Novel method for the cryopreservation of testicular sperm and ejaculated spermatozoa from patients with severe oligospermia: A pilot study

Cryopreservation of Human Spermatozoa by Vitrification vs. Slow Freezing: Canadian Experience

“…Straws can be made of polyethylene terephthalate glycol (PETG) or ionomeric resin (CBS High Security Straws by CryoBioSystem, Paris, France). As described above, a low number or single spermatozoa have been experimentally frozen in empty animal or human zona pellucida, spheres of Volvox Globator algae, alginate agarose bead microspheres and ICSI pipettes (AbdelHafez et al, 2009;Herrler et al, 2006;Isaev et al, 2007;Just et al, 2004;Walmsley et al, 1998). For vitrification purposes different types of storing strategies have been suggested.…”

“…While this method requires special skills, equipment, and is very labour-intensive; live births were reported using both human and hamster zona (Walmsley et al, 1998). Spermatozoa were also injected and frozen within spheres of Volvox Globator algae and recovered after thawing using an ICSI needle (Just et al, 2004). While all of these methods appear to be attractive for single spermatozoa cryopreservation, they have a number of limitations.…”

Effect of sperm storage and selection techniques on sperm parameters

“…Just et al [2004] showed that a small number of sperm can be cryopreserved in these colonies. Selected sperm were introduced into the spheres in cryoprotectants using an ICSI pipette and were then frozen using a conventional sperm freezing protocol.…”

“…The post-thaw recovery rate was 100% and the motility rate was approximately 60%. With this method, however, human gametes are exposed to algae cells, which is restricted by new FDA and European tissue directive regulations [AbdelHafez et al 2009;Just et al 2004].…”

Genome Banking for Vertebrates Wildlife Conservation

“…When the initial sample is of very poor quality or with very small number of cells, small cell-number or single cell cryopreservation techniques may become useful. Starting in the late 1990's , reports on several single sperm cryopreservation techniques showed up in the scientific literature (Walmsley et al, 1998;Gil-Salom et al, 2000;Gvakharia & Adamson, 2001;Just et al, 2004;Herrler et al, 2006;Isaev et al, 2007;Koscinski et al, 2007;Woods et al, 2010). Using these various techniques, researchers reported a wide range of outcomes and recovery efficiency.…”