphisms in a total of 356 scrapie disease-free goats from 10 Turkish native breeds. Eighteen single-nucleotide polymorphisms were detected in the caprine PRNP open-reading frame. Ten previously described amino acid substitutions (I142M, H143R, N146S, N146D, R151H, R154H, P168Q, R211Q, Q222K and P240S) and two novel dimorphisms (G134E and Q163P) were identified. The strongest association between caprine PRNP and relative resistance to scrapie disease has been reported previously for polymorphisms at codons 146 (S/D) and 222 (K). In the present study, these three PrP variants were relatively rare with 6.3%. This is the first report on PRNP gene variation in Turkish native goat breeds and our knowledge of these polymorphisms will assist goat breeding programmes to reduce the risk of scrapie.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prion protein gene polymorphisms in Turkish native goat breeds

Meydan

Pehlivan

Özkan

et al. 2017

J Genet

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“…Scrapie distribution in goats During the last years, genetic selection has been implemented in Europe and in the United States (Coseddu et al, 2007;Pongolini et al, 2009;Meydan et al, 2013).…”

Section: Scrapie Diagnosismentioning

confidence: 99%

Review: A review on classical and atypical scrapie in caprine: Prion protein gene polymorphisms and their role in the disease

Curcio

Sebastiani

Lorenzo

et al. 2016

Animal

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Scrapie is a naturally occurring transmissible spongiform encephalopathy in sheep and goat. It has been known for~250 years and is characterised by the accumulation of an abnormal isoform of a host-encoded prion protein that leads to progressive neurodegeneration and death. Scrapie is recognised in two forms, classical and atypical scrapie. The susceptibility to both types of scrapie is influenced by polymorphisms of the prion protein gene (PRNP). Sheep susceptibility or resistance to classical scrapie is strongly regulated by the polymorphisms at codons 136, 154 and 171 of the PRNP. The genetic role in atypical scrapie in sheep has been defined by polymorphisms at codons 141, 154 and 171, which are associated with different degrees of risk in the occurrence of the ovine disease. Progress has been achieved in the prevention of scrapie in sheep due to efficient genetic breeding programmes based on eradication and control of the disease. In Europe, the success of these programmes has been verified by applying eradication and genetic selection plans. In general terms, the ovine selection plans aim to eliminate and reduce the susceptible allele and to enrich the resistant allele ARR. During outbreaks all susceptible animals are slaughtered, only ARR/ARR resistant rams and sheep and semi-resistant females are preserved. In the occurrence of scrapie positive goats a complete cull of the flock (stamping out) is performed with great economic loss and severe risk of extinction for the endangered breeds. The ability to select scrapie-resistant animals allows to define new breeding strategies aimed to boost genetic progress while reducing costs during scrapie outbreaks. Allelic variants of PRNP can be protective for caprine scrapie, and the knowledge of their distribution in goats has become very important. Over the past few years, the integration of genetic information on goat populations could be used to make selection decisions, commonly referred to as genetic selection. The objective of this review was to summarise the main findings of polymorphisms of the caprine prion protein (PrP) gene and to discuss the possible application of goat breeding schemes integrating genetic selection, with their relative advantages and limitations.

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“…In Mediterranean countries such as Italy, Greece and Spain ARQ/ARQ is a very frequent genotype detected in animals sick with scrapie (Coseddu et al, 2007;Nonno et al, 2003). However, many authors reported ARQ-associated SNPs related to scrapie-resistance (Acutis et al, 2004;Goldmann et al, 2005;Meydan et al, 2013;González et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Biodiversity and Genetic Polymorphisms Against Scrapie inSopravissanaSheep Breed

Curcio

Lasagna

Sarti

et al. 2015

Italian Journal of Animal Science

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Scrapie is a neurodegenerative disease affecting ovine and it is one of several transmissible spongiform encephalopathies (TSEs). Scrapie is recognized as two forms -classical and atypical. Susceptibility or resistance to classical scrapie is strongly regulated by the polymorphisms at codons 136, 154 and 171 of the PRNP gene. Genetic role in atypical scrapie has been described at codons 141 and 154, these ones are involved in the occurrence of the disease at different risk degrees. The aim of this study was to assess the allelic and genotypic frequencies in Sopravissana breed, an endangered autochthon breed of central Italy; in addition, the presence and the frequency of potential protective ARQ allele variants able to increase scrapie resistance by preserving a higher variability of PRNP were evaluated. Three alleles (ARQ, ARR and AHQ) and seven genotypes were observed based on codons 136, 154 and 171 with different frequencies. Moreover, the entire coding sequence of prion protein (PRNP) of the ARQ/ARQ was sequenced; two non-synonymous L141F (23.8%) and H143R (16.7%), also two synonymous polymorphisms (codons 231 and 237) were found. The results showed that the ARQ/ARQ sheep should be considered as a genetic class, which may potentially include animals with different scrapie susceptibility because of the presence of additional polymorphisms. This may allow the application of alternative strategies for breeding programmes in this endangered breed.

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Novel polymorphisms in ovine prion protein gene

Cited by 15 publications

References 21 publications

Prion protein gene polymorphisms in Turkish native goat breeds

Prion protein gene polymorphisms in Turkish native goat breeds

Review: A review on classical and atypical scrapie in caprine: Prion protein gene polymorphisms and their role in the disease

Biodiversity and Genetic Polymorphisms Against Scrapie inSopravissanaSheep Breed

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