Novel Molecular Recognition via Fluorescent Resonance Energy Transfer Using a Biotin−PEG/Polyamine Stabilized CdS Quantum Dot

Nagasaki, Yukio; Ishii, Takehiko; Sunaga, Yuka; Watanabe, Yousuke; Otsuka, Hidenori; Kataoka, Kazunori

doi:10.1021/la036034c

Cited by 83 publications

(50 citation statements)

References 26 publications

(29 reference statements)

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“…Since the use of ligand-conjugated QDs as fluorescent biolabeling reagents was reported in 1998 by the groups of Alivisatos [51] and Nie [52], many approaches to QD applications have been realized in the bioanalytical field such as DNA sequencing, tissue immune-diagnostics and single molecular imaging [53][54][55][56][57][58][59][60][61][62][63][64][65][66]. The advantages of QDs as biolabeling agents are (i) tunability of the emission wavelength by changing the particle size, (ii) a sharp and symmetrical emission peak, (iii) the high intensity and long lifetime of the fluorescence and (iv) a wide range of excitation wavelengths.…”

Section: Preparation Of Cds Quantum Dots By Co-precipitation In the Pmentioning

confidence: 99%

“…Another problem is the deterioration of the fluorescence characteristics of QDs in aqueous media, which may be due to surface erosion. We recently found that acetal-PEG-b-PAMA can be utilized for both the preparation and stabilization of CdS QDs [57]. Figure 3 shows the results of the co-precipitation of CdCl 2 with Na 2 S with and without various water-soluble polymers.…”

Section: Preparation Of Cds Quantum Dots By Co-precipitation In the Pmentioning

confidence: 99%

“…This is probably due to the immiscibility of the block copolymer segments with each other, viz., PAMA segments are segregated from PEG segments, settling on the QD surface to minimize interfacial free energy, as in the case of the acetal-PEG-b-PAMA-immobilized GNPs described above. Biotin-installed PEGylated CdS QDs also exhibited specific biorecognition, which was monitored via the transfer of fluorescence energy [57].…”

Section: Preparation Of Cds Quantum Dots By Co-precipitation In the Pmentioning

confidence: 99%

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Engineering of poly(ethylene glycol) chain-tethered surfaces to obtain high-performance bionanoparticles

Nagasaki

2010

Science and Technology of Advanced Materials

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A poly(ethylene glycol)-b-poly [2-(N,N-dimethylamino)ethyl methacrylate] block copolymer possessing a reactive acetal group at the end of the poly(ethylene glycol) (PEG) chain, that is, acetal-PEG-b-PAMA, was synthesized by a proprietary polymerization technique. Gold nanoparticles (GNPs) were prepared using the thus-synthesized acetal-PEG-b-PAMA block copolymer. The PEG-b-PAMA not only acted as a reducing agent of aurate ions but also attached to the nanoparticle surface. The GNPs obtained had controlled sizes and narrow size distributions. They also showed high dispersion stability owing to the presence of PEG tethering chains on the surface. The same strategy should also be applicable to the fabrication of semiconductor quantum dots and inorganic porous nanoparticles. The preparation of nanoparticles in situ, i.e. in the presence of acetal-PEG-b-PAMA, gave the most densely packed polymer layer on the nanoparticle surface; this was not observed when coating preformed nanoparticles. PEG/polyamine block copolymer was more functional on the metal surface than PEG/polyamine graft copolymer, as confirmed by angle-dependent x-ray photoelectron spectroscopy. We successfully solubilized the C 60 fullerene into aqueous media using acetal-PEG-b-PAMA. A C 60 /acetal-PEG-b-PAMA complex with a size below 5 nm was obtained by dialysis. The preparation and characterization of these materials are described in this review.

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Section: Preparation Of Cds Quantum Dots By Co-precipitation In the Pmentioning

confidence: 99%

Section: Preparation Of Cds Quantum Dots By Co-precipitation In the Pmentioning

confidence: 99%

Section: Preparation Of Cds Quantum Dots By Co-precipitation In the Pmentioning

confidence: 99%

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Engineering of poly(ethylene glycol) chain-tethered surfaces to obtain high-performance bionanoparticles

Nagasaki

2010

Science and Technology of Advanced Materials

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“…In fact, clever assays for the recognition of various analytes are starting to be designed on the basis of energy transfer processes (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34). Instead, mechanisms based on electron transfer still remain to be explored (35,36).…”

mentioning

confidence: 99%

A mechanism to signal receptor–substrate interactions with luminescent quantum dots

Yildiz

Tomasulo

Raymo

2006

Proc. Natl. Acad. Sci. U.S.A.

