Novel polymorphic microsatellite markers from turmeric, Curcuma longa L. (Zingiberaceae)

Senan, Siju; Kizhakayil, Dhanya; Sheeja, T. E.; Sasikumar, B.; Bhat, Alangar Ishwara; Parthasarathy, V. A.

doi:10.2478/botcro-2013-0002

Cited by 19 publications

(11 citation statements)

References 13 publications

(13 reference statements)

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“…Amplification of multiple bands through SSR marker in the present study could be due to the alloployploidy nature of turmeric (Ravindran et al 2007), where the basic chromosome number is suggested as x = 21, in turn which was originated by dibasic amphidiploidy from x = 9 and x = 12 or by secondary polyploidy (Nair et al 2010;Leong-Skorhickova et al 2007). Multiple bands produced by SSRs in this study is in agreement with other reports of turmeric (Siju et al 2010;Senan et al 2013;Singh et al 2015) and other polyploid species such as potato (Rocha et al 2010) and apple (Liu et al 2014) including allopolyploid species such as wheat (Nicot et al 2004;Sehgal et al 2012).…”

Section: Discussionsupporting

confidence: 91%

“…It possesses several activities such as anti-inflammatory, anti-oxidant, antimicrobial, antiviral, antifungal, insecticidal, nematocidal activity, anti-ageing, lipid lowering effects, antiallergic, immunomodulatory, antidiabetic, and anticancerous properties (Sasikumar 2005;Corcolon et al 2015). Turmeric is predominantly vegetatively propagated and has been reported as triploid (2n = 3x = 63; x = 21) and nonaploid (2n = 9x = 63; x = 7) (Senan et al 2013). Cultivars of turmeric are highly diversified and its taxonomy is quite confusing.…”

Section: Introductionmentioning

confidence: 99%

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EST-SSR marker revealed effective over biochemical and morphological scepticism towards identification of specific turmeric (Curcuma longa L.) cultivars

et al. 2017

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Turmeric (Curcuma longa L., family Zingiberaceae) is one of the most economically important plants for its use in food, medicine, and cosmetic industries. Cultivar identification is a major constraint in turmeric, owing to high degree of morphological similarity that in turn, affects its commercialization. The present study addresses this constraint, using EST-SSR marker based, molecular identification of 8 elite cultivars and 88 accessions in turmeric. Fifty EST-SSR primers were screened against eight cultivars of turmeric (Suroma, Roma, Lakadong, Megha, Alleppey Supreme, Kedaram, Pratibha, and Suvarna); out of which 11 primers showed polymorphic banding pattern. The polymorphic information content (PIC) of these primers ranged from 0.13 to 0.48. However, only three SSR loci (CSSR 14, CSSR 15, and CSSR 18) gave reproducible unique banding pattern clearly distinguishing the cultivars 'Lakadong' and 'Suvarna' from other cultivars tested. These three unique SSR markers also proved to be effective in identification of 'Lakadong' cultivars when analysed with 88 accessions of turmeric collected from different agro-climatic regions. Furthermore, two identified cultivars (Lakadong and Suvarna) could also be precisely differentiated when analysed and based on phylogenetic tree, with other 94 genotypes of turmeric. The novel SSR markers can be used for identification and authentication of two commercially important turmeric cultivars 'Lakadong' and 'Suvarna'.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

EST-SSR marker revealed effective over biochemical and morphological scepticism towards identification of specific turmeric (Curcuma longa L.) cultivars

et al. 2017

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“…During the span of experimentation, only 17 EST-SSR and 35 genomic SSR markers had been reported in turmeric. This limited availability arose the need to expand the then existing repertoire of microsatellite markers for future studies aiming at better estimation of genetic variability for the effective conservation of the genetic resources of turmeric (Senan et al 2013). SSR markers were utilized in the present study due to the fact that they are highly reproducible, abundantly distributed in eukryotic genomes, highly stringent and hypervariable.…”

Section: Resultsmentioning

confidence: 99%

Genetic Variability and Molecular Diversity Analysis in Turmeric (Curcuma longa L.)

Patel¹,

Patel²,

Modha³

et al. 2021

IJARe

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Background: Turmeric is an age old herbaceous plant belonging to the family Zingiberaceae growing in Tropical Africa and India with huge national and international demands. Conventionally, turmeric accessions are characterized using morphological and agronomical traits but less work is done in molecular characterization which is needed for thorough trait identification. Methods: The investigation was carried out during Kharif 2016-17 and comprised of thirty diverse genotypes of turmeric analyzed for genetic variability and molecular diversity which were evaluated in randomized block design with two replications. Fresh tender leaves were used to isolate DNA and PCR was performed with 9 SSR markers. Result: The values of phenotypic coefficient of variation were slightly higher than genotypic coefficient of variation. Highest heritability was observed for days to maturity, genetic advance was observed highest for green rhizome yield. Out of 9 SSR markers, primer pair 5, 6, 7, 8 and 9 were reported to exhibit 100% polymorphism, whereas; in terms of PIC, primer pairs 6 and 9 were found to be highly efficient ones. The similarity coefficient ranged from 0.44 to 1.00 and dendogram categorized the 30 genotypes into two main clusters.

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“…Assessment of genetic diversity using ISSR and RAPD markers were carried out among C.longa and other 14 species of curcuma and placed them into seven groups which are congruent with the morphological characters-based classification (Syamkumar and Sasikumar 2007). Recent progress in molecular markers was the development and characterization of EST-derived and genomic microsatellites which can be used for the diversity analysis of turmeric (Joshi et al 2010;Senan et al 2013).…”

Section: Biotechnological Aspects Of Turmericmentioning

confidence: 98%

Interaction of turmeric (Curcuma longa L.) with beneficial microbes: a review

et al. 2017

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L., commonly known as turmeric, is a rhizomatous herb of the family Zingiberaceae. It is mostly used as a spice, a coloring agent and broadly used in traditional medicine such as Ayurveda, Unani, etc., Turmeric rhizomes interact with a large numbers of rhizosphere-associated microbial species, and some enter the plant tissue and act as endophytes. Both rhizospheric and endophytic species are directly or indirectly involved in growth promotion and disease management in plants and also play an important role in the modulation of morphological growth, secondary metabolite production, curcumin content, antioxidant properties, etc. The present review focuses on the rhizobacterial and endophytic bacterial and fungal populations associated with the turmeric.

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Novel polymorphic microsatellite markers from turmeric, Curcuma longa L. (Zingiberaceae)

Cited by 19 publications

References 13 publications

EST-SSR marker revealed effective over biochemical and morphological scepticism towards identification of specific turmeric (Curcuma longa L.) cultivars

EST-SSR marker revealed effective over biochemical and morphological scepticism towards identification of specific turmeric (Curcuma longa L.) cultivars

Genetic Variability and Molecular Diversity Analysis in Turmeric (Curcuma longa L.)

Interaction of turmeric (Curcuma longa L.) with beneficial microbes: a review

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