Novel nanohybrid biocatalyst: application in the kinetic resolution of secondary alcohols

Galvão, Wesley S.; Pinheiro, Bruna B.; Golçalves, L. R. B.; Mattos, Marcos Carlos de; Fonseca, T. S.; Regis, T.; Zampieri, Dávila; Santos, José Cleiton Sousa dos; Costa, Luelc Souza da; Corrêa, M.A.; Bohn, F.; Fechine, Pierre Basílio Almeida

doi:10.1007/s10853-018-2641-5

Cited by 130 publications

(37 citation statements)

References 84 publications

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“…Immobilization improves enzyme stability, activity, reduces the inhibition by reaction products, enables continuous production, and also allows biocatalyst removal from product stream [5,6,7]. Although there are many researchers reporting enzyme immobilization techniques, only a few have indeed been commercialized because immobilization costs are usually very high [6].…”

Section: Introductionmentioning

confidence: 99%

Use of Yarrowia lipolytica Lipase Immobilized in Cell Debris for the Production of Lipolyzed Milk Fat (LMF)

Fraga

Penha

Pereira

et al. 2018

IJMS

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Lipase immobilized on Yarrowia lipolytica cell debris after sonication of yeast cells (LipImDebri) was used in hydrolysis reaction as a novel strategy to produce lipolyzed milk fat (LMF). Extracellular (4732.1 U/L), intracellular (130.0 U/g), and cell debris (181.0 U/g) lipases were obtained in a 4 L bioreactor using residual frying oil as inducer in 24 h fermentation process. LipImDebri showed a good operational stability retaining 70% of lipolytic activity after the second cycle and 40% after the fourth. The highest degree of hydrolysis (28%) was obtained with 500 mg LipImDebri for 6 h of lipolysis of anhydrous milk fat. LMF produced with LipImDebri presented high contents of oleic (35.2%), palmitic (25.0%), and stearic (15.4%) acids and considerable amounts of odor-active short and medium chain fatty acids (C:4–C:10) (8.13%).

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Section: Introductionmentioning

confidence: 99%

Use of Yarrowia lipolytica Lipase Immobilized in Cell Debris for the Production of Lipolyzed Milk Fat (LMF)

Fraga

Penha

Pereira

et al. 2018

IJMS

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“…In this direction, the adequate selection of a carrier and the protocol is critical for enzyme immobilization. Different characteristics of carrier materials may critically influence the process of enzyme immobilization, including internal geometry, specific surface area, superficial activation degree, mechanical resistance, and pore size 11,13 . Immobilization via covalent bonding has been proved as a powerful tool to improve enzyme rigidity and catalytic stability 13,14 .…”

Section: Introductionmentioning

confidence: 99%

Effect of shaking speed on immobilization of cephalosporin C acylase: Correlation between protein distribution and properties of the immobilized enzymes

Liu

Tong

Sun

et al. 2020

Biotechnology Progress

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During enzyme immobilization, enzyme activity and protein distribution are affected by various factors such as enzyme load, temperature, and pH. In general, two types of protein distribution patterns (heterogeneous or homogeneous) are observed inside a porous carrier, owing to differences in preparation parameters. During the immobilization of a fusion protein (CCApH) of cephalosporin C acylase (CCA) and pHluorin (a pH-sensitive mutant of green fluorescent protein), different shaking speeds induced obvious differences in protein distribution on an epoxy carrier, LX-1000EPC. Enzyme immobilization with a homogeneous distribution pattern was observed at a low shaking speed (120 rpm) with an operational stability of 10 batches at 37 C. The operational stability of an immobilisate with heterogeneous protein distribution prepared at a high shaking speed (200 rpm) was six batches. Given the pH-sensitive characteristics of pHluorin in the fusion protein, the intraparticle pH of CCApH immobilisates during catalysis was monitored using confocal laser scanning microscopy. The microenvironmental pH of the immobilisate with heterogeneous protein distribution sharply decreased by about 2 units; this decrease in the pH may be detrimental to the lifespan of immobilized CCA. Thus, this work demonstrates the good operational stability of pH-sensitive proton-forming immobilized enzymes with homogeneous protein distribution.

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“…Chitin is another nano‐additive that is commonly used to enhance the physical properties of polymers, due to its low‐cost, excellent biocompatibility, biodegradability, and antibacterial properties . Nano‐sized chitin has been successfully used in the preparation of composites with PLA, poly(caprolactone), poly(vinyl alcohol), chitosan, starch, and natural rubber . Surprisingly, the synthesis and functionality of PBAT/nanochitin composites have received minimal attention to date, motivating a detailed investigation.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable Poly(butylene adipate‐co‐terephthalate) composites reinforced with bio‐based nanochitin: Preparation, enhanced mechanical and thermal properties

Meng

Xie

Waterhouse

et al. 2019

J of Applied Polymer Sci

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The accumulation of nonbiodegradable petrochemical-based polymers in the environment motivates the development and use of low-cost, eco-friendly, and biodegradable polymers. A series of biodegradable poly(butylene adipate-co-terephthalate) composites reinforced by sustainably sourced nanochitin were successfully prepared using melt blending and compression molding methods. Structural, thermal, and mechanical characterizations of poly(butylene adipate-co-terephthalate) (PBAT)/nanochitin composites were performed. SEM revealed that the nanochitin was uniformly dispersed throughout the PBAT matrix at low contents (<2 wt %), while DSC analyses revealed a corresponding increase in the crystallinity (32.6% enhancement) of the PBAT matrix. The tensile strength and elongation at break of the PBAT/nanochitin composite containing 0.5 wt % nanochitin were higher by 82.5 and 64.2%, respectively, compared with pristine PBAT. The Chitin-0.5 composite also showed improved thermal stability compared with PBAT (the char yield improved by 8%) due to the uniform dispersion of nanochitin in the PBAT matrix. The enhanced performance of the PBAT/nanochitin composites, prepared without an added compatibilizer, informs the development of improved biodegradable PBATbased polymers.

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Novel nanohybrid biocatalyst: application in the kinetic resolution of secondary alcohols

Cited by 130 publications

References 84 publications

Use of Yarrowia lipolytica Lipase Immobilized in Cell Debris for the Production of Lipolyzed Milk Fat (LMF)

Use of Yarrowia lipolytica Lipase Immobilized in Cell Debris for the Production of Lipolyzed Milk Fat (LMF)

Effect of shaking speed on immobilization of cephalosporin C acylase: Correlation between protein distribution and properties of the immobilized enzymes

Biodegradable Poly(butylene adipate‐co‐terephthalate) composites reinforced with bio‐based nanochitin: Preparation, enhanced mechanical and thermal properties

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