Novel Method for the Preparation of Controlled-Release Theophylline Granules Coated with a Polyelectrolyte Complex of Sodium Polyphosphate-Chitosan

Kawashima, Yasunaru; Handa, Tetsurou; Kasai, Atsushi; Takenaka, Hideo; Lin, Shan‐Yang; Ando, Yutaka

doi:10.1002/jps.2600740308

Cited by 153 publications

(66 citation statements)

References 9 publications

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“…[23][24][25] Studies have shown that chitosan is a promising DNA vector with sustained-releasing ability. 26 Chitosan/DNA particle system prepared by this ionic gelation technique is suitable for mammalian cell transfection, and the expression level of the reporter gene with the chitosan particle system was comparable to that produced by the positive control. 27 Therefore, chitosan nanoparticles as a novel and efficient gene transduction vector, by use of their targeted and sustained release, 28,29 can greatly enhance transfection and expression efficiency of DNA vaccines, thereby increasing their bioavailability.…”

Section: Introductionmentioning

confidence: 87%

Preparation and efficacy of Newcastle disease virus DNA vaccine encapsulated in chitosan nanoparticles

Zhao¹,

Zhang²,

Zhang³

et al. 2014

IJN

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Optimal preparation conditions of Newcastle disease virus (NDV) F gene deoxyribonucleic acid (DNA) vaccine encapsulated in chitosan nanoparticles (pFNDV-CS-NPs) were determined. The pFNDV-CS-NPs were prepared according to a complex coacervation method. The pFNDV-CS-NPs were produced with good morphology, high stability, a mean diameter of 199.5 nm, encapsulation efficiency of 98.37%±0.87%, loading capacity of 36.12%±0.19%, and a zeta potential of +12.11 mV. The in vitro release assay showed that the plasmid DNA was sustainably released from the pFNDV-CS-NPs, up to 82.9%±2.9% of the total amount. Cell transfection test indicated that the vaccine expressed the F gene in cells and maintained good bioactivity. Additionally, the safety of mucosal immunity delivery system of the pFNDV-CS-NPs was also tested in vitro by cell cytotoxicity and in vivo by safety test in chickens. In vivo immunization showed that better immune responses of specific pathogen-free chickens immunized with the pFNDV-CS-NPs were induced, and prolonged release of the plasmid DNA was achieved compared to the chickens immunized with the control plasmid. This study lays the foundation for the further development of mucosal vaccines and drugs encapsulated in chitosan nanoparticles.

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Section: Introductionmentioning

confidence: 87%

Preparation and efficacy of Newcastle disease virus DNA vaccine encapsulated in chitosan nanoparticles

Zhao¹,

Zhang²,

Zhang³

et al. 2014

IJN

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“…Partículas são formadas nebulizando uma solução de quitosana em uma solução alcalina contendo hidróxido de sódio, NaOH-metanol ou dietanolamina (NISHIMURA et al, 1986). A separação e purificação das partículas obtidas podem ser feitas por filtração ou centrifugação seguida por lavagens sucessivas com água quente (KAWASHIMA et al, 1985). O TTP, ao contrário do glutaraldeído, que pode causar irritações às membranas mucosas devido a sua toxicidade, não é tóxico e forma gel por interação iônica entre os grupos aminos carregados positivamente da quitosana e os contra-íons do TTP (ARAL; AKBUGA, 1998;MI et al, 1999;ZHU, 2000ZHU, , 2001.…”

Section: Coacervação/precipitaçãounclassified

Desenvolvimento, caracterização e avaliação de sistemas microestruturados para veiculação de ácido retinóico na pele

Lira¹

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“…The release of ceftriaxone sodium chitosan nano formulation was determined using a modified dialysis membrane diffusion technique [13][14][15][16][17]. An accurately weighed amount of ceftriaxone sodium chitosan nano formulation equivalent to 50 mg of ceftriaxone sodium was transferred to a plastic cylinder having the length of 6 cm and diameter of 2.5 cm fitted at its lower end with dialysis membrane (Himedia dialysis membrane with the molecular cut off of 10 kDa) presoaked with distilled water for 24 h. The cylinder was then suspended in the dissolution flask of a USP dissolution apparatus using 900 ml of phosphate buffer solution (pH 7.2) [19,20]. The cylinder was allowed to rotate at a constant speed (25 rpm) at 37 °C±0.5.…”

Section: In Vitro Drug Release Studymentioning

confidence: 99%

Preparation and Characterization of Ceftriaxone Sodium Encapsulated Chitosan Nanoparticles

Manimekalai

Dhanalakshmi²,

Manavalan³

2017

Int J App Pharm

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Objective: The objective of this study was to prepare ceftriaxone sodium chitosan nanoparticles (CS-NP) from different drug and polymer ratios and analyze their physicochemical characteristics.Methods: Ceftriaxone sodium loaded chitosan nanoparticles were prepared using chitosan as a polymer and tri sodium polyphosphate (TPP) as cross linking agent by ionic cross linking and coacervation with the aid of sonication. Various trials have been carried out for the confirmation of nanoformulation. Parameters such as the zeta potential, polydispersity, particle size, entrapment efficiency, in vitro drug release Thermo gravimetric analysis and scanning electron microscope of the nanoparticles were assessed for confirmation of nanoformulation. Results:The formulated nanoparticles showed mean particle size, polydispersity index and zeta potential to be 183.1±8.42 nm, 0.212±0.05, +38.5±1.6 mV respectively and the drug loading was found to be 46.42±10 %. In vitro drug release was showed a biphasic release pattern with initial burst release followed by sustained release of formulated nanoparticles. The cumulative percentage of drug release was about 83.08 %. Conclusion:Formulation F2 was found to be the best formulation with a higher cumulative percentage of drug release. Modified ionic gelation method can be utilized for the development of chitosan nanoparticles of ceftriaxone sodium. Polymer and crosslinking agent concentrations and sonication time are rate-limiting factors for the development of the optimized formulation. The chitosan nanoparticles developed would be capable of sustained delivery of ceftriaxone sodium.

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Novel Method for the Preparation of Controlled-Release Theophylline Granules Coated with a Polyelectrolyte Complex of Sodium Polyphosphate-Chitosan

Cited by 153 publications

References 9 publications

Preparation and efficacy of Newcastle disease virus DNA vaccine encapsulated in chitosan nanoparticles

Preparation and efficacy of Newcastle disease virus DNA vaccine encapsulated in chitosan nanoparticles

Desenvolvimento, caracterização e avaliação de sistemas microestruturados para veiculação de ácido retinóico na pele

Preparation and Characterization of Ceftriaxone Sodium Encapsulated Chitosan Nanoparticles

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