Novel poly(<scp>L</scp>‐lactic acid)/hyaluronic acid macroporous hybrid scaffolds: Characterization and assessment of cytotoxicity

Antunes, Joana C.; Oliveira, Joaquím M.; Reis, Rui L.; Soria, José Miguel; Ribelles, J. L. Gomez; Mano, João F.

doi:10.1002/jbm.a.32753

Cited by 48 publications

(31 citation statements)

References 68 publications

(220 reference statements)

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“…Scaffolds, in particular, play an important role in the TE equation as they mimic the function of the natural extracellular matrix, and support cell functions and the formation of new tissues [Oliveira et al, 2009;Antunes et al, 2010]. Scaffolds should meet a number of requirements, such as porosity, bioactivity, and biocompatibility, and have an adequate degradation rate in order to maintain the mechanical strength of the newly formed tissue at the implantation site.…”

mentioning

confidence: 99%

Biofunctional Ionic-Doped Calcium Phosphates: Silk Fibroin Composites for Bone Tissue Engineering Scaffolding

Pina

Canadas

Jiménez³

et al. 2017

Cells Tissues Organs

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The treatment and regeneration of bone defects caused by traumatism or diseases have not been completely addressed by current therapies. Lately, advanced tools and technologies have been successfully developed for bone tissue regeneration. Functional scaffolding materials such as biopolymers and bioresorbable fillers have gained particular attention, owing to their ability to promote cell adhesion, proliferation, and extracellular matrix production, which promote new bone growth. Here, we present novel biofunctional scaffolds for bone regeneration composed of silk fibroin (SF) and β-tricalcium phosphate (β-TCP) and incorporating Sr, Zn, and Mn, which were successfully developed using salt-leaching followed by a freeze-drying technique. The scaffolds presented a suitable pore size, porosity, and high interconnectivity, adequate for promoting cell attachment and proliferation. The degradation behavior and compressive mechanical strengths showed that SF/ionic-doped TCP scaffolds exhibit improved characteristics for bone tissue engineering when compared with SF scaffolds alone. The in vitro bioactivity assays using a simulated body fluid showed the growth of an apatite layer. Furthermore, in vitro assays using human adipose-derived stem cells presented different effects on cell proliferation/differentiation when varying the doping agents in the biofunctional scaffolds. The incorporation of Zn into the scaffolds led to improved proliferation, while the Sr- and Mn-doped scaffolds presented higher osteogenic potential as demonstrated by DNA quantification and alkaline phosphatase activity. The combination of Sr with Zn led to an influence on cell proliferation and osteogenesis when compared with single ions. Our results indicate that biofunctional ionic-doped composite scaffolds are good candidates for further in vivo studies on bone tissue regeneration.

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confidence: 99%

Biofunctional Ionic-Doped Calcium Phosphates: Silk Fibroin Composites for Bone Tissue Engineering Scaffolding

Pina

Canadas

Jiménez³

et al. 2017

Cells Tissues Organs

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“…Problems related to the filling technique, to the in situ crosslinking of the gel, and to the degree of pore coating or filling depending on the gel concentration, had to be addressed there. Other scaffolds with internal gel coating, such as the couples chitosan/fibrin [2], or polylactide/HA [3], have been studied; in each case, the filling and the gelation or crosslinking inside the pores represented specific difficulties to overcome. In the present paper we study constructs consisting of PEA scaffolds with the self-assembling peptide (SAP) RAD16-I hydrogel filling the pores.…”

Section: Introductionmentioning

confidence: 99%

Combining self-assembling peptide gels with three-dimensional elastomer scaffolds

