Novel Perspectives on Pituitary and Brain Angiotensinogen

Sernia, Conrad; Zeng, Tang; Kerr, David; Wyse, Bruce D.

doi:10.1006/frne.1997.0150

Cited by 26 publications

(32 citation statements)

References 152 publications

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“…The presence of AGT in rat gonadotropes reported here is in agreement with the previous results of Sernia et al (1992Sernia et al ( ,1997 and Thomas and Sernia (1990). These authors demonstrated AGT secretion by immunocytochemically identified gonadotropes, using the reverse hemolytic plaque assay (Sernia et al 1992).…”

Section: Discussionsupporting

confidence: 93%

“…Deschepper and Ganong (1991) and Ganong et al (1989) reported that AGT is located in a separate population of cells and does not co-localize with any known anterior pituitary hormone. By contrast, Sernia et al (1992Sernia et al ( ,1997 and Thomas and Sernia (1990) detected AGT in gonadotropes and also in a population of perisinusoidal cells. In the rat pituitary, RAS components, either AGT (Ganong et al 1989; Thomas and Sernia 1990;Deschepper and Ga-nong 1991;Sernia et al 1992) or renin (Naruse et al 1981(Naruse et al ,1986, were not detected in lactotropes.…”

mentioning

confidence: 72%

“…This would be consistent with the lysosomal nature of some granulations in rat gonadotropes (Tougard et al 1974), which has also been pos-tulated for lactotropes (Saint-André et al 1989) and for renin-secreting cells in the kidney (Taugner et al 1985;Hackenthal et al 1990;Reudelhuber et al 1993). Many authors have reported that gonadotropes also contain AII (Steele et al 1982;Deschepper et al 1986a;Naruse et al 1986;Ganong et al 1989;Thomas and Sernia 1990;Sernia et al 1997), and it is possible that AGT is processed to AII in these cells. The detection of renin mRNA by in situ hybridization experiments in cells identified as gonadotropes (Deschepper et al 1986b) provides further evidence against an internalization of circulating RAS components in pituitary cells.…”

Section: Discussionmentioning

confidence: 99%

“…Renin has not been detected in rat somatotropes (Naruse et al 1981;Saint-André et al 1989), corticotropes, or thyrotropes (Naruse et al 1981). RAS constituents were not found in folliculostellate cells, identified by their immunoreactivity to the S-100 protein (Ganong et al 1989;Thomas and Sernia 1990;Deschepper and Ganong 1991;Sernia et al 1992Sernia et al ,1997.…”

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confidence: 91%

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Angiotensinogen, Prorenin, and Renin Are Co-localized in the Secretory Granules of All Glandular Cells of the Rat Anterior Pituitary: An Immunoultrastructural Study

Vila-Porcile

Corvol

1998

J Histochem Cytochem.

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SUMMARYIn addition to the circulating renin-angiotensin system (RAS), a local system has been postulated in the anterior pituitary because immunodetection of its components in various mammalian species. However, different cell types appear to be involved in different species, and there is no general consensus on the subcellular localization of prorenin, renin and angiotensinogen. In this ultrastructural study, we investigated and quantified the presence of these components using double or triple immunogold labeling methods, in all the immunologically identified glandular cell types of the rat anterior pituitary. In contrast to previous reports, all these components were identified not only in lactotropes and gonadotropes but also in somatotropes, corticotropes, and thyrotropes. The highest levels were detected in lactotropes and gonadotropes, and renin gave the greatest signal. Angiotensinogen, prorenin, and renin were co-localized in the secretory granules of all rat pituitary glandular cell types. The simultaneous detection of the substrate (angiotensinogen) and both its specific cleavage enzyme and its proenzyme within the same granule suggests intragranular processing of this component. Moreover, the localization of these three constituents in the secretory granules also suggests that, in the rat anterior pituitary, they follow the regulated secretory pathway. (J Histochem Cytochem 46:301-311, 1998)

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Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 91%

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Angiotensinogen, Prorenin, and Renin Are Co-localized in the Secretory Granules of All Glandular Cells of the Rat Anterior Pituitary: An Immunoultrastructural Study

Vila-Porcile

Corvol

1998

J Histochem Cytochem.

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“…This uptake was debated for AGT (Sernia et al 1992(Sernia et al ,1997. Whatever the importance of this phenomenon, the detection of AGT mRNA in GH3 cells by RT-PCR (Sernia 1995) strongly supports the synthesis of AGT by these cells and is consistent with our present hypotheses.…”

Section: Discussionsupporting

confidence: 89%

Secretion of Renin–Angiotensin System (RAS) Components by Normal and Tumoral Lactotropes: A Comparative Study Using Reverse Hemolytic Plaque Assay (RHPA) and Immunoelectron Microscopy

Vila-Porcile

Barret

Corvol

2000

J Histochem Cytochem.

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S U M M A R YImmunodetection of renin-angiotensin system (RAS) components indicates that there is a local RAS in anterior pituitary cells, particularly in lactotropes. We have attempted to determine if RAS molecules are secreted by lactotropes and the secretory pathways and intracellular sites of maturation. We investigated the secretory activity of individual lactotropes, using the reverse hemolytic plaque assay (RHPA), with GH3B6 tumor cells and normal male rat pituitary cells. We also determined the subcellular distributions of RAS components in these cells. Both tumor and normal cells secreted angiotensinogen, prorenin, renin, angiotensin I, angiotensin-converting enzyme, and angiotensin II, although at different levels. The percentage of secretory cells was generally higher in tumor lactotropes than in normal cells. The subcellular distribution of RAS components obtained by immunoperoxidase was very similar in both cell types, although the intensities of immunoreactivity differed. Cleaved and uncleaved components were found in rough endoplasmic reticulum (RER), Golgi saccules, and secretory granules, all compartments of the secretory pathway. The cleaved components in the RER suggest the existence of early maturation, whereas the presence of uncleaved products in the secretory granules of normal lactotropes might indicate late maturation sites.

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Angiotensinogen

Tewksbury

2000

Comprehensive Physiology

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The sections in this article are: Primary Structure Kinetics of the Renin‐Angiotensinogen Reaction Regulation Hormonal Regulation Transcriptional and Translational Regulation In Vivo Manipulation of Angiotensinogen Gene Expression Tissue Renin–Angiotensin Systems Brain Pituitary Kidney Adrenal Heart Vascular Tissue Adipose Tissue Angiotensinogen and the Acute‐Phase Reaction Angiotensinogen and Essential Hypertension Angiotensinogen and Preeclampsia Angiotensinogen and Heart Disease Summary

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Novel Perspectives on Pituitary and Brain Angiotensinogen

Cited by 26 publications

References 152 publications

Angiotensinogen, Prorenin, and Renin Are Co-localized in the Secretory Granules of All Glandular Cells of the Rat Anterior Pituitary: An Immunoultrastructural Study

Angiotensinogen, Prorenin, and Renin Are Co-localized in the Secretory Granules of All Glandular Cells of the Rat Anterior Pituitary: An Immunoultrastructural Study

Secretion of Renin–Angiotensin System (RAS) Components by Normal and Tumoral Lactotropes: A Comparative Study Using Reverse Hemolytic Plaque Assay (RHPA) and Immunoelectron Microscopy

Angiotensinogen

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