Novel naturally derived whey protein isolate and aragonite biocomposite hydrogels have potential for bone regeneration

Gupta, Dhanak; Kocot, Magdalena; Tryba, Anna Maria; Serafim, Andrada; Stancu, Izabela‐Cristina; Jaegermann, Z.; Pamuła, Elżbieta; Reilly, Gwendolen C.; Douglas, Timothy E.L.

doi:10.1016/j.matdes.2019.108408

Cited by 29 publications

(29 citation statements)

References 29 publications

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“…The band at 1520 cm −1 corresponds to the secondary amide group of proteins (−CO−NH) [ 40 ]. The range 1400–1200 cm −1 was attributed to the amide III [ 41 ]. The observed characteristic band at around 1040 cm −1 was assigned to the stretching of C−O vibrations [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, after a 7-day culture on WPI hydrogel modified with α-TCP, osteoblast-like cells showed higher metabolic activity [ 47 ]. The experiments of Gupta et al showed that argonite-modified WPI hydrogels with different pore diameters promoted osteoblast proliferation in a resazurin reduction assay over a 3-week culture period [ 41 ]. Although the properties of HAp may appear similar because they share the same chemical formula, the way they are synthesized and processed may affect their biological properties.…”

Section: Discussionmentioning

confidence: 99%

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Composites Based on Hydroxyapatite and Whey Protein Isolate for Applications in Bone Regeneration

et al. 2021

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Hydroxyapatite (HAp) is a bioactive ceramic with great potential for the regeneration of the skeletal system. However, its mechanical properties, especially its brittleness, limit its application. Therefore, in order to increase its ability to transmit stresses, it can be combined with a polymer phase, which increases its strength without eliminating the important aspect of bioactivity. The presented work focuses on obtaining organic–inorganic hydrogel materials based on whey protein isolate (WPI) reinforced with nano-HAp powder. The proportion of the ceramic phase was in the range of 0–15%. Firstly, a physicochemical analysis of the materials was performed using XRD, FT-IR and SEM. The hydrogel composites were subjected to swelling capacity measurements, potentiometric and conductivity analysis, and in vitro tests in four liquids: distilled water, Ringer’s fluid, artificial saliva, and simulated body fluid (SBF). The incubation results demonstrated the successful formation of new layers of apatite as a result of the interaction with the fluids. Additionally, the influence of the materials on the metabolic activity according to ISO 10993-5:2009 was evaluated by identifying direct contact cytotoxicity towards L-929 mouse fibroblasts, which served as a reference. Moreover, the stimulation of monocytes by hydrogels via the induction of nuclear factor (NF)-κB was investigated. The WPI/HAp composite hydrogels presented in this study therefore show great potential for use as novel bone substitutes.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Composites Based on Hydroxyapatite and Whey Protein Isolate for Applications in Bone Regeneration

et al. 2021

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“…Whey protein isolate (WPI) is a dairy industry by-product which contains > 95% protein, of which 75% is β-lactoglobulin [1]. Previous studies showed that WPI enhances cell proliferation and osteogenic differentiation and displays antibacterial properties [1][2][3]. Upon heating for several hours under acidic conditions (<pH 3), β-lactoglobulin degrades into smaller peptides, which undergo self-assembly to form amyloid fibrils several micrometers long and a few nm thick [4].…”

Section: Introductionmentioning

confidence: 99%

Dairy-Inspired Coatings for Bone Implants from Whey Protein Isolate-Derived Self-Assembled Fibrils

Rabe

Hempel

Martocq

et al. 2020

IJMS

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To improve the integration of a biomaterial with surrounding tissue, its surface properties may be modified by adsorption of biomacromolecules, e.g., fibrils. Whey protein isolate (WPI), a dairy industry by-product, supports osteoblastic cell growth. WPI’s main component, β-lactoglobulin, forms fibrils in acidic solutions. In this study, aiming to develop coatings for biomaterials for bone contact, substrates were coated with WPI fibrils obtained at pH 2 or 3.5. Importantly, WPI fibrils coatings withstood autoclave sterilization and appeared to promote spreading and differentiation of human bone marrow stromal cells (hBMSC). In the future, WPI fibrils coatings could facilitate immobilization of biomolecules with growth stimulating or antimicrobial properties.

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“…These biomaterials have been explored for tissue engineering applications due to their chemical and biological properties, such as the ability to retain water, easy transport/entrapment of nutrients or cells, controlled biodegradability, mechanical properties and biocompatibility [5]. However, the field of action of whey is much wider and applied to other industries as an eco-friendly alternative to conventional chemicals.…”

Section: Natural By-product and Waste Compositions And Properties 21mentioning

confidence: 99%

Innovation of Textiles through Natural By-Products and Wastes

Reis¹,

Coelho²,

María³

et al. 2020

Waste in Textile and Leather Sectors

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Nowadays, the competitiveness of the textile industry and the consumers' interest have been increasing the demand for innovative and functional textiles. Allied to this, sustainable developments are playing an increasingly important role in the textile industry. Such concerns led to a new development strategy based on the valorization of bio-based wastes and by-products of different industries, inserting this in the circular economy paradigm. These bio-based wastes and by-products come from several industries, as the agri-food industry. These resources present an enormous potential for valorization in the textile finish due to their intrinsic properties (antimicrobial, prebiotic, antioxidant activity, among others). This chapter will review the latest innovation and textile product development through different by-products and wastes, their main properties and characteristics and the advantages that they offer to the textile industry.

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Novel naturally derived whey protein isolate and aragonite biocomposite hydrogels have potential for bone regeneration

Cited by 29 publications

References 29 publications

Composites Based on Hydroxyapatite and Whey Protein Isolate for Applications in Bone Regeneration

Composites Based on Hydroxyapatite and Whey Protein Isolate for Applications in Bone Regeneration

Dairy-Inspired Coatings for Bone Implants from Whey Protein Isolate-Derived Self-Assembled Fibrils

Innovation of Textiles through Natural By-Products and Wastes

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