Novel method for renal preservation at subzero temperatures

Tomalty, Heather E.; Hamilton, Alan; Hamilton, Erin F.; Kukal, Olga; Allen, Tyler A.; Walker, Virginia K.

doi:10.1016/j.cryobiol.2016.09.152

Cited by 1 publication

(2 citation statements)

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“…However, it was demonstrated that preservation of neonatal rat kidneys at −2°C for 48 h in histidine-tryptophan-ketoglutarate (HTK) solution at −2°C reduced histological damage (cortex and medulla) compared to 24 or 4°C ( 43 ). Consistently, rat kidneys stored in cryostasis storage solution with or without the addition of a hyperactive insect antifreeze protein for 48 h and 72 h, respectively, demonstrated similar viability (MTT reduction assays, caspase-3 and calpain activity) compared to kidneys stored for 24 h in UW ( 41 ).…”

Section: Supercooling Principles To Extend Organ Preservationmentioning

confidence: 68%

“…Dimethyl sulfoxide (DMSO) has also been widely used for various applications in cryobiology but seems to provoke cell toxicity ( 85 ). As for extracellular CPAs, needed to prevent the intravascular compartment and the surrounding media from freezing, most of the preservation protocols in solid organs have been using polyols and polyethers such as glycerol and polyethylene glycol (PEG) ( 86 – 41 ). Nature-inspired molecules like Trehalose, a complex sugar used by plants to avoid freezing, have also been experimented with ( 88 , 89 ).…”

Section: Supercooling Principles To Extend Organ Preservationmentioning

confidence: 99%

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Supercooling: a promising technique for prolonged preservation in solid organ transplantation, and early perspectives in vascularized composite allografts

Berkane,

Hayau,

Filz von Reiterdank

et al. 2023

Front. Transplant.

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Ex vivo preservation of transplanted organs is undergoing spectacular advances. Machine perfusion is now used in common practice for abdominal and thoracic organ transportation and preservation, and early results are in favor of substantially improved outcomes. It is based on decreasing ischemia-reperfusion phenomena by providing physiological or sub-physiological conditions until transplantation. Alternatively, supercooling techniques involving static preservation at negative temperatures while avoiding ice formation have shown encouraging results in solid organs. Here, the rationale is to decrease the organ's metabolism and need for oxygen and nutrients, allowing for extended preservation durations. The aim of this work is to review all advances of supercooling in transplantation, browsing the literature for each organ. A specific objective was also to study the initial evidence, the prospects, and potential applications of supercooling preservation in Vascularized Composite Allotransplantation (VCA). This complex entity needs a substantial effort to improve long-term outcomes, marked by chronic rejection. Improving preservation techniques is critical to ensure the favorable evolution of VCAs, and supercooling techniques could greatly participate in these advances.

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Section: Supercooling Principles To Extend Organ Preservationmentioning

confidence: 68%