Novel mutations and polymorphisms in the human dystrophin gene detected by double‐strand conformation analysis

Saad, Fawzy A.; Mostacciuolo, Maria Luisa; Trevisan, Carlo P.; Tomelleri, Giuliano; Angelini, C.; Salam, Ekram Abdel; Danieli, Gian Antonio

doi:10.1002/(sici)1098-1004(1997)9:2<188::aid-humu15>3.3.co;2-j

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“…A fraction of the ligation reaction was used to transform DH5α bacterial competent cells. The plasmid DNA was extracted using a microextraction procedure described elsewhere [Saad et al, 1997]. Recombinant clones were selected by PCR using primers originating from the vector sequences.…”

Section: Methodsmentioning

confidence: 99%

Identification of osteopontin phosphorylation sites involved in bone remodeling and inhibition of pathological calcification

Saad

Salih

Glimcher

2007

J of Cellular Biochemistry

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Osteopontin is a noncollagenous, phosphorylated extracellular glycoprotein, expressed in mineralized and nonmineralized tissues, organs and body fluids. The protein contains an RGD tripeptide cell-binding motif, and is subjected to a variety of posttranslational modifications that play important roles in its multiple biological functions, such as bone remodeling and inhibition of pathological calcification. In this study, we have expressed bovine osteopontin in a prokaryotic system and identified the seven amino acid residues phosphorylated in vitro by CKII.Keywords osteopontin phosphorylation; casein kinase II OPN cDNA sequences have been determined and patterns of expression characterized in several species. OPN contains an arginine-glycine-aspartate (RGD) cell-binding tripeptide [Oldberg et al., 1986] and regions with an elevated level of aspartic acid. There are tissue specific differences of OPN not only in protein expression levels but also in the extent of phosphorylation. Osteopontin in the mineralized extracellular matrix of bone tissue [Prince et al., 1987] and milk [Sorensen et al., 1995] is highly phosphorylated and rat kidney cells secrete both phosphorylated and non-phosphorylated forms of the protein [Singh et al., 1990]. A number of experiments have reported as many as 10 residues of phosphorylated amino acid residues in chicken bone and as many as 28 phosphoresidues in bovine milk osteopontin [Sorensen et al., 1995;Salih et al., 1996a]. Recent analysis of OPN-deficient mice also showed impaired angiogenesis, early callus formation, and late stage remodeling in fracture healing [Duvall et al., 2007]. Several in vivo biological functions of OPN have been postulated [Chen et al., 1993;Weber and Cantor, 1996;Oates et al., 1997;Liaw et al., 1998;Ashkar et al., 2000;Rittling and Chambers, 2004;Duvall et al., 2007;Mori et al., 2007]. For example, the pathological calcification of arteries and cartilage in mice in which matrix gla protein has been deleted [Luo et al., 1997] increases when OPN is also deleted [Speer et al., 2002]. OPNdeficient mice also develop pathological calcification in vascular smooth muscle cells [Ohri et al., 2005]. The inhibition of these instances of pathological calcification has been attributed to the presence of the casein kinase II (CKII) phosphorylated residues in OPN [Jono et al., 2000]. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author ManuscriptThe binding of Ca ions by osteopontin [Chen et al., 1992] may explain its inhibition of apatite crystal nucleation when added to metastable solutions of Ca 2+ and inorganic phosphate [Hunter et al., 1996;Pampena et al., 2004].Since CKII has been identified as a major kinase synthesized in bone during organ and cell culture [Mikuni-Takagaki and Glimcher, 1990;Salih et al., 1996a;Sfeir and Veis, 1996;Wang et al., 1998], we used CKII to phosphorylate recombinant OPN and to identify the specific phosphorylated residues that may play a role in some of these biological functions. We note, however, that no significa...

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Section: Methodsmentioning

confidence: 99%

Identification of osteopontin phosphorylation sites involved in bone remodeling and inhibition of pathological calcification

Saad

Salih

Glimcher

2007

J of Cellular Biochemistry

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“…Screening for point mutations was performed by Double Strand Conformation Analysis (DSCA) [Saad et al, 1994]. Briefly, leukocyte genomic DNA was amplified by polymerase chain reaction (PCR), a fraction of PCR products loaded onto vertical slab polyacrylamide gel, and then gels were silver stained according to procedures described elsewhere [Saad et al, 1997].…”

Section: Methodsmentioning

confidence: 99%

“…For each amplicon showing electrophoretic mobility shift, the products of five independent PCR amplifications were pooled and an aliquot of this mixture was used for cloning the sequence in pCRII vector (Invitrogen). The insert orientation deduction and generation of biotinylated PCR products were as described [Saad et al, 1997].…”

Section: Methodsmentioning

confidence: 99%

Double missense mutation in exon 41 of the human dystrophin gene detected by double strand conformation analysis

et al. 1998

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Development of late-onset Becker muscular dystrophy is reported in a patient whose two healthy brothers showed high serum creatine kinase level. No cases of neuromuscular disorders had been previously reported in this family. The analysis of the dystrophin gene showed that the three brothers had A-->C transversion at nucleotide 6092 in exon 41, a missense mutation which converts lysine into glutamine. The symptomatic patient showed an additional mutation in the same exon, a T-->C transition at nucleotide 6119, converting a phenylalanine to leucine. The possible pathogenic role of this mutation is discussed.

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Novel mutations and polymorphisms in the human dystrophin gene detected by double‐strand conformation analysis

Cited by 2 publications

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Identification of osteopontin phosphorylation sites involved in bone remodeling and inhibition of pathological calcification

Identification of osteopontin phosphorylation sites involved in bone remodeling and inhibition of pathological calcification

Double missense mutation in exon 41 of the human dystrophin gene detected by double strand conformation analysis

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