Novel method of intraoperative liver tumour localisation with indocyanine green and near-infrared imaging

Lim, Hui Jun; Chiow, Adrian Kah Heng; Lee, Lip Seng; Tan, Siong San; Goh, Brian K. P.; Koh, Ye Xin; Chan, Chung Yip; Lee, Ser Yee

doi:10.11622/smedj.2019137

Cited by 13 publications

(14 citation statements)

References 35 publications

(55 reference statements)

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“…However, the flexibility of medical resource allocation might be poorer and the waiting time before surgery would be prolonged in this setting. A small retrospective study indicated that a shorter injection time can also result in a satisfactory imaging effect [ 16 ]. However, as the ICG administration timing remains inconclusive, another cohort study was begun last year [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Despite all these advantages, ICG fluorescence navigation has some inherent drawbacks. First, ICG is usually injected intravenously a few days prior to the operation and bile metabolism malfunctions in diseased (especially cirrhotic) liver, and residual ICG will affect the observation [ 13 , 16 ]. Second, regenerated nodules might show as false-positive if the bile duct is compressed [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Indocyanine green fluorescence navigation in laparoscopic hepatectomy: a retrospective single-center study of 120 cases

Liang

Qian

et al. 2020

Surg Today

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Purpose To explore the role of indocyanine green (ICG) fluorescence navigation in laparoscopic hepatectomy and investigate if the timing of its administration influences the intraoperative observation. Methods The subjects of this retrospective study were 120 patients who underwent laparoscopic hepatectomy; divided into an ICG-FN group (n = 57) and a non-ICG-FN group (n = 63). We analyzed the baseline data and operative data. Results There were no remarkable differences in baseline data such as demographic characteristics, lesion-related characteristics, and liver function parameters between the groups. Operative time and intraoperative blood loss were significantly lower in the ICG-FN group. The rate of R0 resection of malignant tumors was comparable in the ICG-FN and non-ICG-FN groups, but the wide surgical margin rate was significantly higher in the ICG-FN group. The administration of ICG 0–3 or 4–7 days preoperatively did not affect the intraoperative fluorescence imaging. Operative time, intraoperative blood loss, and a wide surgical margin correlated with ICG fluorescence navigation. ICG fluorescence navigation helped to minimize intraoperative blood loss and achieve a wide surgical margin. Conclusion ICG fluorescence navigation is safe and efficient in laparoscopic hepatectomy. It helps to achieve a wide surgical margin, which could result in a better prognosis. The administration of ICG 0–3 days preoperatively is acceptable.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Indocyanine green fluorescence navigation in laparoscopic hepatectomy: a retrospective single-center study of 120 cases

Liang

Qian

et al. 2020

Surg Today

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“…Based on sequence information, mouse OATP1B2 is orthologous to human OATP1B1 and OATP1B3 (approximately 70% shared identity), whereas mouse OATP1A1 is orthologous to human OATP1A2, which is expressed in the brain [20]. A subsequent study indicated that the uptake deficit of liver-specific MRI contrast agents in Slco1a/1b knockout mice could be rescued by knock-in of human OATP1B1/1B3 [17]. The different enhancement levels between Gd-EOB-DTPA and Gd-BOPTA in OATP1B1/1B3 knock-in mice may be due to the expression ratio of OATP1B1 and OATP1B3, as evidenced in our observation that OATP1B1 is a better transporter for Gd-BOPTA than OATP1B3 is.…”

Section: Discussionmentioning

confidence: 99%

“…Previous inhibition studies and activity modification via the protein kinase C pathway showed that Gd-BOPTA is transported through OATPs and that its efflux from hepatocytes is via multiple resistanceassociated protein 3 [15,16]. However, other results identified human OATP1B1 and OATP1B3 expression in Slco1a/1b knockout mice and that OATP1B1/1B3 could not fully rescue the transportation of Gd-BOPTA in knockout mice [17]. According to these studies, the OATP family transportability toward Gd-BOPTA is not determined and we intend to use a cell line transduction model to investigate the Gd-BOPTA transport capabilities of human OATP1B1 and OATP1B3 separately.…”

Section: Introductionmentioning

confidence: 95%

Organic Anion Transporting Polypeptide 1B1 Is a Potential Reporter for Dual MR and Optical Imaging

Lee

Hsiao

2021

IJMS

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Membrane proteins responsible for transporting magnetic resonance (MR) and fluorescent contrast agents are of particular importance because they are potential reporter proteins in noninvasive molecular imaging. Gadobenate dimeglumine (Gd-BOPTA), a liver-specific MR contrast agent, has been used globally for more than 10 years. However, the corresponding molecular transportation mechanism has not been validated. We previously reported that the organic anion transporting polypeptide (OATP) 1B3 has an uptake capability for both MR agents (Gd-EOB-DTPA) and indocyanine green (ICG), a clinically available near-infrared (NIR) fluorescent dye. This study further evaluated OATP1B1, another polypeptide of the OATP family, to determine its reporter capability. In the OATP1B1 transfected 293T transient expression model, both Gd-BOPTA and Gd-EOB-DTPA uptake were confirmed through 1.5 T MR imaging. In the constant OAPT1B1 and OATP1B3 expression model in the HT-1080 cell line, both HT-1080-OAPT1B1 and HT-1080-OATP1B3 were observed to ingest Gd-BOPTA and Gd-EOB-DTPA. Lastly, we validated the ICG uptake capability of both OATP1B1 and OATP1B3. OAPT1B3 exhibited a superior ICG uptake capability to that of OAPT1B1. We conclude that OATP1B1 is a potential reporter for dual MR and NIR fluorescent molecular imaging, especially in conjunction with Gd-BOPTA.

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“…and the lack of standardised and validated quantitative models. In this issue of the Singapore Medical Journal, Lim et al (15) described their experience with NIR imaging using ICG for intraoperative liver tumour localisation in 32 patients. A total of 46 lesions were resected, including 23 hepatocellular carcinomas, 12 colorectal liver metastases and 11 benign lesions.…”

mentioning

confidence: 99%

Fluorescence imaging in hepatobiliary surgery – hope and hype

Iyer¹

2021

smedj

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Novel method of intraoperative liver tumour localisation with indocyanine green and near-infrared imaging

Cited by 13 publications

References 35 publications

Indocyanine green fluorescence navigation in laparoscopic hepatectomy: a retrospective single-center study of 120 cases

Indocyanine green fluorescence navigation in laparoscopic hepatectomy: a retrospective single-center study of 120 cases

Organic Anion Transporting Polypeptide 1B1 Is a Potential Reporter for Dual MR and Optical Imaging

Fluorescence imaging in hepatobiliary surgery – hope and hype

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