Novel Multifunctional Meta‐Surface Plasmon Resonance Chip Microplate for High‐Throughput Molecular Screening

Abstract

The utilization of surface plasmon resonance (SPR) sensors for real‐time label‐free molecular interaction analysis is already being employed in the fields of in vitro diagnostics and biomedicine. However, the widespread application of SPR technology is hindered by its limited detection throughput and high cost. To address this issue, our study introduces a novel multifunctional MetaSPR high‐throughput microplate biosensor featuring 3D nanocup microarrays, aiming to achieve high‐throughput screening at a reduced cost and with enhanced speed. Different types of MetaSPR sensors and analytical detection methods have been developed for accurate subtype identification, epitope binding, affinity determination, antibody collocation, and other applications that have greatly promoted the screening and analysis of early antibody drugs. The MetaSPR platform combined with nano‐enhanced particles amplified the detection signal and improved the detection sensitivity, making it more convenient, sensitive, and efficient than traditional ELISA. Our findings demonstrated that the MetaSPR biosensor is a new practical technology detection platform that can improve the efficiency of biomolecular interaction studies with unlimited potential for new drug development.This article is protected by copyright. All rights reserved

Application and Method of Surface Plasmon Resonance Technology in the Preparation and Characterization of Biomedical Nanoparticle Materials

“…In summary, SPR technology holds significant promise in drug development and screening due to its real-time, label-free analysis of molecular interactions. 18 , 60 SPR facilitates the rapid high-throughput screening of extensive libraries of drug candidates by immobilizing target molecules on the sensor surface and measuring binding interactions simultaneously, thus accelerating early-stage drug discovery. 18 , 90 This technology also provides detailed kinetic information for understanding binding mechanisms and selecting candidates with optimal characteristics.…”

“… 18 , 60 SPR facilitates the rapid high-throughput screening of extensive libraries of drug candidates by immobilizing target molecules on the sensor surface and measuring binding interactions simultaneously, thus accelerating early-stage drug discovery. 18 , 90 This technology also provides detailed kinetic information for understanding binding mechanisms and selecting candidates with optimal characteristics. 91 The real-time monitoring capability of SPR allows researchers to observe dynamic processes and transient interactions that might be missed by endpoint assays, providing valuable insights into the temporal aspects of drug binding and efficacy.…”

“…The integration of nanomaterial-enhanced particles amplifies detection signals, significantly improving sensitivity and making the process more convenient, sensitive, and efficient than traditional ELISA. 18 Additionally, incorporating two-dimensional materials such as graphene, transition metal dichalcogenides (TMDCs), MXene, black phosphorus (BP), metal-organic frameworks (MOFs), and antimonene into sensors can substantially enhance their sensitivity and detection performance. 19–21 The use of optical fiber sensing probes with PAN nanofiber membranes and gold nanofilm composite sensitive membranes allows for high sensitivity and selectivity in detection.…”