Novel microtubule-associated protein-2 isoform is expressed early in human oligodendrocyte maturation

Shafit‐Zagardo, Bridget; Davies, Peter; Rockwood, Julia M.; Kress, Yvonne; Lee, Sunhee C.

doi:10.1002/(sici)1098-1136(20000201)29:3<233::aid-glia5>3.0.co;2-u

Cited by 28 publications

(14 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, Neu-N may be a useful immunostain for excluding the native ganglion cells that occasionally show mildly dysmorphic features in the midst of an infiltrating glioma. It is possible that MAP2 staining patterns could further aid in this distinction [5, 7, 32, 34], although this marker was not generally available in the primary author’s institution and was also not utilized by any of the referring pathologists. Therefore, future studies are needed to assess its potential utility for this differential diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Oligodendroglial neoplasms with ganglioglioma-like maturation: a diagnostic pitfall

et al. 2010

View full text Add to dashboard Cite

Although oligodendroglial neoplasms are traditionally considered purely glial, increasing evidence suggests that they are capable of neuronal or neurocytic differentiation. Nevertheless, ganglioglioma-like foci (GGLF) have not been previously described. Herein, we report seven examples where the primary differential diagnosis was a ganglioglioma with an oligodendroglial component. These five male and two female patients ranged in age from 29 to 63 (median 44) years at initial presentation and neuroimaging features were those of diffuse gliomas in general. At presentation, the glial component was oligodendroglioma in six and oligoastrocytoma in one; one was low-grade and six were anaplastic. A sharp demarcation from adjacent GGLF was common, although some intermingling was always present. The GGLF included enlarged dysmorphic and occasionally binucleate ganglion cells, Nissl substance, expression of neuronal antigens, GFAP-positive astrocytic elements, and low Ki-67 labeling indices. In contrast to classic ganglioglioma, however, cases lacked eosinophilic granular bodies and CD34-positive tumor cells. Scattered bizarre astrocytes were also common and one case had focal neurocytic differentiation. By FISH analysis, five cases showed 1p/19q codeletion. In the four cases with deletions and ample dysmorphic ganglion cells for analysis, the deletions were found in both components. At last follow-up, two patients suffered recurrences, one developed radiation necrosis mimicking recurrence, and one died of disease 7.5 years after initial surgery. We conclude that GGLF represents yet another form of neuronal differentiation in oligodendroglial neoplasms. Recognition of this pattern will prevent a misdiagnosis of ganglioglioma with its potential for under-treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Oligodendroglial neoplasms with ganglioglioma-like maturation: a diagnostic pitfall

et al. 2010

View full text Add to dashboard Cite

show abstract

“…4; Table 3). The MAP2 epitope of interest is an alternatively spliced transient (embryonic) isoform including exon 13 (MAP2E) [6, 82]. This isoform is likely to occur also in astroglial precursors [6] and can be detected in the vast majority of diffusely infiltrating gliomas [10, 76].…”

Section: Lack Of Diagnostic Agreement Requires New Concepts: Proposalmentioning

confidence: 99%

A neuropathology-based approach to epilepsy surgery in brain tumors and proposal for a new terminology use for long-term epilepsy-associated brain tumors

