Novel p22 and p30 dual-proteins combination based indirect ELISA for detecting antibodies against African swine fever virus

Li, Jianda; Jiao, Jian; Liu, Na; Ren, Sufang; Zeng, Hao; Peng, Jun; Zhang, Yuyu; Guo, Lihui; Liu, Fei; Lv, Tingting; Chen, Zhi; Sun, Wei; Hrabchenko, Nataliia; Yu, Junsheng; Wu, Jiasheng

doi:10.3389/fvets.2023.1093440

Cited by 7 publications

(7 citation statements)

References 34 publications

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“…As presented in Table 3, in response to the emergence of low virulent ASFV strains with CD2v mutants, Lv et al (78) developed a dual ELISA approach utilizing CD2v and p30 protein to differentiate wild-type strains from CD2v-deleted strains with low virulence. Furthermore, indirect ELISA methods based on non-structural proteins of ASFV, including pB602L (79), pp62 (80), pK205R (81), I329L (82), p11.5 (83), CD2v (84), p22 and p30 (85,86), have demonstrated favorable detection capabilities, with an analytical sensitivity ranging from 1:1280 to 1:12800 (Table 3). In comparison to indirect ELISA, the main advantage of blocking (competitive) ELISA is its high sensitivity to compositional differences in complex antigen mixtures, even when the specific detecting antibody is present in relatively small amounts (125).…”

Section: Asfv Associated Antibodies Detection Elisamentioning

confidence: 99%

Current detection methods of African swine fever virus

Hu,

Tian,

Lai

et al. 2023

Front. Vet. Sci.

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African swine fever (ASF), caused by the African swine fever virus (ASFV), is a highly contagious and notifiable animal disease in domestic pigs and wild boars, as designated by the World Organization for Animal Health (WOAH). The effective diagnosis of ASF holds great importance in promptly controlling its spread due to its increasing prevalence and the continuous emergence of variant strains. This paper offers a comprehensive review of the most common and up-to-date methods established for various genes/proteins associated with ASFV. The discussed methods primarily focus on the detection of viral genomes or particles, as well as the detection of ASFV associated antibodies. It is anticipated that this paper will serve as a reference for choosing appropriate diagnostic methods in diverse application scenarios, while also provide direction for the development of innovative technologies in the future.

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Section: Asfv Associated Antibodies Detection Elisamentioning

confidence: 99%

Current detection methods of African swine fever virus

Hu,

Tian,

Lai

et al. 2023

Front. Vet. Sci.

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“…Indirect ELISA (iELISA) has become the most suitable and common method for ASFV detection in large amounts of serum (Li et al, 2023). Recently, an indirect ELISA method was developed to detect ASFV antibodies using recombinant pp62 protein.…”

Section: Indirect Elisamentioning

confidence: 99%

“…This assay has excellent specificity, sensitivity, and repeatability (Zhong et al, 2022). Several indirect ELISA methods have been developed using other recombinant proteins such as p17 (Li et al, 2023) and CD2v (Jiang et al, 2022). Multiantigenic protein combinations have also been utilized to specifically detect ASFV antibodies, such as the p22-p30 (Li et al, 2023) and the p30-p54-p72 combination .…”

Section: Indirect Elisamentioning

confidence: 99%

Current progress in diagnostics, therapeutics, and vaccines for African swine fever virus

Orosco

2023

VIS

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African Swine Fever Virus (ASFV) is a highly contagious viral disease that affects domestic and wild pigs. Due to its high mortality rate and rapid spread, it poses a significant threat to the global swine industry. There is currently no effective treatment for ASFV, and control strategies rely on early detection and culling of infected animals. Therefore, developing efficient diagnostics, therapeutics, and vaccines for ASFV is crucial for preventing its spread and minimizing the economic losses associated with outbreaks. In recent years, significant progress has been made in developing diagnostic tools for ASFV, including serological, molecular, and cell-based assays. Therapeutic interventions for ASFV are limited, with no approved treatments currently available. However, recent studies have explored the potential of antiviral drugs and immunomodulators as potential therapies for ASFV. Meanwhile, vaccines have been developed using different platforms, including live attenuated viruses, subunit vaccines, and viral vectors. Some of these vaccines have shown promising results in inducing both humoral and cell-mediated immune responses, but challenges remain in terms of vaccine efficacy. Therefore, significant progress has been made in developing diagnostics, therapeutics, and vaccines for ASFV, but much work remains to be done. Further research is needed to improve the efficacy and safety of current interventions and to develop new tools for controlling ASFV globally.

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“…To date, several conventional laboratory diagnostic techniques have been used for the detection of ASF, including polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), fluorescent quantitative PCR, colloidal gold rapid test strips, and ELISA [ 16 , 17 ]. In particular, ELISA is the most commonly used specific antibody assay and is a designated experiment specified by WOAH (World Organization for Animal Health) for international trade to detect specific antibodies to ASFV [ 18 ]. ELISAs include direct, indirect, competition, and sandwich ELISAs, which have the advantages of being efficient, rapid, and easy to perform.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Dual-Antigen Indirect ELISA Based on p30 and pB602L to Detect Antibodies against African Swine Fever Virus

Zhou,

Song,

Wang

et al. 2023

Viruses

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African swine fever (ASF) is an acute, virulent, and highly fatal infectious disease caused by the African swine fever virus (ASFV). There is no effective vaccine or diagnostic method to prevent and control this disease currently, which highlights the significance of ASF early detection. In this study, we chose an early antigen and a late-expressed antigen to co-detect the target antibody, which not only helps in early detection but also improves accuracy and sensitivity. CP204L and B602L were successfully expressed as soluble proteins in an Escherichia coli vector system. By optimizing various conditions, a dual-antigen indirect ELISA for ASFV antibodies was established. The assay was non-cross-reactive with antibodies against the porcine reproductive and respiratory syndrome virus, classical swine fever virus, porcine circovirus type 2, and pseudorabies virus. The maximum serum dilution for detection of ASFV-positive sera was 1:1600. The intra-batch reproducibility coefficient of variation was <5% and the inter-batch reproducibility coefficient of variation was <10%. Compared with commercial kits, the dual-antigen indirect ELISA had good detection performance. In conclusion, we established a detection method with low cost, streamlined production process, and fewer instruments. It provides a new method for the serological diagnosis of ASF.

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Novel p22 and p30 dual-proteins combination based indirect ELISA for detecting antibodies against African swine fever virus

Cited by 7 publications

References 34 publications

Current detection methods of African swine fever virus

Current detection methods of African swine fever virus

Current progress in diagnostics, therapeutics, and vaccines for African swine fever virus

Establishment of a Dual-Antigen Indirect ELISA Based on p30 and pB602L to Detect Antibodies against African Swine Fever Virus

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