Novel multiplex PCR for the detection of the Staphylococcus aureus superantigen and its application to raw meat isolates in Korea

Prevalence and Distribution of Superantigen Toxin Genes in Clinical Community Isolates of Staphylococcus Aureus

“…Both genes belong to the recently described enterotoxin gene cluster (egc) that harbours 5 to 6 genes (seg, sei, selm, seln, selo, and sometimes selu), which cluster on a staphylococcal pathogenicity island type I γSaβ [33,36,37], this cluster will be found without all of the egc components [33,[38][39][40]. Furthermore, many studies reported that some isolates contained selo gene with sed, selj and ser genes instead of the egc genes [40][41][42]; in fact, Thomas et al [42] suggested that this phenomenon is caused when a part of egc is exported or inserted in the chromosome of other S. aureus strains intermediated by insertion sequences.…”

Selection of optimal combinations of loci by the Optimal Combination Finder computer program from a group of variable number tandem repeat loci for use in Staphylococcus aureus food poisoning case investigations

“…The profile of the other isolate from patient 1 (00M1063-2) was the same as that of isolate Chicken salad P252B00 sample B. In addition, all isolates in this case investigation had the same four enterotoxin genes, sea, sek, seh and seq, out of the 19 enterotoxin genes tested for (Hwang et al, 2007). The MLVA profiles of isolates from the suspected food matched the profiles of isolates from patients, suggesting that the food contained different populations of S. aureus and subsequently the two different genotypes of the isolates were reflected in patient isolates.…”

“…Detection of 19 enterotoxin genes was carried out for these outbreak isolates according to the method described by Hwang et al (2007).…”

Detecção de genes do cluster egc em Staphylococcus aureus isolados de alimentos de origem animal

“…A relação entre a presença do cluster egc e patogenicidade tem sido descrita em cepas de S. aureus isoladas de fontes clínicas (van BELKUM et al, 2006) entretanto, com cepas isoladas em alimentos, o número de trabalhos é bastante reduzido (BANIA et al, 2006;HWANG et al, 2007;LAWRYNOWICZ-PACIOREK et al, 2007). Dessa forma, os objetivos foram avaliar a presença desse cluster em S. aureus isolados em Cinco cepas albergavam um ou mais genes do cluster, mas não o egc completo.…”

“…A heterogeneidade genética observada pode ser explicada pela necessidade de a bactéria portar determinados genes, os quais favoreceriam seu Tabela 2 -Distribuição e porcentagem dos diferentes genótipos do cluster egc em S. aureus isolados em alimentos de origem animal na cidade de Pelotas, RS. (NITZSCHE et al, 2007;HWANG et al, 2007).…”