et al. 2014

View full text Add to dashboard Cite

Every fourth patient submitted to epilepsy surgery suffers from a brain tumor. Microscopically, these neoplasms present with a wide-ranging spectrum of glial or glio-neuronal tumor subtypes. Gangliogliomas (GG) and dysembryoplastic neuroepithelial tumors (DNTs) are the most frequently recognized entities accounting for 65 % of 1,551 tumors collected at the European Epilepsy Brain Bank (n = 5,842 epilepsy surgery samples). These tumors often present with early seizure onset at a mean age of 16.5 years, with 77 % of neoplasms affecting the temporal lobe. Relapse and malignant progression are rare events in this particular group of brain tumors. Surgical resection should be regarded, therefore, also as important treatment strategy to prevent epilepsy progression as well as seizure- and medication-related comorbidities. The characteristic clinical presentation and broad histopathological spectrum of these highly epileptogenic brain tumors will herein be classified as “long-term epilepsy associated tumors—LEATs”. LEATs differ from most other brain tumors by early onset of spontaneous seizures, and conceptually are regarded as developmental tumors to explain their pleomorphic microscopic appearance and frequent association with Focal Cortical Dysplasia Type IIIb. However, the broad neuropathologic spectrum and lack of reliable histopathological signatures make these tumors difficult to classify using the WHO system of brain tumors. As another consequence from poor agreement in published LEAT series, molecular diagnostic data remain ambiguous. Availability of surgical tissue specimens from patients which have been well characterized during their presurgical evaluation should open the possibility to systematically address the origin and epileptogenicity of LEATs, and will be further discussed herein. As a conclusion, the authors propose a novel A–B–C terminology of epileptogenic brain tumors (“epileptomas”) which hopefully promote the discussion between neuropathologists, neurooncologists and epileptologists. It must be our future mission to achieve international consensus for the clinico-pathological classification of LEATs that would also involve World Health Organization (WHO) and the International League against Epilepsy (ILAE).

show abstract

“…Cortical GAP-43 thus appears earlier than synaptic vesicles are demonstrated by synaptophysin immunoreactivity or by electron microscopy. It is strongly expressed in regenerating optic nerve in fish [141, 142], which may correlate with its high expression in growing fetal axones of the cortex and white matter. In kainic acid-induced seizures in rodents, GAP-43 also is seen in the cortex [143].…”

Section: Immunocytochemical Markersmentioning

confidence: 99%

Clinical neuropathology practice guide 5-2013: markers of neuronal maturation

Sarnat¹

2013

View full text Add to dashboard Cite

This review surveys immunocytochemical and histochemical markers of neuronal lineage for application to tissue sections of fetal and neonatal brain. They determine maturation of individual nerve cells as the tissue progresses to mature architecture. From a developmental perspective, neuronal markers are all about timing. These diverse cellular labels may be classified in two ways: 1) time of onset of expression (early; intermediate; late); 2) labeling of subcellular structures or metabolic functions (nucleoproteins; synaptic vesicle proteins; enolases; cytoskeletal elements; calcium-binding; nucleic acids; mitochondria). Apart from these positive markers of maturation, other negative markers are expressed in primitive neuroepithelial cells and early stages of neuroblast maturation, but no longer are demonstrated after initial stages of maturation. These examinations are relevant for studies of normal neuroembryology at the cellular level. In fetal and perinatal neuropathology they provide control criteria for application to malformations of the brain, inborn metabolic disorders and acquired fetal insults in which neuroblastic maturation may be altered. Disorders, in which cells differentiate abnormally, as in tuberous sclerosis and hemimegalencephaly, pose another yet aspect of mixed cellular lineage. The measurement in living patients, especially neonates, of serum and CSF levels of enolases, chromogranins and S-100 proteins as biomarkers of brain damage may potentially be correlated with their corresponding tissue markers at autopsy in infants who do not survive. The neuropathological markers here described can be performed in ordinary hospital laboratories, not just research facilities, and offer another dimension of diagnostic precision in interpreting abnormally developed fetal and postnatal brains.

show abstract

Novel microtubule-associated protein-2 isoform is expressed early in human oligodendrocyte maturation

Cited by 28 publications

References 50 publications

Oligodendroglial neoplasms with ganglioglioma-like maturation: a diagnostic pitfall

Oligodendroglial neoplasms with ganglioglioma-like maturation: a diagnostic pitfall

A neuropathology-based approach to epilepsy surgery in brain tumors and proposal for a new terminology use for long-term epilepsy-associated brain tumors

Clinical neuropathology practice guide 5-2013: markers of neuronal maturation

Contact Info

Product

Resources

About