Novel microtubule-associated protein-2 isoform is expressed early in human oligodendrocyte maturation

Abstract

We have identified a developmentally regulated, oligodendrocyte-specific protein, designated microtubule-associated protein-2 expressing exon 13 (MAP-2+13), in the human central nervous system (CNS). Monoclonal antibodies directed against MAP-2+13 labeled oligodendrocytes in the white matter of human fetal spinal cord. Double-label immunofluorescence and confocal microscopy, and immunoelectron microscopy localized MAP-2+13 to the soma and extending processes of fetal oligodendrocytes, but not to the myelin sheath. The immunoreactivity was throughout the perikarya. Ultrastructural examination of the fetal myelin sheaths showed them to be thin and not fully compacted, indicating that myelination was in progress. There was no overlap in staining of GFAP+ astrocytes and MAP-2+13+ oligodendrocytes. MAP-2+13 was also expressed in intermediate filament-negative "radial glia" extending from the central canal to the subpial surface. In the mature CNS, MAP-2+13 also marked cells of oligodendroglial morphology, but these cells were rare. These finding demonstrate that in the human CNS, MAP-2+13 is a novel protein transiently expressed in cells of oligodendroglial lineage.

A tumor-specific cellular environment at the brain invasion border of adamantinomatous craniopharyngiomas

“…To our knowledge, the only exceptions are MAP2c-expressing oligodendroglial progenitors occurring in multiple sclerosis plaques [48] or in HIV encephalitis [49]. In contrast to adult CNS in which MAP2a, MAP2b, and MAP2c are confined to neurons, glial precursor cells transiently express an alternatively spliced embryonic transcript including exon 13 (MAP2e) [33,36]. We uncovered a peculiar cell population offering a glial phenotype with a distinct expression of both the embryonic and the adult transcript of MAP2.…”

“…It binds to and stabilizes microtubules and is involved in cell proliferation as well as neuronal differentiation [28][29][30]. Each MAP2 transcript is the result of alternative splicing of a single gene located on human chromosome 2 [31][32][33]. The cellular expression of MAP2 and its splice forms are precisely regulated during brain development as well as in mature nervous tissue [28].…”

You-Gui pills promote nerve regeneration by regulating netrin1, DCC and Rho family GTPases RhoA, Racl, Cdc42 in C57BL/6 mice with experimental autoimmune encephalomyelitis

“…Studies have shown that MAP-2 expression was significantly increased during nerve injury, which represented reconstruction of dendritic structure to improve neurological function (Lin et al, 2015;Shafit-Zagardo et al, 2000;Uchida et al, 2015). MAP-2 is an important structural and functional protein found in the neuron bodies and dendrites for maintaining microtubule stability and nerve regeneration (Mohan and John, 2015).…”

Antisense suppression of tau in cultured rat oligodendrocytes inhibits process formation

“…Oligodendrocytes express several proteins in addition to tau that can contribute to oligodendrocyte microtubule assembly, stabilization, and organization during development. MAP1B and isoforms of MAP2 (Vouyiouklis and Brophy, 1993, 1995; Shafit‐Zagardo et al, 2000) are developmentally regulated in oligodendrocytes. Oligodendrocyte‐specific STOP (stable tubule‐only polypeptide) isoforms (Galiano et al, 2004) have been described, and microtubule interactions have been suggested for the myelin proteins 2′,3′‐cyclic nucleotide‐3′‐phosphodiesterase (CNP; Bifulco et al, 2002; Lee et al, 2005) and MBP (Galiano et al, 2006) as well as for qk1 (Zhao et al, 2006), stathmins (Liu et al, 2003; Southwood et al, 2004, 2007), and Fyn (Klein et al, 2002).…”