Novel nanoscale bacteriophage-based single-domain antibodies for the therapy of systemic infection caused by Candida albicans

Dong, Shuai; Shi, Hongxi; Cao, Donghui; Wang, Yicun; Zhang, Xintong; Li, Yan; Gao, Xiang; Wang, Li

doi:10.1038/srep32256

Cited by 10 publications

(2 citation statements)

References 43 publications

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“…The resultant phage coat protein displayed peptide library is known as a phage library, which is usually used in the affinity selection (called biopanning) to identify the functional regions of proteins associated with targets, including small molecules, cells, tissues, and even patients. (Dong et al, , ; Krag et al, ; Staquicini et al, ) Phage biopanning technique is unique in that no understanding about the interaction is needed before the selection process. In the past 30 years, tremendous advancement in the phage display technique and the enrichment of its applications described further reviews (Arap, ; Barbass, Burton, Scott, & Silverman, ; Bratkovič, ; Clackson & Lowman, ; Kay, Winter, & McCafferty, ; Kehoe & Kay, ; Marvin, ; Matsumura, Doi, & Yanagawa, ; Sergeeva, Kolonin, Molldrem, Pasqualini, & Arap, ; Sidhu, ; Smith & Petrenko, ; Szardenings, ).…”

Section: Introductionmentioning

confidence: 99%

Phage nanofibers in nanomedicine: Biopanning for early diagnosis, targeted therapy, and proteomics analysis

Cao

et al. 2020

WIREs Nanomed Nanobiotechnol

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Display of a peptide or protein of interest on the filamentous phage (also known as bacteriophage), a biological nanofiber, has opened a new route for disease diagnosis and therapy as well as proteomics. Earlier phage display was widely used in protein–protein or antigen–antibody studies. In recent years, its application in nanomedicine is becoming increasingly popular and encouraging. We aim to review the current status in this research direction. For better understanding, we start with a brief introduction of basic biology and structure of the filamentous phage. We present the principle of phage display and library construction method on the basis of the filamentous phage. We summarize the use of the phage displayed peptide library for selecting peptides with high affinity against cells or tissues. We then review the recent applications of the selected cell or tissue targeting peptides in developing new targeting probes and therapeutics to advance the early diagnosis and targeted therapy of different diseases in nanomedicine. We also discuss the integration of antibody phage display and modern proteomics in discovering new biomarkers or target proteins for disease diagnosis and therapy. Finally, we propose an outlook for further advancing the potential impact of phage display on future nanomedicine. This article is categorized under: Biology‐Inspired Nanomaterials > Protein and Virus‐Based Structures

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Section: Introductionmentioning

confidence: 99%

Phage nanofibers in nanomedicine: Biopanning for early diagnosis, targeted therapy, and proteomics analysis

Cao

et al. 2020

WIREs Nanomed Nanobiotechnol

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“…20,21 In our previous studies, phage-displaying anti-rSap2 scFvs were identified, which targeted both C. albicans cells and treated systemic infection caused by C. albicans. 22 Therefore, phage-display antibody libraries can be readily screened for specific binders to the antigen proteins presented on cells by biopanning. 23 The cell wall of C. albicans is a crucial factor in maintaining structural integrity, and is comprised of two main layers.…”

Section: Introductionmentioning

confidence: 99%

Difunctional bacteriophage conjugated with photosensitizers for <em>Candida albicans</em>-targeting photodynamic inactivation

Dong¹,

Shi²,

Zhang³

et al. 2018

IJN

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BackgroundCandida albicans is the most prevalent fungal pathogen of the human microbiota, causing infections ranging from superficial infections of the skin to life-threatening systemic infections. Due to the increasing occurrence of antibiotic-resistant C. albicans strains, new approaches to control this pathogen are needed. Photodynamic inactivation is an emerging alternative to treat infections based on the interactions between visible light and photosensitisers, in which pheophorbide a (PPA) is a chlorophyll-based photosensitizer that could induce cell death after light irradiation. Due to PPA’s phototoxicity and low efficiency, the main challenge is to implement photosensitizer cell targeting and attacking.MethodsIn this study, PPA was conjugated with JM-phage by EDC/NHS crosslinking. UV-Vis spectra was used to determine the optimum conjugation percentages of PPA and JM-phage complex for photodynamic inactivation. After photodynamic inactivation, the efficacy of PPA-JM-phage was assessed by performing in vitro experiments, such as MTS assay, scanning electron microscopy, measurement of dysfunctional mitochondria, ROS accumulation, S cell arrest and apoptotic pathway.ResultsA single-chain variable-fragment phage (JM) with high affinity to MP65 was screened from human single-fold single-chain variable-fragment libraries and designed as a binding target for C. albicans cells. Subsequently, PPa was integrated into JM phage to generate a combined nanoscale material, which was called PPA-JM-phage. After photodynamic inactivation, the growth of C. albicans was inhibited by PPA-JM-phage and apoptosis was observed. Scanning electron microscopy analysis revealed shrinking and rupturing of C. albicans. We also found that depolarization of mitochondrial membrane potential was decreased and intracellular reactive oxygen species levels were elevated significantly in C. albicans inhibited by PPA-JM-phage. Additionally, PPA-JM-phage also lead to S-phase arrest, and metacaspase activation resulting from mitochondrial dysfunction was also found to be involved in C. albicans apoptosis.ConclusionPPa-JM-phage may induce C. albicans apoptosis through a caspase-dependent pathway and the results herein shed light on the potential application of phtototherapeutic nanostructures in fungal inactivation.

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Bionanoparticles in cancer imaging, diagnosis, and treatment

Bao

Yang

et al. 2022

VIEW

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Biological molecules such as DNA, proteins, and lipids can be assembled into naturally existing nanoparticles, such as bacterial viruses (also called bacteriophages or phages), plant viruses, nucleic acid nanoparticles (e.g., DNA origami), protein nanoparticles, and exosomes. These bionanoparticles have their own distinct properties (including compositions, structures, shapes, and functions), laying the foundation for their unique applications as probes for cancer imaging, as detectors for cancer diagnosis, or as therapeutics for cancer therapy. To highlight how the distinct properties of different bionanoparticles can be explored in cancer nanotheranostics, this review critically analyzed the use of bionanoparticles in cancer imaging, diagnosis, and treatment. Specifically, for each of these representative bionanoparticles, we describe its unique properties that render it powerful in cancer theranostics compared with synthetic inorganic nanoparticles. We also summarize how to genetically or chemically modify or redesign the bionanoparticles so that they gain new functions desired for cancer theranostics, such as tumor‐seeking or tumor‐destructive capabilities. Finally, we discussed the challenges in this exciting field. The bionanoparticles covered in this review represent different biomolecular assemblies with unique theranostic applications, showcasing the power of bionanoparticles in disease diagnosis and treatment.

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Novel nanoscale bacteriophage-based single-domain antibodies for the therapy of systemic infection caused by Candida albicans

Cited by 10 publications

References 43 publications

Phage nanofibers in nanomedicine: Biopanning for early diagnosis, targeted therapy, and proteomics analysis

Phage nanofibers in nanomedicine: Biopanning for early diagnosis, targeted therapy, and proteomics analysis

Difunctional bacteriophage conjugated with photosensitizers for <em>Candida albicans</em>-targeting photodynamic inactivation

Bionanoparticles in cancer imaging, diagnosis, and treatment

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