Vallés-Lluch¹,

Arnal-Pastor²,

Martínez‐Ramos³

et al. 2013

Acta Biomaterialia

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ElsevierVallés Lluch, A.; Arnal Pastor, MP.; Martínez Ramos, C.; Vilariño Feltrer, G.; Vikingsson, L.; Castells Sala, C.; Semino, CE.... (2013). Combining self-assembling peptide gels with three-dimensional elastomer scaffolds. Acta Biomaterialia. 9 (12) Abstract: Some of the problems raised by the combination of porous scaffolds and self-assembling peptide (SAP) gels as constructs for tissue engineering applications are for the first time addressed. Scaffolds of poly(ethyl acrylate) and the SAP gel RAD16-I were employed. The in situ gelation of the SAP gel inside the pores of the scaffolds was studied. The scaffold-cum-gel constructs were characterized morphologically, physico-chemically and mechanically. The possibility of incorporating an active molecule (bovine serum albumin, taken here as a model molecule for others) in the gel within the scaffold's pores was assessed, and the kinetics of its release in PBS was followed. Cell seeding and colonization of these constructs was preliminary studied with L929 fibroblasts and checked afterwards with sheep adipose-tissue derived stem cells (ASCs) intended for further preclinical studies. Static (conventional) and dynamically assisted seedings were compared for bare scaffolds and the scaffoldcum-gel constructs. The SAP gel inside the pores of the scaffold significantly improved the uniformity and density of cell colonization of the 3D structure. These constructs could be of use in different advanced tissue engineering applications where, apart from a cell-friendly ECM-like aqueous environment, a larger-scale three-dimensional structure able to keep the cells in a specific place, give mechanical support and/or conduct spatially the tissue growth could be required. COMBINING SELF-ASSEMBLING PEPTIDE GELS WITH 3D ELASTOMER SCAFFOLDSA. AbstractSome of the problems raised by the combination of porous scaffolds and selfassembling peptide (SAP) gels as constructs for tissue engineering applications are for the first time addressed. Scaffolds of poly(ethyl acrylate) and the SAP gel RAD16-I were employed. The in situ gelation of the SAP gel inside the pores of the scaffolds was studied. The scaffold-cum-gel constructs were characterized morphologically, physicochemically and mechanically. The possibility of incorporating an active molecule (bovine serum albumin, taken here as a model molecule for others) in the gel within the scaffold's pores was assessed, and the kinetics of its release in PBS was followed. Cell intended for further preclinical studies. Static (conventional) and dynamically assisted seedings were compared for bare scaffolds and the scaffold-cum-gel constructs. The SAP gel inside the pores of the scaffold significantly improved the uniformity and density of cell colonization of the 3D structure. These constructs could be of use in different advanced tissue engineering applications where, apart from a cell-friendly ECM-like aqueous environment, a larger-scale three-dimensional structure able to keep the cells in a specific place, give mechanical support and/o...

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“…However, there is still a great need to improve other properties, such as the thermal and mechanical properties to increase the scope of applications in the biomedical or environmental plastics fields. One of the most effective methods for enhancing the thermal and mechanical properties of PLA is its copolymerization with other monomers or polymers [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Polylactide-b-Poly (Dimethyl Siloxane) Block Copolymers and Their Blends with Pure Polylactide

Hazer

Baysal²,

Köseoğlu

et al. 2011

J Polym Environ

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The synthesis of polylactide (PLA)-b-poly (dimethyl siloxane) (PDMS) linear block copolymers and their use in blends with pure-PLA are described. PLA-b-PDMS linear block copolymers were obtained by the transesterification reaction in chloroform solution between poly(dimethyl siloxane) bis (2-aminopropyl ether) (molecular weight 2,000 Da) with PLA in the presence of stannous octoate. Molecular weights (Mw) of the block copolymers were varied from 53,800 to 63,600 Da while that of pristine PLA was 73,600 Da. The copolymers obtained were purified by fractional precipitation and then characterized by 1 H NMR, FTIR, GPC, viscometry and DSC techniques. Blends of pure PLA with PLA-b-PDMS block copolymers displayed improved elastic properties (elongation up to 140%) compared to pure PLA (elongation *9%). Thermal, mechanical and morphological characterization of the blends were also conducted.

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Novel poly(L‐lactic acid)/hyaluronic acid macroporous hybrid scaffolds: Characterization and assessment of cytotoxicity

Cited by 48 publications

References 68 publications

Biofunctional Ionic-Doped Calcium Phosphates: Silk Fibroin Composites for Bone Tissue Engineering Scaffolding

Biofunctional Ionic-Doped Calcium Phosphates: Silk Fibroin Composites for Bone Tissue Engineering Scaffolding

Combining self-assembling peptide gels with three-dimensional elastomer scaffolds

Synthesis of Polylactide-b-Poly (Dimethyl Siloxane) Block Copolymers and Their Blends with Pure Polylactide

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