Novel Natural Bovine Bone Graft with Integrated Atelo-Collagen Type I: Atelocollagen Bovine Bone Mineral (ABBM) Characterization In-vivo

Raouf, Mustafa Abd El; Zhang, Yufeng; Caballé‐Serrano, Jordi; Miron, Richard J.

doi:10.21767/2471-3082.100029

Cited by 2 publications

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“…Водночас ні один із методів остеосинтезу чи використання різноманітних конструкцій не забезпечують відновлення остеокондуктивності та не володіють остеоінтегріційними властивостями для відновлення репаративного потенціалу кісткової тканини у зоні дефекту. В зв'язку з цим застосування губчастої аутокістки, яка за своїми властивостями володіє остеогенними, остеоіндуктивними і остеокондуктивними характеристиками, вважається золотим стандартом кісткової аутопластики (Abd El Raouf et al, 2017;Alireza et al, 2017), але водночас має низку вагомих недоліків. Це потреба в додатковому оперативному доступі з подальшим нанесенням травми для відбору аутоімпланта, обмежена кількість такого пластичного матеріалу, можливі післяопераційні ускладнення в ділянці забору аутокістки (Finkemeier, 2002;Dubrov et al, 2019).…”

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Dynamics of cytokines in osteosis splinter fractures replacement in dogs with hydroxyapatite ceramics silicon-doped

et al. 2021

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Among the entire public of fractures of long bones, more complications of reparative osteogenesis occur fragmental fractures. Their significant defects, which arise as a result of the removal of non-viable fragments, cause disturbances in local hemodynamics and microcirculation and loss of osteoconduction in the area of injury, lead to a loss of the reparative potential of bone tissue in the defect zone, and the methods of osteosynthesis do not provide restoration of osteoconduction and do not have osteointegration properties. For osteosis, a number of composite materials have been proposed, among which ceramics based on calcium phosphates are considered the most promising, which is confirmed by previous clinical, radiological and histomorphological studies. The aim of the study is to determine the dynamics of the blood levels of TGF-β and interleukins IL-1β and IL-10 for osteosis replacement of splinter bone fractures in dogs with calcium phosphate ceramics doped with silicon. Material and research methods: a control (n = 7) and research group (n = 7) dogs with accidental splinter fractures of long bones were formed. In both groups, extracortical osteosynthesis was performed with a support plate from an unalloyed titanium alloy. In the control group, bone defects were left to heal under a blood clot, and in the experimental group they were replaced with ceramics (GTlKg-3). Blood samples were taken after trauma no later than on the 48th day, and on the third, twelfth, twenty-first, forty-second and 60th days after surgical treatment. The enzyme immunoassay was used to determine the serum levels of interleukins IL-1β and IL-10 and transforming growth factor (TGF-β) тhat for a bureta reaction is a bit of a home-made bottle. The study results showed an anti-inflammatory cytokine profile with physiological norms in dogs. In case of bone injury, it becomes pro-inflammatory in nature, triggers the process of osteoresorption in the fracture area. At the same time, during reparative osteogenesis after extracortical osteosynthesis for splinter fractures, proinflammatory cytokinemia acquires a permanent character with a peak on the forty-second day, and this implies inhibition of metaplasia of cartilaginous regenerate into bone, that is, it slows down the consolidation of the fracture. However, in the case of osteosis with ceramics, the level of pro-inflammatory cytokinemia is significantly lower and continues during the first 12 days with a peak on the third day, during the inflammatory-resorptive stage. However, the key in understanding the effectiveness of osteosis was the dynamics of TGF-β, which showed its biphasic hyperreactivity in control animals, while in experimental animals, its balanced production was noted. Conclusion. Osteosis replacement of bone defects in long tubular bones in dogs with hydroxyapatite with β-tricalcium phosphate ceramics doped with silicon is accompanied by moderate cytokinemia in accordance with the course of the inflammatory-resorptive stage and the phase of remodeling with a peak of induction of transforming growth factor during the period of active osteogenesis forty-second course of reparative osteogenesis.

