Novel perspectives for the application of total internal reflection microscopy

Volpe, Giovanni; Brettschneider, Thomas; Helden, Laurent; Bechinger, Clemens

doi:10.1364/oe.17.023975

Cited by 41 publications

(43 citation statements)

References 36 publications

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“…The trajectories of colloidal particles close to a wall are measured with TIRM [16][17][18] and a scheme of a typical setup is presented in Fig. 5(a).…”

Section: A Total Internal Reflection Microscopymentioning

confidence: 99%

“…Finally, in the Appendix we clarify how to describe correctly such phenomena using both stochastic differential equations and Fokker-Planck equations. Main techniques of measuring forces at the microscopic and nanoscopic scales, classified according to their force resolution and the working conditions-surface and bulkfor which they are best suited: atomic force microscopy (AFM) [12], photonic force microscopy (PFM) [13][14][15], and total internal reflection microscopy (TIRM) [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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Force measurement in the presence of Brownian noise: Equilibrium-distribution method versus drift method

et al. 2011

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The study of microsystems and the development of nanotechnologies require alternative techniques to measure piconewton and femtonewton forces at microscopic and nanoscopic scales. Among the challenges is the need to deal with the ineluctable thermal noise, which, in the typical experimental situation of a spatial diffusion gradient, causes a spurious drift. This leads to a correction term when forces are estimated from drift measurements [G. Volpe, L. Helden, T. Brettschneider, J. Wehr, and C. Bechinger, Phys. Rev. Lett. 104, 170602 (2010)]. Here we provide a systematic study of such an effect by comparing the forces acting on various Brownian particles derived from equilibrium-distribution and drift measurements. We discuss the physical origin of the correction term, its dependence on wall distance and particle radius, and its relation to the convention used to solve the respective stochastic integrals. Such a correction term becomes more significant for smaller particles and is predicted to be on the order of several piconewtons for particles the size of a biomolecule.

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“…The trajectories of colloidal particles close to a wall are measured with TIRM [16][17][18] and a scheme of a typical setup is presented in Fig. 5(a).…”

Section: A Total Internal Reflection Microscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Force measurement in the presence of Brownian noise: Equilibrium-distribution method versus drift method

et al. 2011

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“…Therefore, a key question is how particle dynamics are affected through HI by the existence of nonpenetrable walls. Different techniques have been used to study near wall dynamics, including evanescent wave dynamic light scattering (EWDLS), [10][11][12][13][14][15][16] confocal optical microscopy, 17 total internal reflection microscopy, 18,19 resonance enhanced scattering, 20,21 and laser tweezers. 22 The hydrodynamic interactions between a planar solid surface and a nearby Brownian sphere have been calculated at the level of a single particle as early as the beginning of the 20th century 23 and latter in the 1960s.…”

Section: Introductionmentioning

confidence: 99%

Anisotropic diffusion of concentrated hard-sphere colloids near a hard wall studied by evanescent wave dynamic light scattering

Michailidou

Swan

Brady

et al. 2013

The Journal of Chemical Physics

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Evanescent wave dynamic light scattering and Stokesian dynamics simulations were employed to study the dynamics of hard-sphere colloidal particles near a hard wall in concentrated suspensions. The evanescent wave averaged short-time diffusion coefficients were determined from experimental correlation functions over a range of scattering wave vectors and penetration depths. Stokesian dynamics simulations performed for similar conditions allow a direct comparison of both the shorttime self-and collective diffusivity. As seen earlier [V. N. Michailidou, G. Petekidis, J. W. Swan, and J. F. Brady, Phys. Rev. Lett. 102, 068302 (2009)] while the near wall dynamics in the dilute regime slow down compared to the free bulk diffusion, the reduction is negligible at higher volume fractions due to an interplay between the particle-wall and particle-particle hydrodynamic interactions. Here, we provide a comprehensive comparison between experiments and simulations and discuss the interplay of particle-wall and particle-particle hydrodynamics in the self-and cooperative dynamics determined at different scattering wave vectors and penetration depths. © 2013 AIP Publishing LLC.

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“…We developed two calibration methods that rely on the actual system itself and do not require adding further optics to the setup. One is based on the 3D distribution of tethered beads (Volpe, Brettschneider et al 2009). The distribution is measured with TIR illumination and the persistence length is calculated from the planar x and y distributions.…”

Section: Methodsmentioning

confidence: 99%