Novel Poly(amidoamine)‐Based Hydrogels as Scaffolds for Tissue Engineering

Abstract

Summary:Poly(amidoamine)s are biocompatible biodegradable polymers, which can be easily functionalized with a number of bioactive and biomimetic compounds. Co‐polymerization of these polymers with 4‐aminobutyl guanidine (agmatine) leads to an RGD mimicking structure. Hydrogels based on this structure showed an enhanced cell adhesion and could be chemically linked to a glass substrate to create a bioadhesive support for cell growth. Preliminary optimization and cell adhesion tests on Madin‐Darby Canine Kidney cells were performed, both on functionalized and non‐functionalized structures, with promising results.

Poly(amidoamine) Hydrogels as Scaffolds for Cell Culturing and Conduits for Peripheral Nerve Regeneration

“…A systematic comparative study of the response of an epithelial cell line has been performed on AGMA1-EDA hydrogels, on nonfunctionalized amphoteric ISA23-EDA hydrogels, and tissue culture plastic substrates [62,65]. As previously pointed out, the AGMA1 repeating units (Figure 1) are very similar to the well-known adhesionmodulating RGD peptide sequence.…”

“…Hydrogel tubes of different dimensions (length 10-30 mm, 3 mm external diameter, and 0. diameter) are obtained ( Figure 11); however, the preferred dimensions for rat implantation are 10 mm length and 1 mm inner diameter [64]. Cross-linked PAAs, obtained by ethylenediamine as cross-linking agent, swell in water giving very soft hydrogels [33,42,62,65]. Swelling tests carried out on the AGMA1-UV hydrogels in doubly distilled water and 0.1 M PBS solution pH 7.4 show a 200% absorption in both cases, 3 times lower than the AGMA1-EDA hydrogels.…”

RGD-mimic polyamidoamine-montmorillonite composites with tunable stiffness as scaffolds for bone tissue-engineering applications

“…The samples were then centrifuged at 13000 rpm for 60 min at 4°C, the supernatants drained and the pellets eluted with 1 n NaOH for 15 min at room temperature (RT). The supernatants (100 µl) were transferred to 96‐well plates and their absorbance was measured at 540 nm (Ozkan et al ., ; Beck et al ., ; Richardson et al ., ; Martello et al ., ; Magnaghi et al ., ; Franchini et al ., ; Ranucci et al ., ; Ferruti et al, , •; Emilitri et al ., ; Jacchetti et al ., ; Dos Reis et al ., ; Mauro et al ., , Iyer et al ., ), using a calibration curve obtained with collagen type I from calf's skin (Sigma, Milan, Italy). For comparison purposes, collagen accumulated by cells grown on TCPS under the same culture conditions was determined.…”

“…Polyamidoamines (PAAs) are well‐known, hydrophilic, biodegradable and biocompatible polymers suited for several biotechnological applications (Ferruti, ). Crosslinked PAAs form hydrogels capable, in several instances, of promoting cell adhesion and proliferation (Ferruti et al ., , ; Emilitri et al ., ; Jacchetti et al ., ; Dos Reis et al , ; Mauro et al ., ). Among these, hydrogels based on AGMA1, a guanidine‐substituted peptidomimetic PAA designed to mimic RGD peptide (Figure ), exhibited particularly good performances both in vitro and in vivo .…”

Poly(amidoamine)s: Past, present, and perspectives

“…These data were later substantially confirmed. 240 In a parallel study, 241 nanometric hydrogel layers based on ISA23 and AGMA1 crosslinked with 1,2-diaminoethane supported on transparent substrates for cell culture were prepared by in situ polymerization carried out on glass substrates purposely modified with c-aminopropyltriethoxysilane, followed by swelling in water, which invariably led to spontaneous delamination of the external bulk of the hydrogel. AGMA1 hydrogel layers exhibited a level of cell adhesion toward epithelial cells (MDCK) comparable to that of commercial plastic substrates.…”