Novel modeling methodology for the characterization of enzymatic hydrolysis of proteins

Valencia, Pedro; Espinoza, Karen; Ceballos, Andrés; Pinto, Marlene; Almonacid, Sergio

doi:10.1016/j.procbio.2014.12.028

Cited by 31 publications

(21 citation statements)

References 29 publications

(71 reference statements)

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“…However, because of the different pretreatment to substrates, leading to the undefined nature of substrates, thus enzymatic hydrolysis is a highly complex process in the heterogeneous reaction system and so on. [17] However, there is scare literature that focuses on the enzymolysis kinetics and the functional and structural characteristics of HIU-assisted alkali pretreatment OVA. Therefore, in this study, we used HIU-assisted alkali to treat with OVA before hydrolyzing by Alcalase, with the aim to further explore the structural changes of OVA, the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), fluorescence spectrometry, surface hydrophobicity, and Fourier transform infrared (FT-IR) were performed.…”

Section: Introductionmentioning

confidence: 99%

Enzymolysis kinetics and structural-functional properties of high-intensity ultrasound-assisted alkali pretreatment ovalbumin

Liu

Hao

Dai

et al. 2020

International Journal of Food Properties

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This research investigated the enzymolysis kinetics of high-intensity ultrasound (HIU)-assisted alkali pretreatment Ovalbumin (OVA) through using Alcalase, and its structural and functional properties. The structural characteristics of OVA were performed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), surface hydrophobicity, Fourier Transform Infrared, and differential scanning calorimetry. SDS-PAGE analysis indicated that the pretreatment sample showed proportion of lower molecular mass components (14.3-20.1 kDa). There was an increase in the intensity of fluorescence spectrum and changes in functional groups after the pretreatment on OVA compared with the control (without treatment), which might be the reason for the significantly enhancing efficiency of the enzymatic hydrolysis. The thermal denaturation temperature of enzymatic hydrolysis of OVA after pretreatment was 95.1°C, which was 17.5°C higher than that of control. As compared to OVA (control), the enzymatic hydrolysis with HIU-assisted alkali pretreatment was found to significantly improve protein solubility, emulsifying properties, foaming ability (FA) and foam stability, oil, and water absorption capacity (WAC). Especially emulsifying activity index, FA, WAC, and oil absorption capacity were increased by 228.7%, 88.5%, 102.6%, and 67.4%, respectively (P< 0.05). A simplified kinetic equation for the enzymolysis model was deduced to successfully describe the enzymatic hydrolysis of pretreated OVA. The results proved the effects of the pretreatment on the substrate enhanced the enzymolysis process and this influence had relation to the structure changes. Furthermore, the enzymatic hydrolysis process for other proteins demonstrated the applicability of the model.

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Section: Introductionmentioning

confidence: 99%

Enzymolysis kinetics and structural-functional properties of high-intensity ultrasound-assisted alkali pretreatment ovalbumin

Liu

Hao

Dai

et al. 2020

International Journal of Food Properties

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“…These parameters are normalized to compare the effects and the influence of the studied function. A rotatable central composite design was used and consists of three distinct sets of experimental runs: a set of central points, a full factorial design (orthogonal) and a set of axial points identical to the central point except for one parameter (values outside the studied range) [28,31]. All the experimental tests are systematically repeated 3 times.…”

Section: Methodsmentioning

confidence: 99%

Prediction of the mechanical properties of zeolite pellets for aerospace molecular decontamination applications

Rioland¹,

Dutournié²,

Faye³

et al. 2016

Beilstein J. Nanotechnol.

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Zeolite pellets containing 5 wt % of binder (methylcellulose or sodium metasilicate) were formed with a hydraulic press. This paper describes a mathematical model to predict the mechanical properties (uniaxial and diametric compression) of these pellets for arbitrary dimensions (height and diameter) using a design of experiments (DOE) methodology. A second-degree polynomial equation including interactions was used to approximate the experimental results. This leads to an empirical model for the estimation of the mechanical properties of zeolite pellets with 5 wt % of binder. The model was verified by additional experimental tests including pellets of different dimensions created with different applied pressures. The optimum dimensions were found to be a diameter of 10–23 mm, a height of 1–3.5 mm and an applied pressure higher than 200 MPa. These pellets are promising for technological uses in molecular decontamination for aerospace-based applications.

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“…This tool used in many fields [40][41][42] allows obtaining the maximum of information about the operating parameters influence, from a minimum of tests. In this case, the influence of three reduction parameters was studied (temperature, mass flow rate and ratio between inlet concentration of CO and amount of oxygen available on the oxygen carrier).…”

Section: Experiments In Fixed Bed Reactormentioning

confidence: 99%

“…The integration of the breakthrough curves (oxidized CO or produced CO 2 ) given in Table 1, column 6 corroborates previous observations. Figure 2 shows the breakthrough curves of CO obtained during experimental tests (tests 14, 16 and 20) performed at different flow rates (20,40 and 60 NL/h). As expected the higher the flow rate is, the faster the percolation of CO is.…”

Section: Experiments In Fixed Bed Reactormentioning

confidence: 99%

Numerical Modeling of Oxygen Carrier Performances (NiO/NiAl2O4) for Chemical-Looping Combustion

Blas¹,

Dutournié

Jeguirim

et al. 2017

Energies

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Abstract:This work was devoted to study experimentally and numerically the oxygen carrier (NiO/NiAl 2 O 4 ) performances for Chemical-Looping Combustion applications. Various kinetic models including Shrinking Core, Nucleation Growth and Modified Volumetric models were investigated in a one-dimensional approach to simulate the reactive mass transfer in a fixed bed reactor. The preliminary numerical results indicated that these models are unable to fit well the fuel breakthrough curves. Therefore, the oxygen carrier was characterized after several operations using Scanning Electronic Microscopy (SEM) coupled with equipped with an energy dispersive X-ray spectrometer (EDX). These analyses showed a layer rich in nickel on particle surface. Below this layer, to a depth of about 10 µm, the material was low in nickel, being the consequence of nickel migration. From these observations, two reactive sites were proposed relative to the layer rich in nickel (particle surface) and the bulk material, respectively. Then, a numerical model, taking into account of both reactive sites, was able to fit well fuel breakthrough curves for all the studied operating conditions. The extracted kinetic parameters showed that the fuel oxidation was fully controlled by the reaction and the effect of temperature was not significant in the tested operating conditions range.

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Novel modeling methodology for the characterization of enzymatic hydrolysis of proteins

Cited by 31 publications

References 29 publications

Enzymolysis kinetics and structural-functional properties of high-intensity ultrasound-assisted alkali pretreatment ovalbumin

Enzymolysis kinetics and structural-functional properties of high-intensity ultrasound-assisted alkali pretreatment ovalbumin

Prediction of the mechanical properties of zeolite pellets for aerospace molecular decontamination applications

Numerical Modeling of Oxygen Carrier Performances (NiO/NiAl2O4) for Chemical-Looping Combustion

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