“…Natural polysaccharides are commonly used as stabilisers as to ensure system stability (Shao et al ., ; Yu et al ., ; Zhao et al ., ). Pectin is also a promising drug delivery polymer due to its gelling properties, bioadhesiveness, biocompatibility and non‐toxicity (Willats et al ., ).…”
To improve long-term stability of the liposomes, resveratrol (RES) liposomes, epigallocatechin gallate (EGCG) liposomes, RES+EGCG liposomes, RES+EGCG liposomes coated with low methoxyl pectin (LMP) were prepared, and their physical properties were evaluated. The prepared liposomes have a particle sizes of about 110-160 nm. The LMP-coated composite liposomes have the largest particle size and zeta potential, the latter indicating higher system stability. Liposome systems were pasteurised and placed in an orange juice system as to study liposome stability in a real the food environment. LMP bridge with metal ions forming a network-like gel in the LMP-coated composite liposomes, thereby enhancing the stability of liposomes. LMP-decorated liposomes could be developed as a formulation for encapsulating and delivering functional ingredients.Stability of complex liposomes in orange juice S. Feng et al.
“…Natural polysaccharides are commonly used as stabilisers as to ensure system stability (Shao et al ., ; Yu et al ., ; Zhao et al ., ). Pectin is also a promising drug delivery polymer due to its gelling properties, bioadhesiveness, biocompatibility and non‐toxicity (Willats et al ., ).…”
To improve long-term stability of the liposomes, resveratrol (RES) liposomes, epigallocatechin gallate (EGCG) liposomes, RES+EGCG liposomes, RES+EGCG liposomes coated with low methoxyl pectin (LMP) were prepared, and their physical properties were evaluated. The prepared liposomes have a particle sizes of about 110-160 nm. The LMP-coated composite liposomes have the largest particle size and zeta potential, the latter indicating higher system stability. Liposome systems were pasteurised and placed in an orange juice system as to study liposome stability in a real the food environment. LMP bridge with metal ions forming a network-like gel in the LMP-coated composite liposomes, thereby enhancing the stability of liposomes. LMP-decorated liposomes could be developed as a formulation for encapsulating and delivering functional ingredients.Stability of complex liposomes in orange juice S. Feng et al.
“…The data from phenol-sulfuric acid method and the Bradford reaction confirmed 80.3% sugar and 5.7% protein, indicating that EPS-160 was mainly composed of polysaccharides. These results agreed with the published reports that the dominant components of EPS were carbohydrates and protein with 75-90% percent (More et al, 2014;Yin et al, 2014;Zhao et al, 2017;Guo et al, 2018). EPS-160 was hydrolyzed with TFA to measure the monosaccharide composition.…”
Section: Characterization Of Eps-160supporting
confidence: 90%
“…In the case of Klebsiella sp. PHRC 1.001, high exopolysaccharide production with excellent emulsifying properties was 21 g/L in a 7 L fermentor for 3 days (Zhao et al, 2017). Additionally, the production time was generally between 2 and 4 days in the handful of other genus strains with hyperproduction of bioflocculants.…”
Section: Improvement Of Eps-160 Production By Rsmmentioning
“…However, the microbial genus in low-water content oil wells affect the oil recovery, among Chromohalobacter can oxidize and convert into sulfides in water to form elemental sulfur or sulfate 30 . Klebsiella can metabolize acetone, butanol, and 2-hydroxypropane with these metabolites and thereby improve the permeability of carbonate rocks while reducing the viscosity of crude oil 31 . Most Marinobacter were described as halophilic oil-utilizing and biosurfactant-producing bacteria, which played a key role in enhancing oil recovery by degrading naphthenes and PAHs (especially phenanthrene and thiophene) under anaerobic and high salinity conditions 20 .…”
Section: Resultsmentioning
confidence: 99%
“…Further analysis of sequencing and crude oil biotreatment results showed that functional microbial groups can form a symbiotic or alternate system under the optimum ratio of oil-water environment that provide growth factors, metabolic stimuli, or metabolic intermediates for the growth of functional bacteria. From what has been discussed above, mechanism pathways of the synergistic of functional microbes in crude oil biotreatment under different water cut conditions in low permeability reservoirs are as follows: (1) reduce the interfacial tension of oil-water and increase the crude oil dispersion 12,46 ; (2) promote the emulsification and dispersion of crude oil, and reduce the crude oil migration resistance 31,47 ; (3) dissolve in crude oil to reduce the viscosity and provide the metabolic substrates for other microorganisms 13,42 ; (4) catalytic the crude oil biodegradation and increase the contact area 48 ; (5) change the wettability of rock surface and increase the peeling ability of crude oil 41 ; (6) increase formation pressure and improve permeability 42 ; (7) increase the capacity of adsorption and metabolism of functional microbes 20 .Figure 7Mechanism pathways establishing the synergistic of functional microbial groups in crude oil biotreatment under different water cut conditions. The labeled species were the most abundant in each functional microbial group.…”
Low permeability oil reservoirs are a widespread petroleum reservoir type all over the world. Therefore, methods to recover these reservoirs efficiently are of importance to guarantee energy supply. Here we report our novel stimulation of indigenous microbes by optimizing the water cut in low permeability reservoirs for green and enhanced oil recovery. We aimed to investigate the characteristics of indigenous bacterial communities with changes in water cut in reservoirs by high-throughput sequencing technology, and reveal the mechanism and characteristics of the crude oil biotreatment under different crude oil-water ratio conditions and the optimum activation time of indigenous functional microbial groups in reservoirs. The indigenous microbial metabolism products were characterized by gas chromatography mass spectrometry. Results showed that Acinetobacter (47.1%) and Pseudomones (19.8%) were the main functional genus of crude oil degradation at the optimal activation time, and can reduce the viscosity of crude oil from 8.33 to 5.75 mPa·s. The dominant bacteria genus for oil recovery after activation of the production fluids was similar to those in the reservoirs with water cut of 60–80%. Furthermore seven mechanism pathways of enhancing oil recovery by the synergistic of functional microbial groups and their metabolites under different water cut conditions in low permeability reservoirs have been established.
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