Novel methods for liposome preparation

Patil, Yogita P.; Jadhav, Sameer

doi:10.1016/j.chemphyslip.2013.10.011

Cited by 481 publications

(270 citation statements)

References 144 publications

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“…On the other hand, to increase liposomes stability their surface can be decorated with various polymers (Meland et al 2014). Several review articles have been published (Akbarzadeh et al 2013;Jones 1995;Barenholz 2001;Patil and Jadhav 2014) on preparation, characterization and applications of liposomes where above mentioned aspects are discussed.…”

Section: Introductionmentioning

confidence: 99%

Zeta potential and surface charge of DPPC and DOPC liposomes in the presence of PLC enzyme

2016

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Zeta potential of liposomes is often measured in studies of their properties and/or applications. However, there are not many papers published in which zeta potential was determined during enzymatic reaction of a lipid. Therefore in this paper size, polydispersity index, and zeta potentials of 1,2-dipalmitoylsn-glycero-3-phosphatidylcholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) liposomes were investigated in 1 mM NaCl (pH 6.2) and phosphate buffer (pH 8.1) during 60 min of phospholipase C action at 20 and 37°C. The hydrocarbon chains saturation differ these two phospholipids what appears in some differences in the zeta potential changes during the hydrolysis reaction run. The polydispersity index of the liposomes indicates that they are relatively stable and monodisperse, except for DPPC in phosphate buffer where up to 30 % of the initial amount forms larger size moieties, possibly aggregates of the liposomes, enzyme and the hydrolysis products. However, in this buffer zeta potential of the liposomes practically does not change during PLC action. Also the changes of zeta potential of DOPC liposomes are minor, although their negative values are much smaller than those of DPPC at both temperatures. These small changes of the potential may be due to compression of the diffuse double layer by present phosphate buffer. However, distinct changes of the zeta potentials in the presence of PLC take place in NaCl solution. The observed changes can be explained by reorientation of the phospholipid polar heads and their different density on DPPC and DOPC surface of the liposomes. Although it appeared that the zeta potential is not a very sensitive parameter for tracking the hydrolysis reaction in phosphate buffer, generally zeta potential enables the characterization of such reactions through determination of electrokinetic properties of liposomes as well as the polydispersity and size distribution of the liposomes do.

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Section: Introductionmentioning

confidence: 99%

Zeta potential and surface charge of DPPC and DOPC liposomes in the presence of PLC enzyme

2016

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“…Small unilamellar vesicles (SUVs) are composed of only one phospholipid bilayer and a small aqueous compartment with a diameter ranging from 20 to 100 nm (Yeagle 1993;Akbarzadeh et al 2013). Among the various methods reported in the literature for liposome preparation, the most frequently used are lipid hydration, ethanol injection, freeze-thawing and reverse phase evaporation (Akbarzadeh et al 2013;Patil and Jadhav 2014).…”

Section: Liposomesmentioning

confidence: 99%

“…Residual organic solvents may become toxic and degrade active ingredients, thus representing a possible risk to human health (Birnbaum et al 2000). In the case of vesicles carrying proteins, organic solvents or detergents can denaturate the proteins and affect the membrane properties (Seddon et al 2004), thus influencing the stability of vesicles (Wagner et al 2002;Wagner and Vorauer-Uhl 2011;Patil and Jadhav 2014).…”

Section: Liposomesmentioning

confidence: 99%

“…However, their cell-like structure led to investigations on their efficacy as a tool to carry potent molecules into the human body. Nowadays they are used for the encapsulation of unstable compounds considered to be essential to human health, such as antioxidants, antimicrobials, bioactive elements, antigenic proteins, thereby preserving their functionality (Madni et al 2014;Schwendener 2014;Patil and Jadhav 2014). Liposomal vesicles have emerged as one of the novel carrier systems most studied by the scientific community over the past decades and have revolutionized research in many different fields, such as cosmetics, drugs, food, agriculture, with applications in chemotherapy treatments, gene therapy, vaccines, antimicrobial agents, among others (Madni et al 2014).…”

Section: Liposomal Systems As Carriers For Bioactive Compoundsmentioning

confidence: 99%

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Liposomal systems as carriers for bioactive compounds

et al. 2015

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Since the revolutionary discovery that phospholipids can form closed bilayered structures in aqueous systems, the study of liposomes has become a very interesting area of research. The versatility and amazing biocompatibility of liposomes has resulted in their wide-spread use in many scientific fields, and many of their applications, especially in medicine, have yielded breakthroughs in recent decades. Specifically, their easy preparation and various structural aspects have given rise to broadly usable methodologies to internalize different compounds, with either lipophilic or hydrophilic properties. The study of compounds with potential biotechnological application(s) is generally related to evaluation and risk assessment of the possible cytotoxic or therapeutic effects of the compound under study. In most cases, undesirable sideeffects are associated with an interaction of the liposome with the cell membrane and/or its absorption and subsequent interaction with a cellular biomolecule. Liposomal carrier systems have an unprecedented potential for delivering bioactive substances to specific molecular targets due to their biocompatibility, biodegradability and low toxicity. Liposomes are therefore considered to be an invaluable asset in applied biotechnology studies due to their potential for interaction with both hydrophilic and lipophilic compounds.

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“…There are a variety of methods available to produce liposomes [1]. The preparation methods have been classified depending on mean size, polydispersity and lamellarity of liposomes obtained as these are challenging factors with almost all preparation methods [96]. Considering the size and encapsulation of vaterite particles, we tried four different methods as described below.…”

Section: Liposome Formationmentioning

confidence: 99%

High Resolution Measurements of Viscoelastic Properties of Complex Biological Systems Using Rotating Optical Tweezers

Zhang¹

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The aim of this thesis is to investigate the viscoelastic properties of systems on the microscale at extremely low sample volumes and in highly limited spaces with high temporal resolution. A method based on both passive and active rotational tweezers is presented, which enables measurements of viscoelasticity with high resolution and within a broadband frequency range.Cellular mechanics and rheological properties play an important role in biological processes (e.g., transport of intracellular organelles by molecular motors, cellular processes, endocytic trafficking, axonal retrograde transport in neurons and secretory pathways). All these activities occur in cytoplasm which behaves both as an elastic solid and as a viscous fluid, and is considered viscoelastic. Therefore, the quantitative characterization of viscoelasticity in cells can help us to understand cellular mechanisms.This thesis begins by introducing optical tweezers elucidating transfer of both linear and angular momentum of light and describing methods to apply and measure optical torques on birefringent particles. Microspherical vaterite particles are synthesized for our experiments using a method developed and refined in our lab. These particles are composed of the calcium carbonate mineral vaterite and are birefringent. Next I describe both theoretical and experimental methods of determining viscoelasticity by measuring the angular diffusion of displacement of a trapped vaterite

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Novel methods for liposome preparation

Cited by 481 publications

References 144 publications

Zeta potential and surface charge of DPPC and DOPC liposomes in the presence of PLC enzyme

Zeta potential and surface charge of DPPC and DOPC liposomes in the presence of PLC enzyme

Liposomal systems as carriers for bioactive compounds

High Resolution Measurements of Viscoelastic Properties of Complex Biological Systems Using Rotating Optical Tweezers

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