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Dynamics of cytokines in osteosis splinter fractures replacement in dogs with hydroxyapatite ceramics silicon-doped

et al. 2021

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Cytokine profiles in healthy animals and in cows with purulent-necrotic limb processes

Melnikov

Rublenko

Ilnitskiy

2022

Nauk. vìsn. vet. med.

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One of the typical and mandatory reactions of the body to trauma or infectious agents for damage to any tissues and organs is the acute phase reaction, which is an induced increase in the synthesis followed by an increase in the blood and tissues of a number of proteins with immunological, bactericidal, antioxidant and inhibitory functions. The purpose of the study is to determine the levels of cytokines in clinically healthy cows, pigs and dogs, as well as in cows with purulent-necrotic processes of the limbs. The levels of pro-inflammatory cytokines and anti-inflammatory IL-10 were determined in blood serum of cows, pigs and dogs. Cows with purulent-necrotic lesions of the limbs (n=26) were divided into: 1st group (n=8) – acute form of purulent-necrotic lesions of the distal limbs; 2nd group (n=8) – generalized lesions; group 3 (n=10) – recurrent foci in the area of the fingers. The content of TNF-α, IL-1ß and IL-10 interleukins in blood serum was determined by the immunoenzymatic method according to the standard protocol. It was established that the blood levels of pro-inflammatory TNF-α and IL-1ß in clinically healthy cows are significantly lower than anti-inflammatory IL-10, for the ratio of IL-10:TNF-α – 3.3:1, and IL- 10: IL- 1ß - 9.5:1, therefore, for cattle under physiological norms, an inherent anti-inflammatory cytokine profile. In clinically healthy pigs, the anti-inflammatory cytokine profile turned out to be the most pronounced, as the cytokine indices in them were significantly higher: IL-10:TNF-α – 19.4:1; IL-10:IL-1β – 13.9:1. In the blood of clinically healthy dogs, the cytokine indices between IL-10:IL-1ß are extremely low - 1.5:1, TNF-α : IL-1ß - 0.2:1, even lower, and between IL-10:TNF-α - 8.8:1, and therefore the anti-inflammatory cytokine profile is much lower according to the totality of cytokine indices. In cows with an acute form of necrobacteriosis, compared with clinically healthy animals, the level of TNF-α in the blood is 5.6 times higher (Р<0.001), and IL-1ß is 3.4 times higher (Р<0.001), due to their increase index by 1.7 times, up to 4.9:1. Under such conditions, the level of IL-10 increases only 1.8 times (р<0.05). Animals with the generalized form are characterized by a critical increase in the blood level of TNF-α by 16.8 times and IL-1ß by 17.8 times (p <0.001), while the level of IL-10 remains unchanged compared to the acute form. Cytokine index IL-10:TNF-α acquires a critical value - 0.4:1, and IL-10 to IL-1ß - 1:1. In animals with recurrent lesions, low levels of IL-1ß, especially IL-10, are characteristic. However, the concentration of TNF-α remains quite high and exceeds the normal value by 12.6 times (p<0.001). Therefore, various clinical forms of necrobacterial lesions of the limbs in cows have a compensatory or non-compensatory nature of cytokinemia, with an imbalance of the functionality of acute-phase proteins due to the insufficient capacity of the inhibitory potential of sick cows. Key words: cows, pigs, dogs, cytokines, serum, clinically healthy animals, inflammation.

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Novel Natural Bovine Bone Graft with Integrated Atelo-Collagen Type I: Atelocollagen Bovine Bone Mineral (ABBM) Characterization In-vivo

Cited by 2 publications

References 30 publications

Dynamics of cytokines in osteosis splinter fractures replacement in dogs with hydroxyapatite ceramics silicon-doped

Dynamics of cytokines in osteosis splinter fractures replacement in dogs with hydroxyapatite ceramics silicon-doped

Cytokine profiles in healthy animals and in cows with purulent-necrotic limb processes

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