Novel method for the investigation of the electrochemistry of metalloproteins: cytochrome c

Eddowes, M.J.; Hill, H. Allen O.

doi:10.1039/c3977000771b

Cited by 416 publications

(198 citation statements)

References 0 publications

Supporting

Mentioning

189

Contrasting

Unclassified

Order By: Relevance

“…A well resolved DPV peak is detected at 70 mV, even in 0.5 M cyt c solutions. The peak potential value as well as the dependence of the peak current on cyt c concentration indicate that the observed signal is due to the one-electron electrochemical reduction of Fe(III) of the protein heme-group [18], according to reaction (7):…”

Section: Differential Pulse Voltammetry With Nees For Trace Analysesmentioning

confidence: 98%

“…In order to check the possibility to use DPV at NEEs for trace determinations even of those analytes which are "difficult" to determine, a series of tests were performed in solutions containing the redox protein cytochrome c. Really, the electrochemistry of cyt c has been studied widely; however the usual procedure to obtain the protein electrochemistry signal is to add to the cyt c solution a suitable promoter (typically 4,4 -bipy [18,19] or 4,4 -dipirydil sulfide [20,21] or others [22,23]) or to use electrodes modified with polyanionic coatings or membranes [13,24]; in both cases the modifier interacts with the protein and orient it in a way which favors the electron transfer. A couple of examples showing the possibility to avoid the use of the promoter has been published.…”

Section: Differential Pulse Voltammetry With Nees For Trace Analysesmentioning

confidence: 99%

See 1 more Smart Citation

Voltammetry of redox analytes at trace concentrations with nanoelectrode ensembles

Moretto

Pepe

Ugo

2004

Talanta

View full text Add to dashboard Cite

Section: Differential Pulse Voltammetry With Nees For Trace Analysesmentioning

confidence: 98%

Section: Differential Pulse Voltammetry With Nees For Trace Analysesmentioning

confidence: 99%

Voltammetry of redox analytes at trace concentrations with nanoelectrode ensembles

Moretto

Pepe

Ugo

2004

Talanta

View full text Add to dashboard Cite

“…The breakthrough came in the 1970s when three groups established conditions that allowed the native state of a redox protein to approach an electrode with sufficient proximity and the appropriate orientation to support facile and direct interfacial electron exchange. [3][4][5] Numerous proteins have now been studied by dynamic electrochemistry producing results that have quantified not only behaviour recognisable to the biochemical community but also new facets of protein activity.…”

Section: Introductionmentioning

confidence: 99%

The relationship between redox enzyme activity and electrochemical potential—cellular and mechanistic implications from protein film electrochemistry

Gates

Kemp

et al. 2011

Phys. Chem. Chem. Phys.

View full text Add to dashboard Cite

In protein film electrochemistry a redox protein of interest is studied as an electroactive film adsorbed on an electrode surface. For redox enzymes this configuration allows quantification of the relationship between catalytic activity and electrochemical potential. Considered as a function of enzyme environment, i.e., pH, substrate concentration etc., the activity-potential relationship provides a fingerprint of activity unique to a given enzyme. Here we consider the nature of the activity-potential relationship in terms of both its cellular impact and its origin in the structure and catalytic mechanism of the enzyme. We propose that the activity-potential relationship of a redox enzyme is tuned to facilitate cellular function and highlight opportunities to test this hypothesis through computational, structural, biochemical and cellular studies.

show abstract

“…The cytochrome c case is noteworthy because at NEEs, well-resolved CVs are obtained in diluted solutions of the protein both with and without promoters such as 4,4Ј-bipyridyl typically used for promoting cytochrome c electrochemistry (99)(100)(101). These promoters are generally required to avoid adsorption/denaturation (83,102,103) of cytochrome c on the Au surface.…”

Section: Electroanalysis With Neesmentioning

confidence: 99%

Template Deposition of Metals

Ugo

Moretto

2007

Handbook of Electrochemistry

View full text Add to dashboard Cite

Template synthesis is a relatively simple and easy procedure which has made the fabrication of rather sophisticated nanomaterials accessible to almost any laboratory. Template synthesis requires access to instrumentation capable of metal sputtering and electrochemical deposition. The characterization of the fabricated nanostructures can be done using instrumental techniques including spectrophotometry, voltammetry, optical microscopy, atomic force microscopy, and electronic microscopies (scanning electron microscopy (SEM) and transmission electron microscopy (TEM)).The method is based on the simple but effective idea that the pores of a host material can be used as a template to direct the growth of new materials. Historically, template synthesis was introduced by Possin (1) and refined by Williams and Giordano (2) who prepared different metallic nanowires with widths as small as 10 nm within the pores of etched nuclear damaged tracks in mica. It was further developed by Martin's group (3-5) and followed by others (6) with the number of examples and applications (7) continually increasing. The nanoporous membranes usually employed as templates are alumina or track-etched polymeric membranes which are widely used as ultrafiltration membranes. Recently, metal nanostructures have also been obtained using the pores created by self-assembly in block copolymer structures under the influence of electric fields and high temperatures (8, 9).The first part of this section focuses on the main characteristics and fabrication techniques used for obtaining templating membranes and depositing metal nanostructures by suitable electroless and electrochemical procedures. Methods such as sol-gel (10-12) or chemical vapor deposition (10, 13), which have been used primarily for the template deposition of carbon, oxides, or semiconducting-based materials, will not be considered here in detail. The second part of the section focuses on the electrochemical properties of the fabricated nanomaterials with emphasis on the characteristics and applications of nanoelectrode ensembles (NEEs). Templating membranes 16.2.2

show abstract

Novel method for the investigation of the electrochemistry of metalloproteins: cytochrome c

Cited by 416 publications

References 0 publications

Voltammetry of redox analytes at trace concentrations with nanoelectrode ensembles

Voltammetry of redox analytes at trace concentrations with nanoelectrode ensembles

The relationship between redox enzyme activity and electrochemical potential—cellular and mechanistic implications from protein film electrochemistry

Template Deposition of Metals

Contact Info

Product

Resources

About