Novel method for the generation of tissue-engineered vascular grafts based on a highly compacted fibrin matrix

Aper, Thomas; Wilhelmi, Mathias; Gebhardt, C.; Hoeffler, Klaus; Benecke, Nils; Hilfiker, Andres; Haverich, Axel

doi:10.1016/j.actbio.2015.10.012

Cited by 70 publications

(52 citation statements)

References 31 publications

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“…In this way, a regenerative, biological implant, which will not be attacked immunologically by the host, is converted successively to truly endogenous tissue in the course of the physiological remodelling. Tubular structures of fibrin and collagen can be constructed in vitro as an alternative to decellularisation of preformed donor tissue [26,72].…”

Section: The Ideal Vascular Prosthesismentioning

confidence: 99%

Cardiovascular Applications of Magnesium Alloys

Schilling¹,

Bauer²,

LaLonde³

et al. 2017

Magnesium Alloys

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Therapy in cardiovascular medicine often relies on implantation of prosthetic materials or application of stents. The diseases of many cardiovascular structures require their complete and immediate repair by utilising prosthetic materials. The ideal cardiovascular prosthesis involves good functional properties, capability of regeneration and does not activate the host's immune system. Ideally, the graft can be applied for a temporary use and degrades after a predefined period according to controlled degradation kinetics. Only biological grafts would provide this spectrum of properties by today's level of knowledge. However, biological prostheses exhibit some relevant drawbacks as well, such as insufficient mechanical stability or restricted availability. Implants or supporting structures of magnesium alloys would bridge this gap and would either provide a substrate for innovative and temporary grafts or would-as supporting structures-transiently add some missing properties to regenerative biological prostheses. This chapter reviews the different fields of cardiovascular therapeutic applications of magnesium alloys. The required properties of magnesium alloys and their preparation, fabrication and testing will be discussed under the specific cardiovascular perspective.

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Section: The Ideal Vascular Prosthesismentioning

confidence: 99%

Cardiovascular Applications of Magnesium Alloys

Schilling¹,

Bauer²,

LaLonde³

et al. 2017

Magnesium Alloys

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“…30 The number of cells which are capable to differentiate into EC in vitro is estimated to only 0.05–0.2 cells per mL blood. 31 Thus, in many applications, an expansion of the cells will be necessary in vitro in order to obtain a sufficiently large number of cells.…”

Section: Discussionmentioning

confidence: 99%

Outgrowing endothelial and smooth muscle cells for tissue engineering approaches

et al. 2017

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In recent years, circulating progenitors of endothelial cells and smooth muscle cells were identified in the peripheral blood. In our study, we evaluated the utilization of both cell types isolated and differentiated from peripheral porcine blood in terms for their use for tissue engineering purposes. By means of density gradient centrifugation, the monocyte fraction from porcine blood was separated, split, and cultivated with specific culture media with either endothelial cell growth medium-2 or smooth muscle cell growth medium-2 for the differentiation of endothelial cells or smooth muscle cells. Obtained cells were characterized at an early stage of cultivation before the first passage and a late stage (fourth passage) on the basis of the expression of the antigens CD31, CD34, CD45, nitric oxide synthase, and the contractile filaments smooth-muscle alpha-actin (sm-alpha-actin) and smoothelin. Functional characterization was done based on the secretion of nitric oxide, the formation of a coherent monolayer on polytetrafluoroethylene, and capillary sprouting. During cultivation in both endothelial cell growth medium-2 and smooth muscle cell growth medium-2, substantially two types of cells grew out: early outgrown CD45-positive cells, which disappeared during further cultivation, and in 85% (n = 17/20) of cultures cultivated with endothelial cell growth medium-2 colony-forming late outgrowth endothelial cells. During cultivation with smooth muscle cell growth medium-2 in 80% (n = 16/20) of isolations colony-forming late outgrowth smooth muscle cells entered the stage. Cultivation with either endothelial cell growth medium-2 or smooth muscle cell growth medium-2 had selective effect on the late outgrown cells to that effect that the number of CD31-positive cells increased from 34.8% ± 13% to 83.9% ± 8% in cultures cultivated with endothelial cell growth medium-2 and the number of sm-α-actin+ cells increased from 52.6% ± 18% to 88% ± 5% in cultures cultivated with smooth muscle cell growth medium-2, respectively. Functional analyses revealed significantly higher levels of nitric oxide secretion, endothelialization capacity, and capillary formation in not expanded cultures cultivated with endothelial cell growth medium-2 in comparison to later stages of cultivation and mature aortic cells. Blood seems to be a reliable and feasible source for the isolation of both endothelial and smooth muscle cells for application in tissue engineering approaches. Whereas, early co-cultures of early and late outgrowth cells provide functional advantages, the differentiation of cells can be directed selectively by the used culture medium for the expansion of highly proliferative late outgrowth endothelial cells and late outgrowth smooth muscle cells, respectively.

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“…Currently, the ideal approach in tissue regeneration and repair should be minimal invasive, therefore, not involving autologous tissue grafts. In these terms, polymer devices promoting cell proliferation, and able to guide tissue regeneration, are particularly interesting; their principal objective is to recapitulate extracellular matrix (ECM) function in a temporarily coordinated and spatially organized structure …”

Section: Introductionmentioning

confidence: 99%

Design of a Bioabsorbable Multilayered Patch for Esophagus Tissue Engineering

Dorati

Trizio

Marconi

et al. 2017

Macromolecular Bioscience

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A gold standard for esophagus reconstruction is not still available. The present work aims to design a polymer patch combining synthetic polylactide-co-polycaprolacton and chitosan biopolymers, tailoring patch properties to esophageal tissue characteristics by a temperature-induced precipitation method, to get multilayered patches (1L, 2L, and 3L). Characterization shows stable multilayered patches (1L and 2L) by selection of copolymer type, and their M . In vitro investigation of the functional patch properties in simulated physiologic and pathologic conditions demonstrates that the chitosan layer (patch 3L) decreases patch stability and cell adhesion, while improves cell proliferation. Patches 2L and 3L comply with physiological esophageal pressure (3-5 kPa) and elongation (20%).

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Novel method for the generation of tissue-engineered vascular grafts based on a highly compacted fibrin matrix

Cited by 70 publications

References 31 publications

Cardiovascular Applications of Magnesium Alloys

Cardiovascular Applications of Magnesium Alloys

Outgrowing endothelial and smooth muscle cells for tissue engineering approaches

Design of a Bioabsorbable Multilayered Patch for Esophagus Tissue Engineering

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