Novel mouse model of cardiopulmonary bypass

Madrahimov, N.; Boyle, Erin C.; Gueler, Faikah; Goecke, Tobias; Knöfel, Ann-Kathrin; Irkha, Valentyna; Maegel, Lavinia; Höffler, Klaus; Natanov, Ruslan; Ismail, Imen Ben; Maus, Ulrich A.; Kühn, Christian; Warnecke, G.; Shrestha, M.; Cebotari, Serghei; Haverich, Axel

doi:10.1093/ejcts/ezx237

Cited by 14 publications

(23 citation statements)

References 13 publications

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“…4a). These findings were comparable to our previous data [14], and the lower values were due to hemodilution. Hemoglobin showed a similar drop over the course of the experiments.…”

Section: Hemodynamics and Oxygenation After 4-h Sham And Ecmo Treatmentsupporting

confidence: 92%

Four hours of veno-venous extracorporeal membrane oxygenation using bi-caval cannulation affects kidney function and induces moderate lung damage in a mouse model

Natanov

Khalikov

Gueler

et al. 2019

ICMx

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Background: Improvement of single site cannulation for extracorporeal membrane oxygenation (ECMO) therapy is pivotal for reduction of patient morbidity and mortality in respiratory failure. To further improve the cardiopulmonary outcomes and reduce end organ damage, we established a murine model for single site cannulation with a double lumen cannula. Results: We created a hemodynamically stable double lumen cannula and successfully implanted it through the jugular vein into the upper and lower vena cava. This allowed adequate drainage of the blood. Blood gas analysis showed excellent oxygenation and CO 2 reduction. There was no excessive bleeding. No signs of right heart congestion were present which was confirmed in the histological analysis of the liver. Histology demonstrated moderate lung damage and mild acute kidney injury. Neutrophil infiltration was similar in ECMO and sham kidneys. Conclusions: Veno-venous extracorporeal circulation deteriorates kidney function and promotes moderate pulmonary damage.

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“…4a). These findings were comparable to our previous data [14], and the lower values were due to hemodilution. Hemoglobin showed a similar drop over the course of the experiments.…”

Section: Hemodynamics and Oxygenation After 4-h Sham And Ecmo Treatmentsupporting

confidence: 92%

Four hours of veno-venous extracorporeal membrane oxygenation using bi-caval cannulation affects kidney function and induces moderate lung damage in a mouse model

Natanov

Khalikov

Gueler

et al. 2019

ICMx

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“…The surgical procedure and CPB were carried out as described previously [ 17 ]. In brief, all animals were anesthetized with isoflurane and orotracheally intubated.…”

Section: Methodsmentioning

confidence: 99%

“…To our knowledge, there is no published literature describing cytokine expression patterns in combination with histological changes after MHCA in a small animal model. In this study, we employed our novel recently-described mouse model of CPB [ 17 ] to assess the cytokine response and its correlation with early histological changes and acute multi-organ injury after MHCA.…”

Section: Introductionmentioning

confidence: 99%

Blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass

et al. 2018

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Cardiopulmonary bypass (CPB) with moderate hypothermic cardiac arrest (MHCA) is essential for prolonged complex procedures in cardiac surgery and is associated with postoperative complications. Although cytokine release provoked through MHCA under CPB plays a pivotal role in postoperative organ damage, the pathomechanisms are unclear. Here, we investigated the cytokine release pattern and histological organ damage after MHCA using a recently described mouse CPB model. Eight BALB/c mice underwent 60 minutes of circulatory arrest under CPB, were successively rewarmed and reperfused. Blood cytokine concentrations and liver and kidney function parameters were measured and histological changes to these organs were compared to control animals. Our results showed a marked increase in proinflammatory cytokines and histological changes in the kidney, lung, and liver after CPB. Furthermore, clinical chemistry showed signs of hemolysis and acute kidney injury. These results suggest early onset of solid organ injury which correlates with increased leukocyte infiltration. A better understanding of the interplay between pro-inflammatory cytokine activation and solid organ injury in this model of CBP with MHCA will inform strategies to reduce organ damage during cardiac surgeries in the clinic.

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“…After intubation, mechanical ventilation was carried out with a small ventilator (respiratory parameters were set as follows: 60 times/min respiratory rate, 2.5 ml/kg tidal volume, and 1: 2 inspiratory-expiratory ratio). The cardiopulmonary bypass was performed as previously described [19]. Catheterization of the caudal vein was used to construct the liquid channel.…”

Section: Animal Modelsmentioning

confidence: 99%

Deficiency of Interleukin-36 Receptor Protected Cardiomyocytes from Ischemia-Reperfusion Injury in Cardiopulmonary Bypass

Luo

Xie

et al. 2020

Med Sci Monit

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Background: Interleukin-36 has been demonstrated to be involved in inflammatory responses. Inflammatory responses due to ischemia-reperfusion injury following cardiopulmonary bypass (CPB) can cause heart dysfunction or damage. Material/Methods: The CPB models were constructed in IL-36R-/-, IL-36RN-/-, and wild-type SD rats. Ultrasonic cardiography and ELISA were used to evaluate the cardiac function and measuring myocardial biomarker levels in different groups. TUNEL assay was used to evaluate apoptosis. Western blot assays and RT-PCR were performed to measure the expression of chemokines and secondary inflammatory cytokines in the heart. Oxidative stress in tissue and cultured cells was assessed using a DCFH-DA fluorescence probe and quantification of superoxide dismutase activity. Results: Improved systolic function and decreased serum levels of myocardial damage biomarkers were found in IL-36R-/-rats compared to WT rats, while worse cardiac function and cardiomyocyte IR injury were observed in IL-36RN-/-rats compared to WT rats. TUNEL staining and Western blot analyses found that cardiomyocyte apoptosis and inflammation were significantly lower in the hearts of IL-36R-/-rats compared with that of WT rats. Oxidative stress was significantly lower in IL-36R-/-rats compared to WT rats. iNOS expression was significantly reduced, while eNOS expression was increased in the hearts of IL-36R-/-rats. Silencing of IL-36R expression in vitro activated SIRT1/FOXO1/p53 signaling in cardiomyocytes. Conclusions: IL-36R deficiency in cardiomyocytes repressed infiltration of bone marrow-derived inflammatory cells and oxidative stress dependent on SIRT1-FOXO1 signaling, thus protecting cardiomyocytes and improving cardiac function in CPB model rats.

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Novel mouse model of cardiopulmonary bypass

Cited by 14 publications

References 13 publications

Four hours of veno-venous extracorporeal membrane oxygenation using bi-caval cannulation affects kidney function and induces moderate lung damage in a mouse model

Four hours of veno-venous extracorporeal membrane oxygenation using bi-caval cannulation affects kidney function and induces moderate lung damage in a mouse model

Blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass

Deficiency of Interleukin-36 Receptor Protected Cardiomyocytes from Ischemia-Reperfusion Injury in Cardiopulmonary Bypass

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