Novel observations of Thiobacterium, a sulfur-storing Gammaproteobacterium producing gelatinous mats

Grünke, Stefanie; Lichtschlag, Anna; Beer, Dirk de; Kuypers, Marcel M. M.; Lösekann-Behrens, Tina; Ramette, Alban; Boetius, Antje

doi:10.1038/ismej.2010.23

Cited by 12 publications

(10 citation statements)

References 52 publications

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“…S2B). Bacteria closely related to the alphaproteobacterial, epsilonproteobacterial and Bacteroidetes sequences included (i) bacteria associated with other Osedax species (Goffredi et al ., 2005; 2007); (ii) free‐living bacteria found at whale‐falls (Goffredi et al ., 2005; Tringe et al ., 2005; Goffredi and Orphan, 2010); (iii) free‐living bacteria from the Tjärnö aquarium in Sweden where the whale bones were kept (Grünke et al ., 2010); and (iv) symbionts of other marine invertebrates (Fig. S2B).…”

Section: Resultsmentioning

confidence: 99%

High symbiont diversity in the bone‐eating worm Osedax mucofloris from shallow whale‐falls in the North Atlantic

Verna

Ramette

Wiklund

et al. 2010

Environmental Microbiology

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SummaryOsedax worms are whale-fall specialists that infiltrate whale bones with their root tissues. These are filled with endosymbiotic bacteria hypothesized to provide their hosts with nutrition by extracting organic compounds from the whale bones. We investigated the diversity and distribution of symbiotic bacteria in Osedax mucofloris from shallow-water whale-falls in the North Atlantic using comparative 16S rRNA sequence analysis and fluorescence in situ hybridization (FISH). We observed a higher diversity of endosymbionts than previously described from other Osedax species. Endosymbiont sequences fell into eight phylogenetically distinct clusters (with 91.4-98.9% similarity between clusters), and considerable microdiversity within clusters (99.5-99.7% similarity) was observed. Statistical tests revealed a highly significant effect of the host individual on endosymbiont diversity and distribution, with 68% of the variability between clusters and 40% of the variability within clusters explained by this effect. FISH analyses showed that most host individuals were dominated by endosymbionts from a single cluster, with endosymbionts from less abundant clusters generally confined to peripheral root tissues. The observed diversity and distribution patterns indicate that the endosymbionts are transmitted horizontally from the environment with repeated infection events occurring as the host root tissues grow into the whale bones.

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Section: Resultsmentioning

confidence: 99%

High symbiont diversity in the bone‐eating worm Osedax mucofloris from shallow whale‐falls in the North Atlantic

Verna

Ramette

Wiklund

et al. 2010

Environmental Microbiology

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“…Microsensors for sulfide, pH and oxygen (Revsbech and Ward, 1983;Jeroschewski et al, 1996;de Beer et al, 1997), as well as a macro temperature sensor (Pt100; UST Umweltsensortechnik GmbH, Geschwenda, Germany), were mounted on the profiler. In addition, ex situ microprofiles were measured in retrieved push cores with a laboratory setup as previously described (Grünke et al, 2010). Microsensor calibration, measurements, profile analyses and calculation of fluxes were performed as previously described by and Lichtschlag et al (2010).…”

Section: Microsensor Measurementsmentioning

confidence: 99%

“…Published by Copernicus Publications on behalf of the European Geosciences Union. source: https://doi.org/10.7892/boris.91383 | downloaded: 11.9.2020 2948 S. Grünke et al: Thiotrophic mats at polar cold seeps (Gammaproteobacteria; Grünke et al, 2010) and free-living Arcobacter spp. (Epsilonproteobacteria; Omoregie et al, 2008;Grünke et al, 2011) also form dense mats on the seafloor above gassy sediments.…”

Section: Introductionmentioning

confidence: 99%

Mats of psychrophilic thiotrophic bacteria associated with cold seeps of the Barents Sea

Grünke¹,

Lichtschlag²,

Beer³

et al. 2012

Preprint

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Abstract. This study investigated the bacterial diversity associated with microbial mats of polar deep-sea cold seeps. The mats are associated with high upward fluxes of sulfide produced by anaerobic oxidation of methane, and grow at temperatures close to the freezing point of seawater. They ranged from small patches of 0.2–5 m in diameter (gray mats) to extensive fields covering up to 850 m2 of seafloor (white mats) and were formed by diverse sulfide-oxidizing bacteria differing in color and size. Overall, both the dominant mat-forming thiotrophs as well as the associated bacterial communities inhabiting the mats differed in composition for each mat type as determined by microscopy, 16S rRNA gene sequencing and Automated Ribosomal Intergenic Spacer Analysis. While the smaller gray mats were associated with a highly diverse composition of sulfide oxidizers, the large white mats were composed of only 1–2 types of gliding Beggiatoa filaments. Molecular analyses showed that most of the dominant mat-forming sulfide oxidizers were phylogenetically different from, but still closely related to thiotrophs known from warmer ocean realms. The psychrophilic nature of the polar mat-forming thiotrophs was tested by visual observation of active mats at in situ temperature compared to their warming to >4 °C. The temperature range of mat habitats and the variation of sulfide and oxygen fluxes appear to be the main factors supporting the diversity of mat-forming thiotrophs in cold seeps at continental margins.

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“…(Gammaproteobacteria; Grünke et al, 2010) and free-living Arcobacter spp. (Epsilonproteobacteria; Omoregie et al, 2008;Grünke et al, 2011) also form dense mats on the seafloor above gassy sediments.…”

Section: Introductionmentioning

confidence: 99%

Mats of psychrophilic thiotrophic bacteria associated with cold seeps of the Barents Sea

et al. 2012

View full text Add to dashboard Cite

This study investigated the bacterial diversity associated with microbial mats of polar deep-sea cold seeps. The mats were associated with high upward fluxes of sulfide produced by anaerobic oxidation of methane, and grew at temperatures close to the freezing point of seawater. They ranged from small patches of 0.2–5 m in diameter (gray mats) to extensive fields covering up to 850 m2 of seafloor (white mats) and were formed by diverse sulfide-oxidizing bacteria differing in color and size. Overall, both the dominant mat-forming thiotrophs as well as the associated bacterial communities inhabiting the mats differed in composition for each mat type as determined by microscopy, 16S rRNA gene sequencing and automated ribosomal intergenic spacer analysis. While the smaller gray mats were associated with a highly diverse composition of sulfide oxidizers, the larger white mats were composed of only 1–2 types of gliding Beggiatoa filaments. Molecular analyses showed that most of the dominant mat-forming sulfide oxidizers were phylogenetically different from, but still closely related to, thiotrophs known from warmer ocean realms. The psychrophilic nature of the polar mat-forming thiotrophs was tested by visual observation of active mats at in situ temperature compared to their warming to >4 °C. The temperature range of mat habitats and the variation of sulfide and oxygen fluxes appear to be the main factors supporting the diversity of mat-forming thiotrophs in cold seeps at continental margins

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Novel observations of Thiobacterium, a sulfur-storing Gammaproteobacterium producing gelatinous mats

Cited by 12 publications

References 52 publications

High symbiont diversity in the bone‐eating worm Osedax mucofloris from shallow whale‐falls in the North Atlantic

High symbiont diversity in the bone‐eating worm Osedax mucofloris from shallow whale‐falls in the North Atlantic

Mats of psychrophilic thiotrophic bacteria associated with cold seeps of the Barents Sea

Mats of psychrophilic thiotrophic bacteria associated with cold seeps of the Barents Sea

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