135

106

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Semiconductor quantum dots are becoming valuable analytical tools for biomedical applications. Indeed, their unique photophysical properties offer the opportunity to design luminescent probes for imaging and sensing with unprecedented performance. In this context, we have identified operating principles to transduce the supramolecular association of complementary receptor-substrate pairs into an enhancement in the luminescence of sensitive quantum dots. Our mechanism is based on the electrostatic adsorption of cationic quenchers on the surface of anionic quantum dots. The adsorbed quenchers suppress efficiently the emission character of the associated nanoparticles on the basis of photoinduced electron transfer. In the presence of target receptors able to bind the quenchers and prevent electron transfer, however, the luminescence of the quantum dots is restored. Thus, complementary receptor-substrate pairs can be identified with luminescence measurements relying on our design logic. In fact, we have demonstrated with a representative example that our protocol can be adapted to signal protein-ligand interactions.electron transfer ͉ luminescent chemosensors ͉ nanoparticles ͉ proteinligand interactions S emiconductor quantum dots are inorganic nanoparticles with remarkable photophysical properties (1-5). In particular, their one-and two-photon absorption cross-sections, luminescence lifetimes, and photobleaching resistances are significantly greater than those of conventional organic fluorophores. Furthermore, their broad absorption bands extend continuously from the UV to the visible region of the electromagnetic spectrum and, therefore, offer a vast selection of possible excitation wavelengths. Instead, their narrow emission bands can be positioned precisely within the visible and near-infrared regions with fine adjustments of their physical dimensions. In fact, pools of quantum dots with different diameters can be designed to emit in parallel at different wavelengths after excitation at a single wavelength, offering the opportunity to implement unprecedented multichannel assays.Organic dyes do not offer the unique collection of attractive photophysical properties associated with semiconductor quantum dots. Indeed, it is becoming apparent that these inorganic nanoparticles can complement, if not replace, their organic counterparts in a diversity of biomedical applications (6-12). Nonetheless, decades of intensive investigations on the structure and properties of organic chromophores have indicated valuable strategies to design sensitive fluorescent probes able to signal the presence of target analytes with changes in emission intensity (13-16). Their operating principles generally rely on the covalent connection of a fluorescent component to a receptor unit. The receptor is engineered to quench the emission of the fluorophore on the basis of either electron or energy transfer. The supramolecular association of the receptor with a complementary analyte, however, suppresses the quenching mechanism and leads to a si...

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“…For example, CdS quantum dot was prepared by the reaction between CdCl 2 and Na 2 S in the presence of PEG-bpoly(2-(N,N-dimethylamino)ethyl methacrylate) (PEG-b-PAMA) [1]. The size and dispersion stability was controllable in this method.…”

Section: Introductionmentioning

confidence: 99%

Preparation and evaluation of highly dispersible and Ca<sup>2+</sup> level responsive PEGylated calcium phosphate nanoparticle with PEG-<i>block</i>-poly(4-vinylbenzylphosphonate) for the application of drug carrier

Asai

Kanayama

Nagasaki

2012

Trans. Mat. Res. Soc. Japan

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PEGylated calcium phosphate nanoparticles (CaPNPs) were prepared through the simple mixing of calcium and phosphate solutions in the presence of a PEG-block-poly(4-vinylbenzylphosphonate) (PEG-b-PVBP). The PEG-b-PVBP possesses the phosphonate side chains and the hydrophobic polystyrene segment which enhance the stability of the PEG brush layer on the CaPNP surface. The size of CaPNPs was controlled within the range of several tens of nanometer depending on the [PVBP unit]/[phosphate ion] feed-molar ratio upon the CaP coprecipitation reaction. The obtained CaPNPs showed excellent colloidal stability and it was sustained even after the lyophilization. In vitro cell cytotoxicity assay suggested that the CaPNPs do not induce serious cytotoxicity in the murine colon adenocarcinoma 26 (C-26) cells. Light scattering study revealed that the CaPNPs can be dissolved into the low Ca mM). Since intracellular Ca2+ concentration is four orders of magnitude lower than the extracellular one, the Ca 2+ concentration responsive dissolution behavior of CaPNPs is promising for the intracellular delivery of therapeutic materials.

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Novel Molecular Recognition via Fluorescent Resonance Energy Transfer Using a Biotin−PEG/Polyamine Stabilized CdS Quantum Dot

Cited by 83 publications

References 26 publications

Engineering of poly(ethylene glycol) chain-tethered surfaces to obtain high-performance bionanoparticles

Engineering of poly(ethylene glycol) chain-tethered surfaces to obtain high-performance bionanoparticles

A mechanism to signal receptor–substrate interactions with luminescent quantum dots

Preparation and evaluation of highly dispersible and Ca<sup>2+</sup> level responsive PEGylated calcium phosphate nanoparticle with PEG-<i>block</i>-poly(4-vinylbenzylphosphonate) for the application of drug carrier

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