Novel Microfluidics Device for Rapid Antibiotics Susceptibility Screening

Abstract

In recent years, excessive utilization of antibiotics has led to the emergence of antibiotic microbial resistance on a planetary scale. This recent phenomenon represents a serious threat to public health, as well as an enormous burden for healthcare systems’ budgets worldwide. Novel, rapid and cheap methods for antibiotic susceptibility screening are urgently needed for this obstacle to be overcome. In this paper, we present a microfluidic device for on-chip antibiotic resistance testing, which allows for antibiotic microbial resistance detection within 6 hours. The design, fabrication and experimental utilization of the device are thoroughly described and analyzed, as well as possibilities for future automation of the whole process. The accessibility of such a device for all people, regardless of economic status, was of utmost importance for us during the development of the project.

Low-Cost Three-Dimensionally Printed Inverted Plug and Play Optical Instrument for Microfluidic Imaging

“…All devices were created from polydimethylsiloxane (PDMS) and poured into a three-dimensionally printed resin mold. The procedure was already described in our previous work [16]. In the first experiment, a flow-focusing junction for droplet generation was created, similar to the one described in [17].…”

“…Bacteria cell growth was then monitored visually. A detailed description of the results was carried out in [16]. Figure 6 shows a picture made with the above-described inversed microscope of the novel chip 1 h after the filling of the chambers and cultivation channels.…”

“…A bacterial growth is detected in the channel without antibiotics (lower pixel intensity). With the help of a microscope, one can easily detect antibiotic resistance after approximately 6 h [16], without the need for single-cell analysis. Even though the results of other studies regarding antibiotic Micro 2023, 3 544 susceptibility screening look more promising, the proposed microscope offers an affordable tool for point-of-care diagnostics.…”

“…Dimensions of the shown microchip section are 11 (L) × 3.5 (W) mm. A detailed description of the device can be found in [16]. A detailed description of the device can be found in [16].…”

Electromicrofluidic Device for Interference-Free Rapid Antibiotic Susceptibility Testing of Escherichia coli from Real Samples

“…However, the developed device is complex and requires more attention to the environmental effect on fluid flow inside the channel [22]. Hence, microfluidic integrated with electrochemical sensing further improves the detection efficacy and increases the simplicity and sensitivity of detection [23,24].…”