Novel multifunctional PB and PBH hydrogels as soft filler for tissue engineering

Abstract

In this study, we designed multifunctionalized hydrogel scaffolds and injectable particles based on highmolecular-weight (M W ) pullulan and human-like collagen (HLC) crosslinked with 1,4-butanediol diglycidyl ether (BDDE) for combination therapy tissue restoration. The properties of the pullulan/BDDE (PB) and pullulan/BDDE/human-like collagen (PBH) hydrogels were characterized via swelling ratio measurements, mechanical tests, and enzymatic degradation in vitro and via subcutaneous injections in vivo. The results demonstrate that the dry hydrogels completely returned to their original state in deionized water. The elastic modulus of the PBH53 dry hydrogels is higher than that of the other hydrogels after exposure to bending stress and compression stress with a maximum value of 7858.93 MPa. In addition, the in vitro live/dead staining and cell adhesion of the PBH hydrogels exhibited a superior fibroblast morphology without high levels of cell death, which were considerably better than those of PB hydrogels. In vivo, PB and PBH particles with good biocompatibility and anti-biodegradation were successfully prepared via the granulation of wet PB and PBH hydrogels for efficient subcutaneous injection in Kunming mice and New Zealand rabbits. Therefore, the PB and PBH hydrogels were found to be acceptable, safe, soft materials for use in skin restoration, cartilage treatment, and lacrimal dryness therapy. Fig. 3 Cross-sections of the PB and PBH hydrogels before and after degradation by pullulanase, collagenase I and pullulan/collagenase I, as observed by SEM. The first line denotes the cross-section for the four hydrogels (50Â), with a scale bar of 400 mm; the second line represents the cross-section for the four hydrogels at a higher magnification (100Â), with a scale bar of 200 mm; the third line represents the particles for the four hydrogels (100Â), with a scale bar of 200 mm; the fourth line denotes the cross-section for the four hydrogels after degradation by pullulanase (100Â), with a scale bar of 200 mm; the fifth line represents the cross-section for the four hydrogels after degradation by collagenase I (100Â), with a scale bar of 200 mm; and the sixth line denotes the cross-section for the four hydrogels after degradation by pullulanase/collagenase I (250Â), with a scale bar of 100 mm.Fig. 6 Cell adhesion adherent cells on the hydrogels. SEM images of fibroblast attachment on the PB10 hydrogel (A1-A3), PBH10 hydrogel (B1-B3), PB53 hydrogel (C1-C3) and PBH53 hydrogel (D1-D3) at 24 (A1, B1, C1 and D1), 48 (A2, B2, C2 and D2) and 72 h (A3, B3, C3 and D3); the scale bar is 10 mm. Journal of Materials Chemistry B Paper

A Novel Human-Like Collagen Hydrogel Scaffold with Porous Structure and Sponge-Like Properties

“…Due to these properties, HLC is considered a promising biomaterial. HLC’s ability to be applied in all aspects of biomedical engineering have been researched, including as a soft tissue filler [ 28 ], a hemostatic sponge [ 29 , 30 ], and a vascular scaffold [ 31 ].…”

Thermo-Sensitive Nanomaterials: Recent Advance in Synthesis and Biomedical Applications

“…The system was able to continuously release oxygen for four weeks leading to a significant increase in cardiac function of infarcted rats. Furthermore, different kinds of hydrogels have been developed for skin wound dressings [ 152 , 153 , 154 ].…”

Preparation and characterization of a supramolecular hydrogel made of phospholipids and oleic acid with a high water content

“…[5][6][7][8] These compounds assemble in water by either physical or chemical interactions to form gels with potential applications in drug delivery, tissue engineering or cell culturing. [9][10][11][12] Common alternatives to these systems, especially for drug delivery applications, are the ones formed by phospholipids. These molecules can aggregate in water by hydrophobic forces and electrostatic attractions giving rise to structures of different morphologies and phase behaviors.…”