Novel multi-strain probiotics reduces Pasteurella multocida induced fowl cholera mortality in broilers

Abstract

Pasteurella multocida causes fowl cholera, a highly contagious poultry disease of global concern, causing significant ecological and economic challenges to the poultry industry each year. This study evaluated the effects of novel multi-strain probiotics consisting of Lactobacillus plantarum, L. fermentum, Pediococcus acidilactici, Enterococcus faecium and Saccharomyces cerevisiae on growth performance, intestinal microbiota, haemato-biochemical parameters and anti-inflammatory properties on broilers experimentally challenged with P. multocida. A total of 120 birds were fed with a basal diet supplemented with probiotics (108 CFU/kg) and then orally challenged with 108 CFU/mL of P. multocida. Probiotics supplementation significantly (P < 0.05) improved growth performance and feed efficiency as well as reducing (P < 0.05) the population of intestinal P. multocida, enterobacteria, and mortality. Haemato-biochemical parameters including total cholesterol, white blood cells (WBC), proteins, glucose, packed cell volume (PCV) and lymphocytes improved (P < 0.05) among probiotic fed birds when compared with the controls. Transcriptional profiles of anti-inflammatory genes including hypoxia inducible factor 1 alpha (HIF1A), tumor necrosis factor- (TNF) stimulated gene-6 (TSG-6) and prostaglandin E receptor 2 (PTGER2) in the intestinal mucosa were upregulated (P < 0.05) in probiotics fed birds. The dietary inclusion of the novel multi-strain probiotics improves growth performance, feed efficiency and intestinal health while attenuating inflammatory reaction, clinical signs and mortality associated with P. multocida infection in broilers.

Novel mono‐ and multi‐strain probiotics supplementation modulates growth, intestinal microflora composition and haemato‐biochemical parameters in broiler chickens

“…Although previous studies concentrated on mono-strain probiotics, the application of multi-strain probiotics is gradually now reported by many researchers with mixed outcomes (Abdel-Latif et al, 2018;Olnood et al, 2015a). In a recent study, dietary supplementation with multi-strains probiotic consisting of Lactobacillus fermentum, L. plantarum, Enterococcus faecium, Pediococcus acidilactici and Saccharomyces cerevisiae significantly improved growth performance, some haemato-biochemical parameters as well as beneficially modulating gut microflora and also ameliorating Pasteurella multocida infection in broilers (Reuben et al, 2021). Similarly, Fesseha et al (2021), Ramlucken et al (2020), Kazemi et al (2019) and Olnood et al (2015a) separately reported the effects of multi-strain probiotics supplementation on growth performance, gut microbiome development and diversity, intestinal morphology, lipid oxidation and pathogens control in broiler chickens.…”

The Recent Trend in the Use of Multistrain Probiotics in Livestock Production: An Overview

“…Besides, the transcriptional profiles of anti-inflammatory genes in the intestinal mucosa of probioticfed birds were elevated, haemato-biochemical markers such as packed cell volume, total cholesterol, glucose, proteins, white blood cells, and lymphocytes were also improved. There is a possibility that perhaps the synergy between lactic acid bacteria and yeast strains resulted in higher antimicrobial activity against P. multocida and enterobacteria in the guts of supplemented birds, as well as the ability of the combination to out-compete pathogens, thereby preventing them from attaching to the intestinal walls and as a result improve intestinal microbial balance [95].…”

Influence of microbial probiotics on ruminant health and nutrition: sources, mode of action and implications

“…1,140 The findings from several studies revealed that supplementation with probiotics in animals may significantly improve the lipid profile in the blood of ruminants. 141 The supplementation of kids wileyonlinelibrary.com/jsfa or lambs with probiotics was shown to decrease the concentrations of triglycerides, total lipids, low-density lipoproteins and non-esterified fatty acids in the blood of ruminants. 142,143 Panda et al 144 reported a significant reduction in total cholesterol and triglycerides by dietary inclusion of 100 mg kg −1 diet of Lactobacillus sporogenes probiotic in animals.…”

“…The findings from several studies revealed that supplementation with probiotics in animals may significantly improve the lipid profile in the blood of ruminants 141 . The supplementation of kids or lambs with probiotics was shown to decrease the concentrations of triglycerides, total lipids, low‐density lipoproteins and non‐esterified fatty acids in the blood of ruminants 142,143 .…”