Novel mutation method for increased cellulase production

Chand, Parkash; Aruna, Ajithadas; Maqsood, Ambreen; Rao, L. Venkateswar

doi:10.1111/j.1365-2672.2004.02453.x

Cited by 118 publications

(72 citation statements)

References 24 publications

(19 reference statements)

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“…Furthermore, Dhawan et al (2003) reported that growing fungus Cyathus bulleri mutated by phenanthridine dye ethidium bromide could produce a 4.2-fold increase in laccase production as compared to the untreated fungus. Our results, combined with those of others including Chand et al (2005) and Dhawan et al (2003), have shown that the fungal spores or mycelia grown with mutagens can be an effective method for enhancing the objective products' production.…”

Section: Discussionmentioning

confidence: 77%

“…However, some reports showed that subjecting the dormant spores to microwave or ultraviolet light irradiation could not obtain obvious results (Anwar et al 1996;Xia et al 2007). While Chand et al (2005) reported that germinating spores of Aspergillus and Trichoderma species treated with ethidium bromide and ultraviolet light were effective methods for improving cellulase production. Furthermore, Dhawan et al (2003) reported that growing fungus Cyathus bulleri mutated by phenanthridine dye ethidium bromide could produce a 4.2-fold increase in laccase production as compared to the untreated fungus.…”

Section: Discussionmentioning

confidence: 99%

“…After incubated in a rotary shaker at 150 rpm and 26°C for 8 h, a 20-ml sample of germinating spore suspension was treated with microwave in a microwave oven (frequency, 2,450 MHz; power, 650 W) for 90 s and then irradiated with ultraviolet light (wavelength, 253.7 nm; power, 30 W; distance, 30 cm) for 30 min (Chand et al 2005;Xia et al 2007). Subsequently, the treated spore suspension was plated in PDA plates and incubated at 26°C for 5 days.…”

Section: Microorganism Random Mutagenesis and Mutant Screeningmentioning

confidence: 99%

See 2 more Smart Citations

Enhanced production of a novel cytotoxic chromone oxalicumone A by marine-derived mutant Penicillium oxalicum SCSIO 24–2

Zhang

Bao

Zhong

et al. 2013

Appl Microbiol Biotechnol

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Many marine natural products hold great potential for the development of new and much needed drugs. However, the production of active metabolites by marine-derived microorganisms is usually very low, and large-scale culture has to be involved to meet the need of chemical structural modification and deep pharmacy study. In order to enhance the production of a novel cytotoxic sulfur-containing chromone oxalicumone A (OA), germinating spores of a marine-derived wild strain Penicillium oxalicum SCSGAF 0023 were mutated by microwave and ultraviolet light irradiation, which led to the obtainment of a mutant P. oxalicum SCSIO 24-2 that could produce fivefold increase in OA production (3.42±0.21 mg/l) as compared to the wild strain. This is the first report that germinating spores are applied in marine-derived Penicillium sp. mutating to enhance the production of OA. Further, Plackett-Burman design and central composite design were adopted to optimize the basic medium components for increasing OA production by the mutant SCSIO 24-2 in shake flasks. The results indicated that three medium components including mannitol, maltose, and L -cysteine had significant effects on OA production, and their concentrations were optimized as 36, 27.9, and 0.99 g/l, respectively. In the optimized medium, the OA production (18.31±0.27 mg/l) by mutant SCSIO 24-2 was 4.4-fold higher than that in the basic medium. These results of this work promise to improve the present production of OA and may be adopted to enhance other objective products' production by marine-derived fungi.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Microorganism Random Mutagenesis and Mutant Screeningmentioning

confidence: 99%

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Enhanced production of a novel cytotoxic chromone oxalicumone A by marine-derived mutant Penicillium oxalicum SCSIO 24–2

Zhang

Bao

Zhong

et al. 2013

Appl Microbiol Biotechnol

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“…Jiang et al (2011) observed CMCase activity in T. viride mutants reaching 18 U ml -1 , which was also three times higher than in the wild type strain. Chand et al (2005) measured about 0.415 and 0.60 U ml -1 in endoglucanase activity in Aspergillus mutants compared to 0.280 U ml -1 in the wild type strain. Another study tested Penicillium echinulatum using various cellulosic substrates and detected the maximum CMCase activity as 1.53 U ml -1 (Martins et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Many traditional mutagenic strategies have been used to improve this characteristic, nevertheless, these attempts have not been totally successful yet (Chand et al, 2005;Kovàcz et al, 2008;Kovàcz et al, 2009;Jiang et al, 2011), since current enzyme production involves high costs and the production process is not yet fully defined (Adsul et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Production of thermophilic and acidophilic endoglucanases by mutant Trichoderma atroviride 102C1 using agro-industrial by-products

Oliveira¹,

Lima²,

Bon³

et al. 2016

Afr. J. Biotechnol.

