Novel Nanodimension artificial red blood cells that act as O₂and CO₂carrier with enhanced antioxidant activity: PLA-PEG nanoencapsulated PolySFHb-superoxide dismutase-catalase-carbonic anhydrase

Abstract

Poly(ethylene glycol)-Poly(lactic acid) block-copolymer (PEG-PLA) was prepared and characterized using Fourier transform infrared spectrophotometer (FTIR). Glutaraldehyde was used to crosslink stroma-free hemoglobin (SFHb), superoxide dismutase (SOD), catalase (CAT), and carbonic anhydrase (CA) into a soluble complex of PolySFHb-SOD-CAT-CA. PEG-PLA was then used to nanoencapsulated PolySFHb-SOD-CAT-CA by oil in water emulsification. This resulted in the formation of PLA-PEG-PolySFHb-SOD-CAT-CA nanocapsules that have enhanced antioxidant activity and that can transport both O 2 and CO 2 . These are homogeneous particles with an average diameter of 100 nm with good dispersion and core shell structure, high entrapment efficiency (EE%), and nanocapsule percent recovery. A lethal hemorrhagic shock model in rats was used to evaluate the therapeutic effect of the PLA-PEG-PolySFHb-SOD-CAT-CA nanocapsules. Infusion of this preparation resulted in the lowering of the elevated tissue PCO 2 and also recovery of the mean arterial pressure (MAP).

Development of novel delivery system for nanoencapsulation of catalase: formulation, characterization, and in vivo evaluation using oxidative skin injury model

“…Similarly, an increase in enzyme liposomal entrapment efficiency occurred with increasing the initial enzyme concentration up to a certain limit followed by a decrease in EE [17,34]. It was previously reported that catalase activity was stabilized upon incorporation into vesicles [35], similarly catalase antioxidant activity was enhanced upon nano-encapsulation [36,37].…”

A novel amperometric catechol biosensor based on α-Fe2O3 nanocrystals-modified carbon paste electrode

“…Further, the laccase enzyme was immobilized along with stabilizing and cross-linking agents such as BSA and glutaraldehyde and packed in Teflon tube to fabricate amperometric biosensors. Bifunctional agents such as glutaraldehyde cross-link with the reactive amino groups of enzymes (Gao et al 2013). The incorporation of BSA along with glutaraldehyde during the process of enzyme immobilization through hydrophobic interactions, contributed to the longterm operational stability of the enzyme biosensor.…”

Preparation and characterization of PEG-modified PCL nanoparticles for oxygen carrier: a new application of Fourier transform infrared spectroscopy for quantitative analysis of the hemoglobin in nanoparticles

“…This result is different from previous study of other PLA-PEG-based HbPN system. It has been reported that (Gao et al 2013), after encapsulating the soluble complex of PolySFHb-SOD-CAT-CA, the PLA-PEG NPs become homogeneous particles with core-shell structure. This phenomenon is mainly due to the absence of the surfactant residue on the surface of the HbPNs.…”