2009
DOI: 10.1021/bi9006836
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Novel Molecular Mechanism for Actinomycin D Activity as an Oncogenic Promoter G-Quadruplex Binder

Abstract: Actinomycin D (ActD) is a natural antibiotic that inhibits the transcription of genes by interacting with a GC-rich duplex, a single-stranded or hairpin form of DNA, and then interfering with the action of RNA polymerase. In this study, we identified a novel molecular mechanism of anticancer activity of ActD as an oncogenic c-Myc promoter G-quadruplex binder. ActD selectively inhibits the elongation of oligonucleotides containing c-Myc promoter G-quadruplex sequence in PCR-stop assays. UV-vis spectroscopic and… Show more

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Cited by 75 publications
(61 citation statements)
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“…Taq DNA polymerase and dNTP mixtures were purchased from Koma Biotech (Korea). The principles and procedures for this experiment were previously described [37]. PCR was performed in a reaction buffer containing 10 mM Tris-HCl (pH 7.6), 50 mM KCl and 1.5 mM Mg (OAc) 2 with 0.2 lM of each oligonucleotide.…”
Section: Pcr-stop Assay/native Page (Polyacrylamide Gel Electrophoresis)mentioning
confidence: 99%
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“…Taq DNA polymerase and dNTP mixtures were purchased from Koma Biotech (Korea). The principles and procedures for this experiment were previously described [37]. PCR was performed in a reaction buffer containing 10 mM Tris-HCl (pH 7.6), 50 mM KCl and 1.5 mM Mg (OAc) 2 with 0.2 lM of each oligonucleotide.…”
Section: Pcr-stop Assay/native Page (Polyacrylamide Gel Electrophoresis)mentioning
confidence: 99%
“…The method for real-time PCR was previously described [37]. Two B-cell lymphoma cell lines, Ramos and CA46, were treated with ligands for 48 h and total RNA was extracted by Easy-Blue RNA extraction kits (Intron Biotechnology).…”
Section: Real-time Pcrmentioning
confidence: 99%
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“…세포 독성과 항종양성을 가진다고 알려져 있으며 암세포의 DNA에 작용하 는 항암제로 알킬화제에 포함된다 [12,13]. Actinomycin D는 세포자멸사(apoptosis)를 일으키는 강력한 유도물질로 DNA 에 결합하여 유전 정보 전사를 저해한다 [8,13]. 이들 유사 약물 은 생식세포종양(germ cell tumors), 연조직육종(soft-tissue sarcoma), 흑색종(melanoma), 융모암종(choriocarcinoma) 등 의 치료에 사용되는 항암 화학 치료 약물이다.…”
Section: 서 론unclassified