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Many traditional mutagenic strategies have been used to improve cellulase production by microorganisms, especially fungi species. Trichoderma species are among cellulolytic fungi, those that have been most extensively studied, due to their efficient production of these enzymes. In the present study, N-methyl-N´-nitro-N-nitrosoguanidine (NTG) was used as mutagenic agent to obtain cellulolytic mutant from wild strain T. atroviride 676. After mutagenic procedures, two strains (102C1 and 104C2) were selected as promising cellulase-producing mutant. The effect of the carbon (sugarcane bagasse: SCB) and nitrogen (corn steep liquor: CSL) sources on endoglucanase production by the mutants 102C1 and 104C2 was studied using submerged cultivations at 28°C. Different concentrations of SCB and CSL were used and nine different media were generated. Mutant 102C1 showed the best results when using 2.5% SCB and 0.7% CSL. A central composite rotational design (CCRD) was performed to estimate optimal conditions of pH and temperature for endoglucanase activity of strain 102C1, which were pH 3.6 and temperature 66°C. The characterization of this acidophilic and thermophilic endoglucanase activity produced by the mutant strain 102C1 allows its use in biotechnological applications, particularly in the hydrolysis of agro industrial residues, such as SCB, for bioethanol production.

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Comprehensive studies on optimization of cellulase and xylanase production by a local indigenous fungus strain via solid state fermentation using oil palm frond as substrate

Tai

Tan

Lim

et al. 2019

Biotechnology Progress

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The high cost of cellulases remains the most significant barrier to the economical production of bio-ethanol from lignocellulosic biomass. The goal of this study was to optimize cellulases and xylanase production by a local indigenous fungus strain (Aspergillus niger DWA8) using agricultural waste (oil palm frond [OPF]) as substrate.The enzyme production profile before optimization indicated that the highest carboxymethyl cellulose (CMCase), filter paper (FPase), and xylanase activities of 1.06 U/g, 2.55 U/g, and 2.93 U/g were obtained on day 5, day 4, and day 5 of fermentation, respectively. Response surface methodology was used to study the effects of several key process parameters in order to optimize cellulase production. Of the five physical and two chemical factors tested, only moisture content of 75% (w/w) and substrate amount of 2.5 g had statistically significant effect on enzymes production. Under optimized conditions of 2.5 g of substrate, 75% (w/w) moisture content, initial medium of pH 4.5, 1 × 10 6 spores/mL of inoculum, and incubation at ambient temperature (±30 C) without additional carbon and nitrogen, the highest CMCase, FPase, and xylanase activities obtained were 2.38 U/g, 2.47 U/g, and 5.23 U/g, respectively. Thus, the optimization process increased CMCase and xylanase production by 124.5 and 78.5%, respectively. Moreover, A. niger DWA8 produced reasonably good cellulase and xylanase titers using OPF as the substrate when compared with previous researcher finding. The enzymes produced by this process could be further use to hydrolyze biomass to generate reducing sugars, which are the feedstock for bioethanol production. K E Y W O R D SAspergillus niger, cellulases, oil palm frond, response surface methodology, xylanase

show abstract

Novel mutation method for increased cellulase production

Abstract: This new method could be applied to obtain potent fungal mutants for more enzymes production.

Cited by 118 publications

References 24 publications

Enhanced production of a novel cytotoxic chromone oxalicumone A by marine-derived mutant Penicillium oxalicum SCSIO 24–2

Enhanced production of a novel cytotoxic chromone oxalicumone A by marine-derived mutant Penicillium oxalicum SCSIO 24–2

Production of thermophilic and acidophilic endoglucanases by mutant Trichoderma atroviride 102C1 using agro-industrial by-products

Comprehensive studies on optimization of cellulase and xylanase production by a local indigenous fungus strain via solid state fermentation using oil palm frond as substrate

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